Induced Tetraploidy in a Vaccinium elliottii Facilitates Crossing with Cultivated Highbush Blueberry

Dweikat, I. M.; Lyrene, P. M.

doi:10.21273/jashs.116.6.1063

Cited by 16 publications

(15 citation statements)

References 10 publications

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“…Abnormal cytological behavior of the PMCs of an autotetraploid Vaccinium elliottii Chapm. also resulted with reduced pollen viability (Dweikat and Lyrene, 1991;Vorsa and Ballington, 1991). This suggests the widespread occurrence of abnormal meiotic behavior and pollen viability among plants.…”

Section: Discussionmentioning

confidence: 90%

Meiotic Chromosome Behavior and Capsule Setting in Doritaenopsis Hybrids

Bolaños-Villegas¹,

Chin²,

Chen³

2008

J. Amer. Soc. Hort. Sci.

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ADDITIONAL INDEX WORDS. pollen viability, orchid breeding ABSTRACT. The development of new cultivars in Doritaenopsis Guillaum. & Lami orchids is often hindered by factors such as low seed count in hybrids. Cytological study may offer the ability to develop new hybrids by revealing cultivars with good chromosome pairing and high pollen viability, which are somewhat difficult to obtain under current breeding programs. Cross pollination, pollen viability, and chromosomal behavior during meiosis were analyzed to reveal the relation between seed fertility and capsule set in Doritaenopsis hybrids. The number of mature capsules harvested and their relative seed content were used as indices of crossing availability. The results of meiosis were evaluated according to pollen viability detected by fluorescein diacetate and quantification of sporad types by acid fuchsin staining. Chromosome number and pairing at meiosis were observed in root tips or in samples of pollen mother cells. A positive relation was found among high seed set, high frequency of viable tetrads, high degree of chromosome pairing, and low frequency of chromosomal aberrations such as inversions and translocations. On the basis of these factors, three types of hybrids could be distinguished. In type one hybrids, chromosomes paired as bivalents, pollen mother cells divided into tetrads, and capsule setting occurred after pollination of pollen acceptors. In type two hybrids, chromosomes remained mainly as univalents that developed into micromeiocytes, pollen mother cell division was disrupted, and seed recovery was low after pollination. Type three hybrids showed chromosomes paired mostly as multivalents, chromosome bridges, pollen mother cell division with massive failure, and little fertility. In Doritaenopsis orchids, high pollen viability and high fertility depends on a high frequency of normal tetrads, and low seed set in cross-pollination is predicted with micronuclei in the end products of meiosis. The occurrence of chromosomal aberrations may suggest a process of genome differentiation that could compromise breeding efforts if not taken into consideration.

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Section: Discussionmentioning

confidence: 90%

Meiotic Chromosome Behavior and Capsule Setting in Doritaenopsis Hybrids

Bolaños-Villegas¹,

Chin²,

Chen³

2008

J. Amer. Soc. Hort. Sci.

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“…In diploidization as classically described, the number of bivalents increases in succeeding generations because pairing becomes restricted to specific sets of two homologous chromosomes as a result of mutations and structural rearrangements. There is circumstantial evidence in blueberry that chromosomal diploidization can occur, as US 75 had significantly more multivalents than native V. corymbosum, and a colchicine-induced tetraploid of V. elliottii was previously shown to have significantly more multivalents than tet- raploid V. corymbosum (Dweikat and Lyrene, 1991). It is interesting to note that US 75s had fewer multivalents than US 75 after only one round of selfing (2.68 vs. 2.19), although only two individuals were sampled and the difference was not significant.…”

Section: Discussionmentioning

confidence: 99%

“…Tetraploid V. corymbosum has been shown to have tetrasomic inheritance (Krebs and Hancock, 1989), even though bivalent pairing dominates during meiosis (Jelenkovic and Hough, 1970;Jelenkovic and Harrington, 1971). In fact, predominantly bivalent associations at diakinesis and metaphase I have been observed in all tetraploid materials examined to date, except in an artificially doubled genotype of V. elliottii (Dweikat and Lyrene, 1991). These bivalent associations were interpreted to be the result of obligatory pairing and localized distal chiasma (Jelenkovic and Hough, 1970).…”

mentioning

confidence: 99%

Evolution in an autopolyploid group displaying predominantly bivalent pairing at meiosis: genomic similarity of diploid Vaccinium darrowi and Autotetraploid V. corymbosum (Ericaceae)

Qu¹,

Hancock

Whallon

1998

American J of Botany

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The genomic relationship between V. darrowi Camp (2n = 2x = 24) and V. corymbosum L. (2n = 4x = 48) was examined using an interspecific tetraploid hybrid, US 75, and representatives of the parental species. Two features in the background of US 75 led to the prediction that it was an allopolyploid: (1) the parental species are quite distinct morphologically and geographically, and (2) the diploid genome was incorporated into US 75 via an unreduced gamete. However, US 75 recently was shown to display tetrasomic inheritance using molecular markers. In the present cytological study, US 75 was found to have a lower than expected number of multivalents for an autopolyploid, although it had a significantly higher number of quadrivalents than its autotetraploid parent, V. corymbosum. Normal chromosome distributions were observed at anaphase I and II, and pollen viability was high. Our findings suggest that little genomic divergence has developed between the Vaccinium species and that the polyploids may freely exchange genes with sympatric diploid species via unreduced gametes. This pattern of hybridization could be an important component of evolution in all autopolyploid groups, making them much more dynamic than traditionally assumed.

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“…A strong triploid block makes it hard to cross diploid and tetraploid species within the section, but tetraploid plants of the diploid species, produced with colchicine (Dweikat and Lyrene, 1991;Perry and Lyrene, 1984), can readily be crossed with the tetraploid species, and the resulting hybrids are vigorous and fertile.…”

mentioning

confidence: 99%

Ploidy, Fertility, and Phenotypes of F1 Hybrids between Tetraploid Highbush Blueberry Cultivars and Diploid Vaccinium elliottii

Norden¹,

Lyrene²,

Chaparro³

2020

horts

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A progeny of 55 blueberry seedlings produced by pollinating 4301 flowers of tetraploid highbush blueberry cultivars with pollen from 19 different diploid Vaccinium elliottii plants was studied to determine hybridity and ploidy. Of the 21 seedlings whose phenotypes were intermediate between parental types, indicating hybridity, 18 were triploid and three were tetraploid. Pollen of the triploids, when viewed at ×250, was almost all shrunken and aborted, although some triploid hybrids produced a few large, plump microspores in dyads or monads. Triploids produced no seed when pollinated with pollen from 4x highbush or 2x V. elliottii or when open-pollinated outside the greenhouse in the presence of fertile diploid and tetraploid blueberries. Tetraploid hybrids produced large populations of vigorous seedlings when intercrossed. Both triploid and tetraploid F1 hybrids were intermediate between the parents in leaf size and flower size. The triploids produced no berries; the tetraploids were intermediate between the parents in berry size but averaged lower in Brix and berry firmness than either parent. Seven additional F1 hybrids from reciprocal crosses were obtained by pollinating 2309 flowers of 2x V. elliottii with pollen from tetraploid highbush cultivars. Although five V. elliottii clones served as female parents in these crosses, only one produced any seedlings. Six of the seven hybrids flowered and were fertile tetraploids; one was a sterile triploid.

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Induced Tetraploidy in a Vaccinium elliottii Facilitates Crossing with Cultivated Highbush Blueberry

Cited by 16 publications

References 10 publications

Meiotic Chromosome Behavior and Capsule Setting in Doritaenopsis Hybrids

Meiotic Chromosome Behavior and Capsule Setting in Doritaenopsis Hybrids

Evolution in an autopolyploid group displaying predominantly bivalent pairing at meiosis: genomic similarity of diploid Vaccinium darrowi and Autotetraploid V. corymbosum (Ericaceae)

Ploidy, Fertility, and Phenotypes of F1 Hybrids between Tetraploid Highbush Blueberry Cultivars and Diploid Vaccinium elliottii

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