A research focusing on the education mode reform of colleges and universities in music teaching under 5G Internet is carried out to better apply 5G (5th generation mobile networks) Internet technology into the education. At first, advantages of 5G Internet are illustrated. Then it introduces characteristics of education informalization. Finally, it combines 5G Internet technology and education perfectly to design concrete scene of 5G in the educative application and conclude innovative application of 5G application in the education. Results suggest, on the basis of 5G Internet technology, education methods of colleges and universities in music teaching have been getting diversified. Especially on the current colleges and universities music class situation, colleges and universities music education puts music culture and music literacy together and deepens the verified exploration on education mode. Teachers on multimedia class extend diversified contents of music education. They gradually present different styles and ideas of music to student, which makes development of music education in broadness and depth and transforms traditional methods of music education. It will be a kind of joy for student to study it on class, which makes them consider more paths to study music. In this way, students can associate music with society development and add their music knowledge to more diversified music contents. In the meantime, application of music-thinking mode in multi-ethnic music learning, overall multi-culture music thinking, improvement of music literacy and enhancement of music culture soft power can be achieved.