Induced sputum and bronchoalveolar lavage as tools for evaluating the effects of inhaled corticosteroids in patients with asthma

Nocker, R. E. T.; Out, Theo A.; Weller, F. R.; Riemer, M.J. de; Jansen, Henk M.; Zee, Jaring S. van der

doi:10.1067/mlc.2000.107305

Cited by 23 publications

(19 citation statements)

References 27 publications

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“…Leakage of plasma proteins is a hallmark of allergy and inflammation due to increased permeability of the vasculature. In patients with asthmatic, significant increase in a2‐Macroglobulin and albumin in BAL fluid and serum were observed after segmental allergen challenge . a2‐Macroglobulin increase was also reported in persistent allergic rhinitis .…”

Section: Discussionmentioning

confidence: 77%

Identification of unique proteomic signatures in allergic and non‐allergic skin disease

Wang¹,

Suárez‐Fariñas

Estrada

et al. 2017

Clin Experimental Allergy

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Summary Background Atopic dermatitis (AD), psoriasis (PS), and contact dermatitis (CD) are common skin diseases, characterized by barrier disruption and systemic inflammation, with unique epidermal signatures and common inflammatory pathways identified by transcriptomic profiling. This study profiled proteomic signatures in serum from subjects with AD, PS, and CD compared with healthy controls (HC). Objective Identify unique proteomic signatures to distinguish between inflammatory diseases with similar epidermal disruption and overlapping epithelial inflammation. Methods Sera from 20 subjects with moderate to severe AD, 10 subjects with CD, 12 subjects with moderate to severe PS, 10 subjects with both AD and CD, and 10 HC with no history of skin disease was analysed using high‐throughput proteomic analysis that detects expression of 1129 protein targets. Protein expression was compared between disease and HC, and across diseases for statistical significance (fold change≥1.5 and false discovery rate≤0.05), to identify unique proteomic signatures for each disease. Results Complement C5A anaphylatoxin (C5A), lipopolysaccharide binding protein (LBP), C‐reactive protein (CRP), ILT‐4, C‐C motif ligand 18 (PARC), and sialic acid‐binding Ig‐like lectin 14 (SIG14) were significantly modulated in all three diseases compared with HC. We identified unique signatures for AD (Immunoglobulin E (IgE), thymus‐ and activation‐regulated chemokine (TARC) and macrophage‐derived chemokine (MDC)), CD (10 proteins), and PS (kynureninase (KYNU)). Proteomic profiling in subjects with both AD and CD identified additional dysregulated proteins compared with subjects with either condition alone, indicating an exacerbated inflammation reaction. Conclusions and Clinical Relevance Unique sera proteomic signatures may distinguish between inflammatory skin diseases despite similar epidermal barrier disruption and epithelial inflammation. This may provide insight into disease pathogenesis, diagnosis, and therapeutic intervention in difficult‐to‐treat subjects.

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Section: Discussionmentioning

confidence: 77%

Identification of unique proteomic signatures in allergic and non‐allergic skin disease

Wang¹,

Suárez‐Fariñas

Estrada

et al. 2017

Clin Experimental Allergy

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“…Sputum induction and processing of whole sputum samples were performed according the method described by Fahy et al 16 with minor modifications as described previously. 17 Differential cell counts were expressed as percentage of cells, excluding squamous epithelial cells. Sputum samples containing more than 80% squamous cells on differential cell counting were excluded from analysis.…”

Section: Methodsmentioning

confidence: 99%

Adding salmeterol to an inhaled corticosteroid reduces allergen-induced serum IL-5 and peripheral blood eosinophils

Koopmans¹,

Lutter

Jansen³

et al. 2005

Journal of Allergy and Clinical Immunology

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“…Sputum induction and processing were performed as described earlier [28]. Shortly, induction was performed by inhalation of nebulized hypertonic saline (NaCl) during quiet tidal breathing.…”

Section: Methodsmentioning

confidence: 99%

Serum surfactant protein D is elevated in allergic patients

Koopmans¹,

Zee²,

Krop³

et al. 2004

Clin Experimental Allergy

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Induced sputum and bronchoalveolar lavage as tools for evaluating the effects of inhaled corticosteroids in patients with asthma

Cited by 23 publications

References 27 publications

Identification of unique proteomic signatures in allergic and non‐allergic skin disease

Identification of unique proteomic signatures in allergic and non‐allergic skin disease

Adding salmeterol to an inhaled corticosteroid reduces allergen-induced serum IL-5 and peripheral blood eosinophils

Serum surfactant protein D is elevated in allergic patients

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