Inducible Cadmium Binding Complexes of Cabbage and Tobacco

Wagner, George J.; Trotter, Melvin M.

doi:10.1104/pp.69.4.804

Cited by 88 publications

(30 citation statements)

References 14 publications

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“…The above data and a very high degree of specificity in the response of plants to particulate metals (8) suggest that proteins play a role in tolerance mechanisms. These mechanisms can function in two ways: either the metal is metabolized into stable metaloproteins, like cadmium in cabbage (Brassica oleracea L.) (24) or it is complexed. In the latter case, proteins can serve as specific carriers.…”

Section: Methodsmentioning

confidence: 99%

“…The modified 'pulse' method was used for screening varieties and hybrid populations of wheat (2). The Al concentration in nutrient solution that caused irreversible damage of root apical meristem in 4-d-old seedlings during 24 were added at concentrations of 2.5 uCi/ml (4 ,ug/ml) and 10 ,uCi/ml (10 The distribution of Al in root subcellular fractions from induced and control Atlas 66 seedlings was studied using the methods described earlier (20). Significantly more Al was found in all fractions from pretreated roots than in controls (Table IV), but the relative distribution of Al between subcellular fractions was unchanged.…”

Section: Methodsmentioning

confidence: 99%

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Induction of Aluminum Tolerance in Wheat Seedlings by Low Doses of Aluminum in the Nutrient Solution

Aniol¹

1984

Plant Physiol.

122

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Aluminum ions are regarded as the main toxic factor in mineral acid soils of pH below 5.0 (9). Plants differ in their reaction to Al toxicity, and variability was found between plant species as well as between cultivars of cultivated plants (1,7,10). The plant reaction to Al was found to be genetically controlled (5, 23).Studies have concentrated mainly on the mechanisms of Al toxicity, especially on the inhibitory effect of Al on root elongation. According to Matsumoto et al. (17) binding to nuclear DNA is a primary effect of Al in the plant cell. However, it is not certain whether disturbances of the uptake, transport, and utilization of mineral nutrients (6, 9) or the effect on oxidation processes (13) were added at concentrations of 2.5 uCi/ml (4 ,ug/ml) and 10 ,uCi/ml (10 Ag/ml), respectively, to the nutrient solution and seedlings were incubated in this medium for 24 h at 250C, the 551 www.plantphysiol.org on May 12, 2018 -Published by Downloaded from

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Induction of Aluminum Tolerance in Wheat Seedlings by Low Doses of Aluminum in the Nutrient Solution

Aniol¹

1984

Plant Physiol.

122

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“…Weigel and Jager (1980) demonstrated that Cd in Phaseolus vulgaris seedlings was mainly bound to peptides and low molecular weight proteins. ln cabbage and tobacco leaves an inducible Cd binding protein of Me 10,000 was reported by Wagner and Trotter (1982), similarly almost all of the Cd in aqueous extracts of lycopersicon esculentum was recovered in association with an inducible protein fraction of Me 10,000 (Bartolf et al, 1980). Rauser (1984) isolated an inducible Cd binding polypeptide from Agrostis gigantea which contained over 90% of the ;oluble Cd.…”

Section: Discussionmentioning

confidence: 99%

“…However, it was not until 1980 that the existence of metal binding proteins was first reported in higher plants (Rauser and Curvetto, 1980). Subsequently, they have been found to occur in a number of plant species; for example, tomato (Lue-Kim and Rauser, 1986), soybean (Cataldo et al, 1981), wheat (Wagner and Nulty, 1984), tobacco and ~abbagc (Wagner and Trotter, 1982;Wagner and Yeargan, 1986). Grill et al (1985) isolated a group of Cd binding polypeptides from cell suspension cultures of more than a dozen different plant species and referred to these as phytochclatins.…”

Section: Introductionmentioning

confidence: 99%

Accumulated forms of thallium and cadmium inLemna minor. II. Relationship between duration of exposure and metal-protein binding

Kwan

Smith

1990

Chemical Speciation & Bioavailability

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Gel filtration chromatography (with Sephadex G25, G50 and G100) was used to separate the different forms of thallium (TI) and cadmium (Cd) in the cytosol fraction of Lemna minor and to examine the influence of the duration of metal exposure on the speciation of the two elements. A major proportion of Cd in the soluble phase was found to be bound to three groups of proteinaceous and polypeptide fractions; two of these, the high molecular weight protein fraction (Mr > 150,000) and the low polypeptide sized moieties of about Mr 1,SOO or less were constitutive entities, whereas the intermediate sized fraction (Mr 7,000-8,000) could only be detected in plants previously exposed to Cd. After 12 days of exposure to Cd this fraction accounted for the greatest part of the bound Cd in Lemna tissues. Extending the period of exposure from 18 hours to 12 days resulted in a shift in the distribution of Cd between the low and intermediate fractions. Evidence for Tl-protein binding was limited and confined to the high molecular weight fraction, and most of the Tl in the soluble phase was present in a form closely allied to the free ion. The contrasting behaviour between the two elements has been interpreted in terms of the differences in their physicochemical properties.

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“…Rechromatography of fraction B (Fig. 1) plants can be classified as a type of metallothionein which differs from that in animals (3)(4)(5)(6)(7)(8)(9). Characterization of this material in detail is in progress.…”

Section: Introductionmentioning

confidence: 99%

Chemical Form of Cadmium (and Other Heavy Metals) in Rice and Wheat Plants

Kaneta¹,

Hikichi²,

Endo³

et al. 1986

Environmental Health Perspectives

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Chemical forms of heavy metals such as Cd, Cu, Ni, and Pb in rice and wheat plants grown in nutrient solution containing a heavy metal were investigated. Fractionation of an extract of Cd-treated rice plants on Sephadex G-75 showed cadmium to be associated with organic compounds of high (fraction A), intermediary (fraction B), and low molecular weight (fraction C). Material A, whose molecular weight was greater than 440,000, is probably nonspecific binding of Cd to normal cell components. Materials B and C can be classified as types of metallothionein. The molecular weight of B was 33,100. This material contains 12 mg Cd/g protein. The UV-absorption spectrum of B showed absorptions at 280 and 250 nm. Material B was not eluted even at a very high ionic strength from the DEAE-cellulose column, but it was eluted at a very low ionic strength from a CM-cellulose column, indicating a highly anionic molecule which differs from metallothionein in animals. Fraction C contains two materials: one a Cd-containing material whose molecular weight was estimated to be approximately 7000 and the other an inorganic Cd salt. In addition to cadmium, copper, lead, and nickel in rice and wheat have been studied. As a result, heavy metal-containing materials whose molecular weights were estimated to be approximately 16,000 and 8900 (Ni-treated rice plants), 7000 (Pb-treated rice plants), 5000 (Cd-treated wheat plants), and 21,000 (Cu-treated wheat plants) were isolated.

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Inducible Cadmium Binding Complexes of Cabbage and Tobacco

Cited by 88 publications

References 14 publications

Induction of Aluminum Tolerance in Wheat Seedlings by Low Doses of Aluminum in the Nutrient Solution

Induction of Aluminum Tolerance in Wheat Seedlings by Low Doses of Aluminum in the Nutrient Solution

Accumulated forms of thallium and cadmium inLemna minor. II. Relationship between duration of exposure and metal-protein binding

Chemical Form of Cadmium (and Other Heavy Metals) in Rice and Wheat Plants

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