Inducible P450s of the CYP9 family from larval Manduca sexta midgut

Stevens, Jennitte; Snyder, Mark J.; Koener, J. F.

doi:10.1016/s0965-1748(00)00024-2

Cited by 100 publications

(68 citation statements)

References 35 publications

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“…The CYP9 family has only recently been created, and the first member of this family, CYP9A1 from Heliothis virescens, has been associated with insecticide resistance (Rose et al 1997). Several isoforms of CYP9A from M. sexta have been identified and they are induced in the lepidoptera midgut by xenobiotics and allelochemicals (Stevens et al 2000).…”

Section: Genetic Mappingmentioning

confidence: 99%

A targeted approach to the identification of candidate genes determining susceptibility to Plasmodium gallinaceum in Aedes aegypti

et al. 2003

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The malaria parasite, Plasmodium, has evolved an intricate life cycle that includes stages specific to a mosquito vector and to the vertebrate host. The mosquito midgut represents the first barrier Plasmodium parasites encounter following their ingestion with a blood meal from an infected vertebrate. Elucidation of the molecular interaction between the parasite and the mosquito could help identify novel approaches to preventing parasite development and subsequent transmission to vertebrates. We have used an integrated Bulked Segregant Analysis-Differential Display (BSA-DD) approach to target genes expressed that are in the midgut and located within two genome regions involved in determining susceptibility to P. gallinaceum in the mosquito Aedes aegypti. A total of twenty-two genes were identified and characterized, including five genes with no homologues in public sequence databases. Eight of these genes were mapped genetically to intervals on chromosome 2 that contain two quantitative trait loci (QTLs) that determine susceptibility to infection by P. gallinaceum. Expression analysis revealed several expression patterns, and ten genes were specifically or preferentially expressed in the midgut of adult females. Real-time PCR quantification of expression with respect to the time of blood meal ingestion and infection status in mosquito strains permissive and refractory for malaria revealed a differential expression pattern for seven genes. These represent candidate genes that may influence the ability of the mosquito vector to support the development of Plasmodium parasites. Here we describe their isolation and discuss their putative roles in parasite-mosquito interactions and their use as potential targets in strategies designed to block transmission of malaria.

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Section: Genetic Mappingmentioning

confidence: 99%

A targeted approach to the identification of candidate genes determining susceptibility to Plasmodium gallinaceum in Aedes aegypti

et al. 2003

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“…Another instance occurs in Helicoverpa zea, the cotton bollworm; CYP6B8 is induced by xanthotoxin and the enzyme metabolizes the toxin (Li et al, 2003). In addition, several reports have suggested that some of insect P450s of the CYP4, CYP9, CYP28, and CYP6 families are induced by host plants, or participate in the detoxifi cation of the host-plant allelochemicals (Danielson et al, 1997David et al, 2006;Fogleman et al, 1998;Niu et al, 2008;Snyder et al, 1995;Stevens et al, 2000;Yang et al, 2007;Zhou et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Induction of Two Cytochrome P450 Genes, CYP6AE28 and CYP6AE30, in Cnaphalocrocis medinalis: Possible Involvement in Metabolism of Rice Allelochemicals

Liu

Yang

2010

Zeitschrift Für Naturforschung C

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Two cDNAs specific for P450 genes, CYP6AE28 and CYP6AE30, have been isolated from the rice leaf folder Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae). Both cDNApredicted proteins have 504 amino acid residues in length, but with molecular masses of 60177 Dalton for CYP6AE28 and 60020 Dalton for CYP6AE30, and theoretical pI values of 8.49 for CYP6AE28 and 8.56 for CYP6AE30, respectively. Both putative proteins contain the conserved structural and functional domains characteristic of all CYP6 members. CYP6AE28 and CYP6AE30 show 52% amino acid identity to each other; both of them have 49 - 56% identities with CYP6AE1, Cyp6ae12, and CYP6AE14. Phylogenetic analysis showed that the two P450s are grouped in the lineage containing some of the CYP6AE members, CYP6B P450s and CYP321A1. The transcripts of CYP6AE28 and CYP6AE30 were found to be induced in response to TKM-6, a rice variety with high resistance to C. medinalis. The results suggest that the two P450s may play important roles in adaptation to the host plant rice. This is the first report of P450 genes cloned in C. medinalis

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“…CYP28A transcripts and indole alkaloid metabolism are differentially induced in cactophilic Drosophila mettleri and D. nigrospiracula by senita and saguaro alkaloids in their drosophilid hostplants (Danielson et al, 1997;Fogleman et al, 1998). Collections of CYP4M and CYP9A transcripts are induced in Manduca sexta (tobacco hornworm) by aliphatic ketones (2-undecanone and 2-tridecanone) in their solanaceous hostplants (Snyder et al, 1995;Stevens et al, 2000). Direct evidence that these allelochemical-inducible P450s metabolize their respective inducers, however, has not yet been obtained.…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of CYP321A1, a novel cytochrome P450 involved in metabolism of plant allelochemicals (furanocoumarins) and insecticides (cypermethrin) in Helicoverpa zea

Sasabe

Wen

Berenbaum

et al. 2004

Gene

134

107

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Inducible P450s of the CYP9 family from larval Manduca sexta midgut

Cited by 100 publications

References 35 publications

A targeted approach to the identification of candidate genes determining susceptibility to Plasmodium gallinaceum in Aedes aegypti

A targeted approach to the identification of candidate genes determining susceptibility to Plasmodium gallinaceum in Aedes aegypti

Characterization and Induction of Two Cytochrome P450 Genes, CYP6AE28 and CYP6AE30, in Cnaphalocrocis medinalis: Possible Involvement in Metabolism of Rice Allelochemicals

Molecular analysis of CYP321A1, a novel cytochrome P450 involved in metabolism of plant allelochemicals (furanocoumarins) and insecticides (cypermethrin) in Helicoverpa zea

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