Inducible cellulase production from an organic solvent tolerant Bacillus sp. SV1 and evolutionary divergence of endoglucanase in different species of the genus Bacillus

Thomas, Lebin; Ram, Hari; Singh, V. P.

doi:10.1016/j.bjm.2017.05.010

Cited by 29 publications

(16 citation statements)

References 72 publications

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“…The aliquot of sample suspension 0.1 mL was spread on a Petri dish containing 1% CMC-agar medium and incubated at 30°C for 72 hours. The CMC-agar medium (g.L -1 ) consist of CMC 10, Yeast Extract 4, KH2PO4 4, Na2HPO4 4, MgSO4.7H2O 0.2, CaCl2.2H2O 0.001, FeSO4.7H2O 0.004, and agar 15 at pH 6 [11].…”

Section: Isolation and Screening Of Cellulolytic Bacteriamentioning

confidence: 99%

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Faizah

Ardyati

Suharjono

2020

JELS

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Palopo, South Sulawesi, is one of the traditional industrial centers of sago processing. The accumulation of sago pith waste around industrial sites can pollute the environment. Some microorganisms can degrade the cellulose in sago pith waste. This study was aimed to evaluate the indigenous cellulolytic bacteria from sago pith waste as a biodegradation agent. Bacteria were isolated from sago pith waste and grown on a 1% Carboxyl methyl cellulose (CMC) agar medium. The cellulolytic activity was analyzed semiquantitatively using 1% Congo red and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH variations of 4, 5, and 6. The potential isolate was identified based on 16S rDNA sequence similarity. This study obtained 21 bacterial isolates where six isolates were A1D, A1E, A1I, A1K, A2A, and B1A had the highest cellulolytic index at 0.82-1.13. Among those six isolates, the A1E isolate had the highest cellulolytic activity, 0.54 U.mL-1 at pH 6. The isolate A1E was identified as Burkholderia cepacia JCM 2799 with 99.73% similarity of 16S rDNA sequence.

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Section: Isolation and Screening Of Cellulolytic Bacteriamentioning

confidence: 99%

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Faizah

Ardyati

Suharjono

2020

JELS

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“…It is a promising method to decompose it by cellulase produced by microorganisms (Sukumaran et al 2005;Octave and Thomas 2009). This enzyme is widely used in textile industry, pharmaceutical industry, food industry, biofuel, pollution control, and feed industry (Sreena et al 2016;Aarti et al 2018;Thomas et al 2018;Sreena and Sebastian 2018). Keeping crop straw in the field can supplement soil organic matter and improve soil fertility.…”

Section: Introductionmentioning

confidence: 99%

Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency

et al. 2020

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Purpose The highly efficient degradation bacteria were selected from the humus from the very cold straw in China for many years to construct the in situ degradation bacteria, and the degradation efficiency of corn straw was determined by process optimization. Methods According to the main components of corn straw, through morphological, physiological, and biochemical screening, three highly efficient complementary degradation strains were selected to construct the compound flora, and the degradation efficiency was analyzed by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer. Result The corn straw selected in this paper is mainly composed of cellulose (31.99%), hemicellulose (25.33%), and lignin (14.67%). Through the determination of enzyme activity, strain Streptomyces sp. G1T has high decomposition ability to cellulose and hemicellulose but weak utilization ability to lignin; strain Streptomyces sp. G2T has the strongest decomposition ability to cellulose and hemicellulose among the three strains. The decomposition ability of strain Streptomyces sp. G3T to lignin was the strongest among the three strains. Therefore, by compounding the three strains, the decomposition ability has been greatly improved. The optimal process conditions obtained by single factor and response surface method are as follows: pH is 7, temperature is 30 °C, inoculation amount is 5%, rotational speed is 210 rpm, and the weight loss rate of straw is 60.55% after decomposing for 7 days. A large amount of degradation of corn straw can be seen by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer. Conclusion Streptomyces sp. G1T, Streptomyces sp. G2T, and Streptomyces sp. G3T screened from straw humus in very cold areas were used to construct in situ degradation bacteria, which had good straw degradation activity and had the potential to be used for straw treatment in cold areas after harvest. This characteristic makes the complex bacteria become a strong competitive candidate for industrial production, and it is also an effective biotechnology in line with the current recycling of resources.

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“…Among these protein sequences, most of the β-glucosidases were came from bacteria (282,519) (Chan et al 2016; Chen et al 2017; Sun et al 2018), followed by fungi (11,863) (Guo et al 2015; Hernandez-Guzman et al 2016). The β-glucosidases have the characteristics of cold adaptation (Crespim et al 2016; Ueda et al 2010), heat resistance (Leis et al 2018), salt tolerance (Lee et al 2015), glucose tolerance (Chamoli et al 2016; Chan et al 2016), and organic solvent tolerance (Thomas et al 2018) in extreme environments. β-Glucosidase is an indispensable component in the process of cellulose conversion to glucose (Sorensen et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification and molecular characterization of a psychrophilic GH1 β-glucosidase from the subtropical soil microorganism Exiguobacterium sp. GXG2

Yin

et al. 2019

AMB Expr

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The products of bacterial β-glucosidases with favorable cold-adapted properties have industrial applications. A psychrophilic β-glucosidase gene named bglG from subtropical soil microorganism Exiguobacterium sp. GXG2 was isolated and characterized by function-based screening strategy. Results of multiple alignments showed that the derived protein BglG shared 45.7% identities with reviewed β-glucosidases in the UniProtKB/Swiss-Prot database. Functional characterization of the β-glucosidase BglG indicated that BglG was a 468 aa protein with a molecular weight of 53.2 kDa. The BglG showed the highest activity in pH 7.0 at 35 °C and exhibited consistently high levels of activity within low temperatures ranging from 5 to 35 °C. The BglG appeared to be a psychrophilic enzyme. The values of Km, Vmax, kcat, and kcat/Km of recombinant BglG toward ρNPG were 1.1 mM, 1.4 µg/mL/min, 12.7 s−1, and 11.5 mM/s, respectively. The specific enzyme activity of BglG was 12.14 U/mg. The metal ion of Ca2+ and Fe3+ could stimulate the activity of BglG, whereas Mn2+ inhibited the activity. The cold-adapted β-glucosidase BglG displayed remarkable biochemical properties, making it a potential candidate for future industrial applications.

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Inducible cellulase production from an organic solvent tolerant Bacillus sp. SV1 and evolutionary divergence of endoglucanase in different species of the genus Bacillus

Cited by 29 publications

References 72 publications

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency

Identification and molecular characterization of a psychrophilic GH1 β-glucosidase from the subtropical soil microorganism Exiguobacterium sp. GXG2

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