Induction and characterization of tetraploids in poplar

Yan, Xin; Zhang, Jie; Zhang, Hui

doi:10.1007/s11240-021-02043-0

Cited by 7 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In their study on Escallonia illinita and E. rubra, they observed that one of the indicators of polyploidy was the increase in leaf area. Yan, Zhang and Zhang (2021) claimed that polyploid poplar (Populus euroamericana) had characteristics of increasing in leaf, thicker and rounder leaves, with a larger size of stomata and more of chloroplasts, but a lower stomatal index on abaxial leaf surfaces.…”

Section: A Growth Responsementioning

confidence: 99%

THE EFFECTIVENESS OF COLCHICINE AND ORYZALIN ON POLYPLOIDY INDUCTION IN TEAK (Tectona grandis Linn. f.) IN VITRO

Fauzan,

Supriyanto,

Mulyono

et al. 2024

Ina.J.For.Res

View full text Add to dashboard Cite

Indonesian government has launched a community plantation forests program that would be beneficial for remedying shortage in domestic demand for teak wood every year. This program needs to be supported by the availability of quality seeds resources and quality seedlings utilizing polyploid teak (Tectona grandis). Our study aimed to examine the effectiveness of colchicine and oryzalin to modify diploid into polyploid T. grandis based on growth response, morphological, anatomical and cytological alteration, as well as the acclimatization ability of polyploid plantlets. The materials used were aseptic lateral shoots consisting of nodal segments which were immersed in antimitotic agents at the concentration of 0, 15 and 30 μM for 5 days, then cultured on regeneration medium until the 8 weeks and followed by acclimatization. The results showed that colchicine at the concentration of 30 μM was the most effective to induce polyploidy of plantlets in the parameter of high growth rate, the length of internodes, the number of leaf plantlets and increase the leaf surface area and the significant chlorophyll index content compared to the control. Anatomical analysis of polyploid was characterized by increasing the leaf thickness, stomata size, decreased stomatal density and increased chloroplast content in guard cells. Based on cytological examination on polyploidy plantlet, there was an increased in the number of chromosomes in the cell nucleus. The acclimatization of polyploid successfully induced rooting and a 100% survival rate of grown plantlets. Polyploid seedlings were able to grow and well adapted to the new environment of acclimatization

show abstract

Section: A Growth Responsementioning

confidence: 99%

THE EFFECTIVENESS OF COLCHICINE AND ORYZALIN ON POLYPLOIDY INDUCTION IN TEAK (Tectona grandis Linn. f.) IN VITRO

Fauzan,

Supriyanto,

Mulyono

et al. 2024

Ina.J.For.Res

View full text Add to dashboard Cite

show abstract

“…Woody perennials such as octaploid Ziziphus jujuba [ 14 ], Neolamarckia cadamba [ 15 ], Jatropha curcas [ 16 ], and Panicum virgatum L. [ 17 , 18 ]; and hexaploid Populus [ 19 , 20 ], Pennisetum [ 21 , 22 ], and Prunus pseudocerasus [ 23 ], as well as tetraploid Populus [ 24 – 26 ], Ziziphus jujuba [ 27 , 28 ], Robinia pseudoacacia [ 29 , 30 ], Platanus acerifolia [ 31 ], Pogostemon cablin [ 32 ], Liquidambar styraciflua [ 33 ], and Lagerstroemia indica [ 34 ] are examples of successful colchicine induction to achieve somatic chromosome doubling. Different explant types in vascular plants have been induced to obtain somatic chromosome doubling: the explant types include seeds [ 35 ], zygotes [ 36 – 38 ], shoot tips [ 16 , 30 , 39 ], nodal segments [ 15 , 20 ], leaf petioles [ 33 , 40 ], and leaf blades [ 24 , 28 ]. Hence, somatic chromosome doubling is a promising method to produce new polyploids.…”

Section: Introductionmentioning

confidence: 99%

In vitro induction of tetraploidy and its effects on phenotypic variations in Populus hopeiensis

Wu,

Zhou,

Sang

et al. 2023

BMC Plant Biol

View full text Add to dashboard Cite

Background Artificial induction of polyploidy is the most common and effective way to improve the biological properties of Populus and develop new varieties of this tree. In this study, in order to confirm and expand earlier findings, we established a protocol using colchicine and based on an efficient shoot regeneration system of leaf blades to induce tetraploidy in vitro in three genotypes from diploid Populus hopeiensis. The stomatal characteristics, leaf blade size, and growth were evaluated for diploids and tetraploids of three genotypes. Results We found that genotype, preculture duration, colchicine concentration, and colchicine exposure time had highly significant effects on the tetraploid induction rate. The optimal protocol for inducing tetraploidy in P. hopeiensis was to preculture leaf blades for 7 days and then treat them for 4 days with 40 mg/L colchicine. The tetraploid induction rates of genotypes BT1, BT3, and BT8 were 21.2, 11.4 and 16.7%, respectively. A total of 136 tetraploids were identified by flow cytometry analysis and somatic chromosome counting. The stomatal length, width, and density of leaf blades significantly differed between diploid and tetraploid plants. Compared with their diploid counterparts, the tetraploids produced larger leaf blades and had a slower growth rate. Our findings further document the modified morphological characteristics of P. hopeiensis following whole-genome duplication (e.g., induced tetraploidy). Conclusions We established a protocol for in vitro induction of tetraploidy from diploid leaf blades treated with colchicine, which can be applied to different genotypes of P. hopeiensis.

show abstract

“…Tetraploid plants can be distinguished from diploid plants by more chloroplasts and a darker green color [12,13]. The tetraploid Populus had significantly longer and wider stomata and lower stomatal density than the diploid [14][15][16][17]. In Eucalyptus urophylla, chromosome duplication resulted in slower-growing trees with a higher net photosynthetic rate, more giant cells, thicker leaves, and a higher concentration of specific secondary metabolites [18].…”

Section: Introductionmentioning

confidence: 99%

Production of Tetraploid Plants from Cotyledons of Diploid Melia volkensii Gürke

et al. 2023

View full text Add to dashboard Cite

Polyploidy was induced in Melia volkensii (Mukau), a valuable native tree from the semi-arid regions of East Africa. Cotyledons of diploid M. volkensii (2n = 2x = 28) were treated with oryzalin for 0 (control), 1, 2, or 3 h with or without pretreatment with 1.1 µM thidiazuron. Cotyledons treated with 10 mg·L−1 oryzalin for three hours yielded 40% tetraploids. Pretreatment of cotyledons with thidiazuron for 18 days followed by treatment with oryzalin increased tetraploid plant production to 52.5%, but this also yielded more mixoploids. Compared to diploid M. volkensii, the tetraploid in vitro and young potted plants were compacter, with thicker stems, wider leaves, and a low density of longer and wider stomata. In the coming years, tetraploid M. volkensii plants will be observed in field trials and serve as a basis for further breeding efforts.

show abstract

Induction and characterization of tetraploids in poplar

Cited by 7 publications

References 16 publications

THE EFFECTIVENESS OF COLCHICINE AND ORYZALIN ON POLYPLOIDY INDUCTION IN TEAK (Tectona grandis Linn. f.) IN VITRO

THE EFFECTIVENESS OF COLCHICINE AND ORYZALIN ON POLYPLOIDY INDUCTION IN TEAK (Tectona grandis Linn. f.) IN VITRO

In vitro induction of tetraploidy and its effects on phenotypic variations in Populus hopeiensis

Production of Tetraploid Plants from Cotyledons of Diploid Melia volkensii Gürke

Contact Info

Product

Resources

About