2004
DOI: 10.1016/j.cub.2003.12.033
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Inducible Clustering of Membrane-Targeted SH3 Domains of the Adaptor Protein Nck Triggers Localized Actin Polymerization

Abstract: We show that high local concentrations of Nck SH3 domains are sufficient to stimulate localized, Cdc42-independent actin polymerization in living cells. This study provides strong evidence of a pivotal role for Nck in directly coupling ligand-induced tyrosine phosphorylation at the plasma membrane to localized changes in organization of the actin cytoskeleton through a signaling pathway that requires N-WASp.

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Cited by 101 publications
(85 citation statements)
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“…In line with this notion, we noticed a general decrease in the amount of polymerized actin in the KO cells. Targeted localization of Nck to specific foci at the plasma membrane has been shown to lead to a local assembly of the components in the actin polymerization machinery and a local actin polymerization (35). Nck has been shown to mediate in vitro actin polymerization together with phosphatidylinositol 4,5-bisphosphate, Rac1, and N-WASP (36).…”
Section: Discussionmentioning
confidence: 99%
“…In line with this notion, we noticed a general decrease in the amount of polymerized actin in the KO cells. Targeted localization of Nck to specific foci at the plasma membrane has been shown to lead to a local assembly of the components in the actin polymerization machinery and a local actin polymerization (35). Nck has been shown to mediate in vitro actin polymerization together with phosphatidylinositol 4,5-bisphosphate, Rac1, and N-WASP (36).…”
Section: Discussionmentioning
confidence: 99%
“…GST-tagged full-length Shp2 (GST-Shp2 FL) and GST-tagged Shp2 fragments containing the N-terminal sh2 domain, the C-terminal sh2 domain, and tandem sh2 (both N-and C-terminal sh2 domains) were subcloned with the full-length Shp2 construct as the template using standard PCR-based cloning techniques. The CD16-hemagglutinin (HA) construct was a gift from B. J. Mayer (University of Connecticut) (66). Constructs encoding fusion proteins consisting of the CD16 extracellular domain, CD7 transmembrane domain, and nephrin cytoplasmic domain and their mutants were generated using PCR-based techniques.…”
Section: Methodsmentioning
confidence: 99%
“…Mammalian plasmids encoding mouse Nephrin (30), Fyn (33), Fyn KD (K295M), and human Nephrin (gift from K. Tryggvason) (31) were described previously. The CD16-HA (containing an HA tag) construct was a gift from B. Mayer (University of Connecticut) (34). Constructs encoding fusion protein consisting of CD16 extracellular domain, CD7 transmembrane domain, and Nephrin cytoplasmic domain and their mutants were generated using PCR-based techniques and described previously (23,35).…”
Section: Methodsmentioning
confidence: 99%