Inducing cells to disperse nickel nanowires via integrin-mediated responses

Sharma, Anirudh; Orlowski, Gregory M.; Zhu, Yuechen; Shore, Daniel; Kim, Seung‐Yeon; DiVito, Michael D.; Hubel, Allison; Stadler, Bethanie J. H.

doi:10.1088/0957-4484/26/13/135102

Cited by 34 publications

(34 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The core-shell NWs have already been used for different therapeutic applications such as cancer cell killing through different modalities including pH-triggered drug delivery, magneto-mechanic actuation [40], and more recently photothermal therapy, where the NWs showed an outstanding heating efficiency [43]. Functionalization with targeting agents allowed the selective interaction with leukemic cells [38], and these NWs show potential for hyperthermia treatment and magnetic guiding [75,76]. The possibility of using the NWs as MRI contrast agents opens the door for theranostics in diseases such as cancer, where different strategies can be combined for targeting (magnetic guiding and targeting agents) and for treatment, while following the process in a non-invasive manner.…”

Section: Magnetic Resonance Imaging Of Nanowires Internalized In Breamentioning

confidence: 99%

Magnetic core–shell nanowires as MRI contrast agents for cell tracking

et al. 2020

View full text Add to dashboard Cite

Background: Identifying the precise location of cells and their migration dynamics is of utmost importance for achieving the therapeutic potential of cells after implantation into a host. Magnetic resonance imaging is a suitable, non-invasive technique for cell monitoring when used in combination with contrast agents. Results: This work shows that nanowires with an iron core and an iron oxide shell are excellent materials for this application, due to their customizable magnetic properties and biocompatibility. The longitudinal and transverse magnetic relaxivities of the core-shell nanowires were evaluated at 1.5 T, revealing a high performance as T 2 contrast agents. Different levels of oxidation and various surface coatings were tested at 7 T. Their effects on the T 2 contrast were reflected in the tailored transverse relaxivities. Finally, the detection of nanowire-labeled breast cancer cells was demonstrated in T 2-weighted images of cells implanted in both, in vitro in tissue-mimicking phantoms and in vivo in mouse brain. Labeling the cells with a nanowire concentration of 0.8 μg of Fe/mL allowed the detection of 25 cells/µL in vitro, diminishing the possibility of side effects. This performance enabled an efficient labelling for high-resolution cell detection after in vivo implantation (~ 10 nanowire-labeled cells) over a minimum of 40 days. Conclusions: Iron-iron oxide core-shell nanowires enabled the efficient and longitudinal cellular detection through magnetic resonance imaging acting as T 2 contrast agents. Combined with the possibility of magnetic guidance as well as triggering of cellular responses, for instance by the recently discovered strong photothermal response, opens the door to new horizons in cell therapy and make iron-iron oxide core-shell nanowires a promising theranostic platform.

show abstract

Section: Magnetic Resonance Imaging Of Nanowires Internalized In Breamentioning

confidence: 99%

Magnetic core–shell nanowires as MRI contrast agents for cell tracking

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The use of magnetic nanostructures for biomedical applications has significantly increased in the last few years. Magnetic nanowires (NWs) have similar advantages as commonly used superparamagnetic iron oxide nanoparticles [1], when it comes to biocompatibility [2] and functionalization [3]. Their length and diameter can be easily modified, depending on the method of fabrication and the materials available [4].…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalizing Magnetic Nanowires Toward Targeting and Killing Leukemia Cancer Cells

et al. 2019

View full text Add to dashboard Cite

The objective of targeting technologies in the field of nanomedicine is to specifically focus therapies on diseased cells in order to deliver the treatment to these cells without harming healthy ones. The use of magnetic materials in such biomedical applications offers many advantages. They can be functionalized with an agent that enhances the biocompatibility and the targeting while, at the same time, they can be controlled and monitored remotely. Iron nanowires, which usually have a native oxide shell, are specifically attractive, since they are highly biocompatible, strongly magnetic and can be coated with different biological agents. Shape anisotropy makes these nanowires permanently magnetic and therefore can be exploited for multifaceted remote manipulations, rendering them versatile nano-robots. As such, they have been used before in combination with a magnetic field to induce cancer cell death. In order to minimize the side effects of this method this study aims to enhance the targeting ability of these nanowires toward particular cells. Specifically, leukemic cells are targeted by functionalizing iron nanowires with anti-CD44 antibodies, a cell surface marker, which is overexpressed in leukemic cells compared to healthy blood cells. Iron nanowires were electrochemically fabricated with an average diameter of 35 nm and a length around 3 μm. They were coated with bovine serum albumin to facilitate their conjugation covalently with an anti-CD44 antibody by using 3-(3-dimethyl-aminopropyl) carbodiimide and of N-hydroxysuccinimide. In order to confirm the presence of anti-CD44 antibodies on the surface of the nanowires, immunostaining, and Fourier transform infrared spectroscopy were used. In addition, cytotoxicity effects of bare iron nanowires, coated and functionalized nanowires were studied by using cell proliferation assays. These studies illustrated that the nanowires have a high level of biocompatibility.

show abstract

“…Sharma et al incubated Au-tipped Ni nanowires with osteosarcoma for our first look at internalization via endosomes [37], which was later confirmed with a more rigorous study that involved PEG and peptide (RGD) coatings [38]. These nanowires were found to have low cytotoxicity, first by acridine orange and propidium iodide dual staining [37] and later by IL-1 β , TNF- α , and MTS assays.…”

Section: Bio Applications: Flow Sensors Purification Barcodes Rmentioning

confidence: 98%

Galfenol Thin Films and Nanowires

Stadler

Reddy

Basantkumar

et al. 2018

Sensors

Self Cite

View full text Add to dashboard Cite

Galfenol (Fe1−xGax, 10 < x < 40) may be the only smart material that can be made by electrochemical deposition which enables thick film and nanowire structures. This article reviews the deposition, characterization, and applications of Galfenol thin films and nanowires. Galfenol films have been made by sputter deposition as well as by electrochemical deposition, which can be difficult due to the insolubility of gallium. However, a stable process has been developed, using citrate complexing, a rotating disk electrode, Cu seed layers, and pulsed deposition. Galfenol thin films and nanowires have been characterized for crystal structures and magnetostriction both by our group and by collaborators. Films and nanowires have been shown to be largely polycrystalline, with magnetostrictions that are on the same order of magnitude as textured bulk Galfenol. Electrodeposited Galfenol films were made with epitaxial texture on GaAs. Galfenol nanowires have been made by electrodeposition into anodic aluminum oxide templates using similar parameters defined for films. Segmented nanowires of Galfenol/Cu have been made to provide engineered magnetic properties. Applications of Galfenol and other magnetic nanowires include microfluidic sensors, magnetic separation, cellular radio-frequency identification (RFID) tags, magnetic resonance imaging (MRI) contrast, and hyperthermia.

show abstract

Inducing cells to disperse nickel nanowires via integrin-mediated responses

Cited by 34 publications

References 61 publications

Magnetic core–shell nanowires as MRI contrast agents for cell tracking

Magnetic core–shell nanowires as MRI contrast agents for cell tracking

Biofunctionalizing Magnetic Nanowires Toward Targeting and Killing Leukemia Cancer Cells

Galfenol Thin Films and Nanowires

Contact Info

Product

Resources

About