Inducing sporulation in Alternaria solani I. Effect of water treatment

Prasad, B. K.; Dutt, Bhupender; Nagaich, B. B.

doi:10.1007/bf02050856

Cited by 13 publications

(5 citation statements)

References 6 publications

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“…Surprisingly, the methodology (E) of this work showed that cold shock seems not to be effective in the sporulation. Such behavior is not in accordance with the work of Prasad et al, (17), wherein Alternaria solani showed sporulation under photoperiod of 12 hours and thermal shock at 4ºC. However the cultures of Prasad were dipped in cold water (4ºC) for 4 min only.…”

Section: Discussioncontrasting

confidence: 64%

Comparison of methodologies for conidia production by Alternaria alternata from citrus

Carvalho

Alves

Batista

et al. 2008

Braz. J. Microbiol.

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Conidia production is a problem in the study of Alternaria alternata from citrus. Thus, this study aimed to compare existing methodologies for conidial production of A. alternata isolated from Ponkan tangerine (2 isolates), Cravo lemon (1 isolate), Pêra orange (2 isolates) and Murcott tangor (1 isolate). The methodologies used were conidia production with 12 and 24 hours under white fluorescent light, evaluation with 24 and 48 hours after applying fungal mycelium stress technique, cold stress followed by injury of mycelium and evaluation with 24 hours, using healthy vegetable tissue and the use of black fluorescent near ultraviolet (NUV) lamp. Satisfactory result was obtained with A. alternata isolate from Murcott tangor, with the production of 2.8 x 10 5 conidia mL -1, when fungal mycelium was stressed (Petri dish with 66.66% of fungi growth) and subsequently 24 h of growth. The use of white light (24 h) and black fluorescent NUV lamp also induced expressive conidia production by one isolate of Ponkan tangerine, which produced 17.2 x 10 5 and 10.1 x 10 5 conidia mL -1 and another of Murcott tangor, which produced 13.9 x 10 5 and 10.1 x 10 5 conidia mL -1, respectively. The remaining methodologies analyzed in this study were not able to induce conidia production in satisfactory quantity. The use of both mycelium stress technique and white light (24 h) and black fluorescent NUV lamp allowed the production of enough quantities of conidia to be used in vitro (detection of fungitoxic substances) and in vivo (pathogenicity test) assays, respectively.

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Section: Discussioncontrasting

confidence: 64%

Comparison of methodologies for conidia production by Alternaria alternata from citrus

Carvalho

Alves

Batista

et al. 2008

Braz. J. Microbiol.

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“…Some workers, Rath and Padhi (1973), Gupta and Nikharaj (1972), Prasad et al (1973), Stevenson and Pennypacker (1988), Sodlauskiene (2003) and Rodriguez and Santana (1991); tested the isolate of A. solani on particular temperature for observing the cultural characters and sporulation of A. solani. Gemawat and Ghosh (1979) observed the effect of pH, vitamins and growth regulators for sporulation of A. solani.…”

Section: Sporulationmentioning

confidence: 98%

Cultural, morphological, pathogenic and molecular variability amongst tomato isolates of Alternaria solani in India

Kumar

Haldar

Pandey

et al. 2007

World J Microbiol Biotechnol

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Early blight (Alternaria solani) is an important disease causing severe damage in tomato. The eleven isolates of A. solani designated as So, Dh, Sh, Va-5, Ka, Ma, Hy, Ba-1, My, Va-3 and Mi were collected from different agroclimatic conditions and these isolates were characterized for cultural, morphological, pathogenic and molecular variations. The pigmentation varied from yellow, brown, black, brownish to greenish black in isolates of A. solani on potato dextrose agar medium. In general, radial growth of all isolates ranged between 14.9 mm and 32.2 mm on PDA and 24.3 mm to 53.7 mm on three selective media i.e., ASM, V-8 juice agar and V-8 juice agar (synthetic) on the fourth day. The fastest radial growth was recorded in the So isolate and slowest in the Ka isolate on PDA, while isolates Dh, Ba-1 and Va-3 were recorded to be faster in growth on ASM, V-8 juice agar and V-8 juice agar (synthetic) medium. The thickness of conidiogenous hyphae varied between 1.17 l and 9.56 l, with maximum in the Va-5 and Ma isolates. Most of the isolates showed smooth mycelial growth with circular and irregular margin and without concentric zonation. Sporulation was not found in any of the isolates on four different nutrient media, whereas conidiogenous hyphal length was observed in V-8 juice agar medium only. Based on the pathogenicity, isolates of A. solani were rated as virulent or less virulent based on percentage disease incidence data. Molecular variability studies were also done to find out the best annealing temperature and eighty-six primers were screened to select for maximum polymorphism of DNA. The best annealing temperature was recorded between 32.5°C and 34.0°C for the pathogen, and most efficient amplification and polymorphism of DNA was found with random primer 5 0 -CGCGTTCCTG-3 0 .

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“…The fungus was grown in PDA plate and incubated at 2472°C for four days. The spores were harvested from the fully grown cultures using cold distilled water spray as described by Prasad et al (1973) and the spore count was adjusted to 10 5 spores mL with distilled water using haemocytometer.…”

Section: Evaluation Of Antagonism Of Rhamnolipid Biosurfactant In Potmentioning

confidence: 99%

Characterization and antifungal properties of rhamnolipids produced by mangrove sediment bacterium Pseudomonas aeruginosa strain KVD-HM52

Deepika

Sridhar

Bramhachari

2015

Biocatalysis and Agricultural Biotechnology

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Inducing sporulation in Alternaria solani I. Effect of water treatment

Cited by 13 publications

References 6 publications

Comparison of methodologies for conidia production by Alternaria alternata from citrus

Comparison of methodologies for conidia production by Alternaria alternata from citrus

Cultural, morphological, pathogenic and molecular variability amongst tomato isolates of Alternaria solani in India

Characterization and antifungal properties of rhamnolipids produced by mangrove sediment bacterium Pseudomonas aeruginosa strain KVD-HM52

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