Induced Sputum Analysis in Subjects With Systemic Sclerosis

Litinsky, Irena; Fireman, Elizabeth; Paran, Daphna; Polachek, Ari; Broide, Adi; Sharabi, Amir; Anouk, Marina; Elkayam, Ori

doi:10.4187/respcare.04706

Cited by 4 publications

(8 citation statements)

References 22 publications

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“…This heterogeneity of results might be related to the presence of concomitant immunosuppresive therapies, 44% of our patients, 45% in Litinsky’s work and only in 25% of Damjanov’s one, but also to the type of controls chosen. Our observation is therefore close to the findings of Litinsky et al 24 .…”

Section: Discussionsupporting

confidence: 93%

“…Therefore, induced sputum (IS), which is less invasive and of clinical interest for airway diseases such as idiopathic pulmonary fibrosis (IPF) 20 – 23 , can be proposed as a good non-invasive tool to study lung inflammation in patients with SSc. Previous researches using IS and BALF in SSc have mainly focused on the cellularity 24 – 26 . If many biomarkers have already been studied both in serum and BALF from ILD patients and in particular in IPF 22 , 27 , very few works have been done in SSc as recently reviewed 28 .…”

Section: Introductionmentioning

confidence: 99%

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Inflammatory profile of induced sputum composition in systemic sclerosis and comparison with healthy volunteers

Jacquerie

Henket

Andre

et al. 2021

Sci Rep

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Systemic sclerosis (SSc) is a potentially serious and disabling connective tissue disease specially in case of interstitial lung disease (SSc-ILD). The aim of our study was to evaluate the potential utility of dosing in the induced sputum (IS) and to compare their levels in SSc-ILD and SSc-nonILD patients, as well as in healthy volunteers (HV). IS and sera values were also compared. In a prospective cross-sectional analysis, we studied the IS and serum provided from 25 SSc patients, 15 SSc-nonILD and 10 SSc-ILD, compared to 25 HV. We analyzed sputum cell composition and quantified in the supernatant and corresponding serum by commercially available immunoassays: IGFBP-1, IGFBP-2, IGFBP-3, TGF-β, IL-8, TNF-α, YKL-40, MMP-7 and MMP-9. Lung function was studied by the determination of FEV-1 (%), FVC (%), DLCO (%) and KCO (%). The IS of SSc patients had a lower weight than HV (p<0.05, p<0.01) without any significant difference with regard to the cellularity. IGFBP-1 (p < 0.0001), TGF-β (p < 0.05), IL-8 (p < 0.05), YKL-40 (p < 0.0001) and MMP-7 (p < 0.01) levels were increased in the IS of SSc patients compared to HV. Only IL-8 serum levels (p < 0.001) were increased in SSc patients compared to HV. Neither in IS nor in serum were observed differences between SSc-ILD and SSc-nonILD patients. Correlations were observed between IS IL-8 levels and FEV-1 (%) (r = = − 0.53, p < 0.01), FVC (%) (r = − 0.51, p < 0.01) and annualized ∆KCO (%) (r = 0.57, p < 0.05), between IS TGF-β levels and annualized ∆FEV-1 (%) (r = = − 0.57, p < 0.05), between IS IGFBP-2 levels and annualized ∆KCO (%) (r = 0.56, p < 0.05). Our study showed that SSc patients exhibit raised IS levels of IGFBP-1, TGF-β, IL-8, YKL-40 and MMP-7, molecules known to be involved in lung remodeling and fibrotic process, without any significant difference between SSc-ILD and SSc-nonILD patients. IL-8, TGF-β and IGFBP-2 are correlated with lung function in SSc patients which emphasize clinical relevance. IS analysis represents a new approach to understand lung inflammatory process in SSc patients. A longitudinal study is needed to evaluate their pathophysiological relevance.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Inflammatory profile of induced sputum composition in systemic sclerosis and comparison with healthy volunteers

Jacquerie

Henket

Andre

et al. 2021

Sci Rep

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show abstract

Section: Discussionsupporting

confidence: 93%

“…Yialmaz et al [27] studying the IS of 17 SSc-ILD patients (versus 12 non-ILD pulmonary controls) observed a significant increase in total number of inflammatory cells, of lymphocytes and macrophages, and lower neutrophils, in SSc patients. Litinsky et al [25] studying the IS of 20 SSc-patients (versus 16 HV) reported an isolated decrease in lymphocytes (p=0.045), with no change in the percentage of neutrophils, macrophages and eosinophils. This heterogeneity of results might be related to the presence of concomitant immunosuppresive therapies, 44% of our patients, 45% in Litinsky’s work and only in 25% of Damjanov’s one, but also to the type of controls chosen.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, induced sputum (IS), which is less invasive and of clinical interest for airway diseases such as idiopathic pulmonary fibrosis (IPF)[21-24], can be proposed as a good non-invasive tool to study lung inflammation in patients with SSc. Previous researches using IS and BALF in SSc have mainly focused on the cellularity [25-27]. If many biomarkers have already been studied both in serum and BALF from ILD patients and in particular in IPF [23,28], very few works have been done in SSc as recently reviewed [29].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inflammatory profile of induced sputum composition in systemic sclerosis: a comparison with healthy volunteers

Jacquerie

Henket

Andre

et al. 2020

Preprint

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BackgroundSystemic sclerosis (SSc) is a potentially serious and disabling connective tissue disease specially in case of interstitial lung disease (SSc-ILD). The aim of our study was to evaluate the potential utility of dosing in the induced sputum (IS) and to compare their levels in SSc-ILD and SSc-nonILD patients, as well as in healthy volunteers (HV). IS and sera values were also compared.MethodsIn a prospective cross-sectional analysis, we studied the IS and serum provided from 25 SSc patients, 15 SSc-nonILD and 10 SSc-ILD, compared to 25 HV. We analyzed sputum cell composition and quantified in the supernatant and corresponding serum by commercially available immunoassays: IGFBP-1, IGFBP-2, IGFBP-3, TGF-β, IL-8, TNF-α, YKL-40, MMP-7 and MMP-9. Lung function was studied by the determination of FEV-1 (%), FVC (%), DLCO (%) and KCO (%).ResultsThe IS of SSc patients had a lower weight than HV (p<0.01) without any significant difference with regard to the cellularity. IGFBP-1 (p<0.0001), TGF-β (p<0.05), IL-8 (p<0.05), YKL-40 (p<0.0001) and MMP-7 (p<0.01) levels were increased in the IS of SSc patients compared to HV. Only IL-8 serum levels (p<0.001) were increased in SSc patients compared to HV. Neither in IS nor in serum were observed differences between SSc-ILD and SSc-nonILD patients. Correlations were observed between IS IL-8 levels and FEV-1 (%)(r=-0.53, p<0.01), FVC (%) (r=-0.51, p<0.01) and annualized □KCO (%) (r=0.57, p<0.05), between IS TGF-□ levels and annualized □FEV-1 (%) (r=-0.57, p<0.05), between IS IGFBP-2 levels and annualized □KCO (%) (r=0.56, p<0.05).ConclusionOur study showed that SSc patients exhibit raised IS levels of IGFBP-1, TGF-β, IL-8, YKL-40 and MMP-7, molecules known to be involved in lung remodeling and fibrotic process, without any significant difference between SSc-ILD and SSc-nonILD patients. IL-8, TGF-□ and IGFBP-2 are correlated with lung function in SSc patients which emphasize clinical relevance. IS analysis represents a new approach to understand lung inflammatory process in SSc patients. A longitudinal study is needed to evaluate their pathophysiological relevance.

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Sputum quantitative cytometry in patients with interstitial lung disease and chronic cough

Scallan

Rezaee

et al. 2020

Respiratory Medicine

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Induced Sputum Analysis in Subjects With Systemic Sclerosis

Abstract: Induced sputum analysis of subjects with systemic sclerosis, including those with restrictive lung disease, shows changes in cellular pattern and correlation with several highly relevant clinical and PFT parameters.

Cited by 4 publications

References 22 publications

Inflammatory profile of induced sputum composition in systemic sclerosis and comparison with healthy volunteers

Inflammatory profile of induced sputum composition in systemic sclerosis and comparison with healthy volunteers

Inflammatory profile of induced sputum composition in systemic sclerosis: a comparison with healthy volunteers

Sputum quantitative cytometry in patients with interstitial lung disease and chronic cough

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