Induction and characterisation of tetraploidy in Eucalyptus polybractea R.T. Baker

Fernando, S.C.; Goodger, Jason Q. D.; Chew, Bee Lynn; Cohen, Tal J.; Woodrow, Ian E.

doi:10.1016/j.indcrop.2019.111633

Cited by 16 publications

(15 citation statements)

References 39 publications

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“…Although no naturally occurring Eucalyptus polyploids have been found, artificial mutagenesis could give rise to them ( Guo et al, 2017 ). In previous studies on Eucalyptus tetraploid induction, only the use of colchicine to induce somatic chromosome doubling to obtain autotetraploids was reported, despite often obtaining a large number of mixoploids ( Fernando et al, 2019 ; Silva et al, 2019 ). Compared with inducing the chromosome doubling of somatic cells, the mutagenesis approach to obtain tetraploids by treating zygotic cells after fertilization clearly shows great advantages ( Lu et al, 2013 ; Guo et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

“…Although there have been characterizations of the tetraploid obtained by somatic chromosome doubling, the tetraploid obtained by zygotic chromosome doubling is unclear ( Ren et al, 2021 ). In Eucalyptus , it was reported that the growth of tree height and diameter at breast height of autotetraploids obtained by somatic chromosome doubling was slower than that of diploids, and their content of secondary metabolites such as essential oils showed no significant change compared with that of diploids ( Fernando et al, 2019 ). However, whether the variation trend in tetraploids obtained by zygotic doubling is consistent with that in those obtained by somatic doubling is the most important issue in breeding research and industrial production and applications.…”

Section: Introductionmentioning

confidence: 99%

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Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Liu

Wang²,

Qiu³

et al. 2022

Front. Plant Sci.

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Improvements in plant growth can bring great benefits to the forest industry. Eucalyptus urophylla is an important plantation species worldwide, and given that ploidy increases are often associated with plant phenotype changes, it was reasoned that its polyploidization may have good prospects and great significance toward its cultivation. In this study, the zygotic development period of E. urophylla was observed through paraffin sections, and a correlation between the development time of flower buds after pollination and the zygotic development period was established. On this basis, it was determined that the 25th day after pollination was the appropriate time for a high temperature to induce zygotic chromosome doubling. Then tetraploid E. urophylla was successfully obtained for the first time through zygotic chromosome doubling induced by high temperature, and the appropriate conditions were treating flower branches at 44°C for 6 h. The characterization of tetraploid E. urophylla was performed. Chromosome duplication brought about slower growing trees with thicker leaves, larger cells, higher net photosynthetic rates, and a higher content of certain secondary metabolites. Additionally, the molecular mechanisms for the variation in the tetraploid’s characteristics were studied. The qRT-PCR results showed that genes mediating the tetraploid characteristics showed the same change trend as those of the characteristics, which verified that tetraploid trait variation was mainly caused by gene expression changes. Furthermore, although the tetraploid had no growth advantage compared with the diploid, it can provide important germplasm resources for future breeding, especially for the creation of triploids.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Liu

Wang²,

Qiu³

et al. 2022

Front. Plant Sci.

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“…Analysing the morphology of diploid and tetraploid drumstick tree plants revealed typical features of polyploids. Polyploidy affects plant developmental rate and, frequently, the overall size of cells, organs, and the entire organism [ 1 , 27 , 31 , 32 ]. Chromosome doubling translates into doubling the number of genes, and this can increase the gene expression level associated with a particular beneficial feature [ 33 , 34 ].…”

Section: Resultsmentioning

confidence: 99%

In vitro induction and characterisation of tetraploid drumstick tree (Moringa oleifera Lam.)

et al. 2020

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Artificial induction of polyploidy is widely used in breeding programmes to improve the agronomic traits. The drumstick tree (Moringa oleifera Lam.) has a range of potential commercial uses, as the vegetative organs have high nutritional, medicinal, and feed values. In the present study, in vitro tetraploidisation in drumstick tree was performed by treating leaf segments with colchicine and subsequently verifying the ploidy levels. For polyploidisation, explant survival and regeneration rates were affected more by exposure time than by colchicine concentration, and the highest polyploidisation efficiency was observed at 500 mg/L colchicine for 3 days, which yielded 21% tetraploids. The morphological characteristics and contents of seven fodder-related nutrients (crude protein, ether extract, ash, acid detergent fibre, neutral detergent fibre, calcium, and phosphorus) in the leaves and shoots were compared between tetraploid and diploid drumstick trees. The resulting tetraploids showed significantly enhanced leaf and stomatal size. In addition, the contents of seven fodder-related nutrients were higher, although to varying degrees, in tetraploids than in diploids. The results indicated that the tetraploid produced in this study exhibited superior agronomical traits and improved biomass yield than diploids, and may represent excellent raw materials for fodder to enhance biomass and nutrition.

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“… Colchicine; 0.6%; 24 h Increase in total anthocyanin (9.1%) Soaking in colchicine soln. Colchicine; 0.6%; 24 h Increase in total phenolic (NM) Citrus limon (L.) Osbeck MS + colchicine; 25 ± 2 °C; 16 h PP; 50–60 μmol/m 2 /s LI Seed with radicle Colchicine; 0.025%; 24 h Diploid➔tetraploid (2n=4x=36) Increase in essential oil and limonene (7%) [ 58 ] Eucalyptus polybractea RT Baker Solid medium M1 + colchicine; Dark; 25 °C; shaking at 40 rpm Shoot tip Colchicine; 0.5%; 4 d Diploid➔tetraploid (2n=4x=44) Increase in essential oil (3%) [ 59 ] Plantago psyllium Colchicine treatment (dissolved in 2% DMSO) Terminal bud Colchicine; 0.5%; 24 h Diploid➔tetraploid (2n=4x=24) Increase in carotenoid (1.5-fold) [ 60 ] NM Trifluralin; 22.5%; 72 h Increase in carotenoid (1.54-fold) Plantago ovata Soaking in colchicine soln. Terminal bud Colchicine; 0.3%; 24 h Trifluralin; 22.5%; 72 h Diploid➔tetraploid (2n=4x=16) Increase in carotenoid content (approx.…”

Section: Introductionmentioning

confidence: 99%

Induced autopolyploidy—a promising approach for enhanced biosynthesis of plant secondary metabolites: an insight

Gantait

Mukherjee

2021

Journal of Genetic Engineering and Biotechnology

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Background Induced polyploidy serves as an efficient approach in extricating genetic potential of cells. During polyploidization, multiple sets of chromosomes are derived from the same organism resulting in the development of an autopolyploid. Alterations owing to artificially induced polyploidy level significantly influence internal homeostatic condition of resultant cells. Main text Induced autopolyploidy transpires as a result of an increase in the size of genome without any change in elementary genetic material. Such autopolyploidy, artificially induced via application of antimitotic agents, brings about a lot of beneficial changes in plants, coupled with very few detrimental effects. Induced autopolyploids exhibit superior adaptability, endurance to biotic and abiotic stresses, longer reproductive period and enzyme diversity coupled with enhanced rate of photosynthesis and gene action in comparison to their diploid counterparts. However, reduced rate of transpiration and growth, delay in flowering are some of the demerits of autopolyploids. Inspite of these slight unfavourable outcomes, induced autopolyploidization has been utilized in an array of instances wherein genetic improvement of plant species is concerned, since this technique usually boosts the biomass of concerned economic parts of a plant. In other way, it is also evident that multiplication of genome bestows enhanced production of secondary metabolites, which has contributed to a significant commercial value addition especially for plants with medicinal importance, in particular. Conclusion This review makes an attempt to explore the system and success of antimitotic agents vis-à-vis artificial autopolyploidization, interfered with the biosynthesis-cum-production of secondary metabolites having cutting-edge pharmaceutical importance.

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Induction and characterisation of tetraploidy in Eucalyptus polybractea R.T. Baker

Cited by 16 publications

References 39 publications

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

In vitro induction and characterisation of tetraploid drumstick tree (Moringa oleifera Lam.)

Induced autopolyploidy—a promising approach for enhanced biosynthesis of plant secondary metabolites: an insight

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