Induction and cultures of mountain ginseng adventitious roots and AFLP analysis for identifying mountain ginseng

Son, Sung Ho; Choi, Sung Mee; Hyung, Soo Jin; Yun, Seung Rho; Choi, Myung Suk; Shin, Eun-Myoung; Hong, Young Pyo

doi:10.1007/bf02932381

Cited by 21 publications

(9 citation statements)

References 15 publications

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“…IBA was thus favorable for induction of the adventitious root in P. notoginseng culture. This is partially supported by similar studies on Panax ginseng, where IBA was also effective (Son et al 1999, Hahn et al 2003, Seo et al 2003.…”

Section: Effect Of Exogenous Phytohormones On Adventitious Root Initisupporting

confidence: 63%

Induction and Characterization of Adventitious Roots Directly from the Explants of Panax notoginseng

et al. 2005

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Adventitious roots from leafstalks and lateral roots were obtained directly from explants of Panax notoginseng. The lateral root explants were more sensitive to the induction of adventitious roots using indole-3-butyric acid. HPLC analysis of saponins extracted from the adventitious roots indicated that several protopanaxatriol saponins were present but ginsenoside Rd was missing, compared with the saponins extracted from the raw herbs. The dry weight of primary adventitious root culture of Panax notoginseng increased 5.25 times during multiplication in a classical shaking-flask system, suggesting that it is a culture system with great potential for scale-up.

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Section: Effect Of Exogenous Phytohormones On Adventitious Root Initisupporting

confidence: 63%

Induction and Characterization of Adventitious Roots Directly from the Explants of Panax notoginseng

et al. 2005

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“…Traditional cultivation methods cannot meet the increasing demands of the ginseng market, especially for ginsenosides. We recently established a bioreactor culture system for ginseng adventitious root cultures (Seon et al, 1999;Son et al, 1999a). However, the ginsenoside content in ginseng adventitious roots is not very high.…”

Section: Introductionmentioning

confidence: 99%

Improvement of ginsenoside production by jasmonic acid and some other elicitors in hairy root culture of ginseng (Panax ginseng C. A. Meyer)

Gao

Son³

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

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Hairy root cultures of Panax ginseng, established after the infection of root sections with Agrobacterium rhizogenes KCTC 2703, were cultured in phytohormone-free Murashige and Skoog (MS) liquid medium containing different concentrations of jasmonic acid and some other elicitors, in order to promote ginsenoside accumulation. Jasmonic acid in the range 1.0±5.0 mg l 21 (4.8±23.8 mM) strongly improved total ginsenoside production in ginseng hairy roots. Peptone (300 mg l 21 ) also showed some effect on ginsenoside improvement; however its effect was much weaker than that of jasmonic acid. Ginsenoside content and productivity were 58.65 and 504.39 mg g 21 , respectively. The Rb group of ginsenoside content was increased remarkably by jasmonic acid, while Rg group ginsenoside content changed only slightly compared to controls. However, jasmonic acid also strongly inhibited ginseng hairy root growth.

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“…Induction and growth of hairylike adventitious roots is achieved from initial root explants by exogenous auxin supply, which is direct motive for the mass production of ginseng roots for commercial scale. Son et al [71] designed a balloon-type bubble bioreactor (BTBB) (Figures 1, 2A), which is superior for biomass growth than the bubble column bioreactor, and stirred tank bioreactor in cell culture of Taxus cuspidata [72], Beta vulgaris hairy roots [73], ginseng hairy root [74] and adventitious root culture [75]. The fresh weight of ginseng hairy-like adventitious root culture in 20-L BTBB was three-times higher than that of the stirred tank bioreactor [71].…”

Section: Commercial Production Of Panax Ginseng Roots Via Balloon Typmentioning

confidence: 97%

“…Son et al [71] designed a balloon-type bubble bioreactor (BTBB) (Figures 1, 2A), which is superior for biomass growth than the bubble column bioreactor, and stirred tank bioreactor in cell culture of Taxus cuspidata [72], Beta vulgaris hairy roots [73], ginseng hairy root [74] and adventitious root culture [75]. The fresh weight of ginseng hairy-like adventitious root culture in 20-L BTBB was three-times higher than that of the stirred tank bioreactor [71]. The maximum biomass production of 2.2 kg fresh weight in 20-L bioreactor was obtained after 42 days after inoculation of 240 g [76].…”

Section: Commercial Production Of Panax Ginseng Roots Via Balloon Typmentioning

confidence: 97%

“…However, hairy roots are still not well utilized for the production of health food and need further analysis for the safety of proteins and compounds expressed by introduced genes of T-DNA. Recently, hairy-like adventitious roots culture without transformation with Agrobacterium rhizogenes was reported [70,71]. Induction and growth of hairylike adventitious roots is achieved from initial root explants by exogenous auxin supply, which is direct motive for the mass production of ginseng roots for commercial scale.…”

Section: Commercial Production Of Panax Ginseng Roots Via Balloon Typmentioning

confidence: 99%

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Types And Designs Of Bioreactors For Hairy Root Culture

Choi

Kim

Paek

Plan Tissue Culture Engineering

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Induction and cultures of mountain ginseng adventitious roots and AFLP analysis for identifying mountain ginseng

Cited by 21 publications

References 15 publications

Induction and Characterization of Adventitious Roots Directly from the Explants of Panax notoginseng

Induction and Characterization of Adventitious Roots Directly from the Explants of Panax notoginseng

Improvement of ginsenoside production by jasmonic acid and some other elicitors in hairy root culture of ginseng (Panax ginseng C. A. Meyer)

Types And Designs Of Bioreactors For Hairy Root Culture

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