Induced spawning in perch, Perca fluviatilis L. using carp pituitary extract and HCG

Abstract

Induced spawning in Eurasian perch, Perca fluviatilis L., was studied using human chorionic gonadotrophs (hCG) or acetone‐dried common carp pituitary with or without hCG. The dose administered to fish was 5700 IU hCG kg‐1 or 4.0 mg kg‐1 carp pituitary with or without 500‐700 IU hCG kg‐1 for females and 2850 IU hCG kg‐1 or 2.0 mg kg‐1 carp pituitary with or without 250‐350 IU hCG kg‐1 for males. There were no statistically significant differences in quantity of milt in treated and control groups, although the best result was observed when males were treated with a triple injection of hCG and carp pituitary extract. Male spermiating success, expressed as a quantity of milt, was negatively correlated with fish weight. All females treated in this experiment had oocytes at the same division, so time of ovulation was highly synchronous. Spawning success, expressed as a spawning effectivity coefficient (Sc), was highest in fish treated with the triple injection. Spawning methods described in this paper were successful, even though hormones from another order of fish were used.

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“…The low effects of hormonal stimulation of these heterologous hormones have already been reported in some cyprinids which spawn only once during the season, such as common bream Abramis brama (L.) (Kucharczyk et al, 1997a;1997b, Kucharczyk et al, 2005, ide (Krejszeff et al, 2009;Targońska-Dietrich et al, 2004;Jamróz et al, 2008) chub Krejszeff et al, 2010) or common carp (Yaron et al, 2009). However, hCG has been reported to be effective in other species, mostly cyprinids with batch spawning (Kucharczyk et al, 1997c;Krejszeff et al, 2010) and Percids (Kucharczyk et al, 1996;Ronyai, 2007). The positive effect of subsequent stimulation with Ovopel in females previously treated with hCG indicates that the failure is not due to an inadequate state of the fish ovary, but rather a lack of response of this species to hCG and OVA-RH.…”

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“…In the case of artificial reproduction of perch, different hormonal preparations have been tested in order to synchronize ovulation during the reproductive season (Kucharczyk et al, 1996;1998;Kouril et al, 1997) and to induce ovulation out-of the reproductive season (Migaud et al, 2004;Szczerbowski et al, 2009). The data presented in this paper indicates that the farmers should also consider another factor, i.e.…”

“…In the case of perch artificial reproduction, wild fish are usually obtained during the reproductive season (Kucharczyk et al, 1996;1998;Ronyai and Lengyel, 2010;Żarski et al, 2011). However, the stage of maturity of females is then very diversified (Kucharczyk et al, 1998) which hinders the possibility of ovulation synchronization.…”

Induced spawning in perch, Perca fluviatilis L., using FSH + LH with pimozide or metoclopramide

“…individual marking and oocyte maturation checking) were made using the methods described by Kucharczyk et al (1996a). Fish were selected according to the following criteria: the belly of females had to be fully distended, and bulging, soft and resilient to touch; and males had to be spermiating.…”

“…Perch is a species which can be spawned easily under artificial conditions (Flajshans & Gondor 1989;Kucharczyk, Kujawa, Mamcarz, Skrzypczak & Wyszomirska 1996a). Perch is a species which can be spawned easily under artificial conditions (Flajshans & Gondor 1989;Kucharczyk, Kujawa, Mamcarz, Skrzypczak & Wyszomirska 1996a).…”