Induction and detection of long-lasting peptide-specific antibody responses in pigs and beef cattle: a powerful technology for tracing meat processing chains from stock farmers to sales counters

Raschke, Annett; Strich, Sandra; Huppke, Stephanie; Neugebauer, Markus; Geuther, Eugen; Bertling, Wolf; Walders, Birgit; Reiser, Christian O. A.; Hess, Jürgen

doi:10.1016/j.foodcont.2004.09.004

Cited by 8 publications

(7 citation statements)

References 17 publications

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“…At present, the ImmunoTrack® technology is limited to tracking M. hypopneumoniae in swine and cattle by means of an antibody response to an undisclosed KLH-conjugated peptide. [3][4][5] The responsif GMBH results clearly demonstrate that the positive vaccination marker approach is safe and effective in animal populations. There is no technical reason that positive vaccination markers cannot therefore be extended rapidly and with confidence to human vaccines such as BCG as well as to animal vaccines such as FMD and AI.…”

mentioning

confidence: 90%

Positive Vaccination Markers

Root‐Bernstein

2007

Human Vaccines

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mentioning

confidence: 90%

Positive Vaccination Markers

Root‐Bernstein

2007

Human Vaccines

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“…These antipeptide antibodies were easy to detect in meat, in organs such as the liver and in the blood of animals by utilization of peptide-specific ELISA. Our field studies revealed (i) the induction of longlasting antipeptide immunoglobulin (Ig) G antibody responses in pigs and cattle, (ii) that antipeptide IgG antibodies are detectable in serum and meat via ELISA, and (iii) a robust antipeptide antibody detection in meat juice, which is reproducible after storage of meat at room temperature, 4°C and −20°C [5].…”

Section: Surveillance Technologies In Livestock Husbandry and Meat Prmentioning

confidence: 93%

“…In our marker formulations the adjuvants QuilA or Montanide ® IMS1313 for cattle or pigs, respectively, worked best [4][5][6].…”

Section: Surveillance Technologies In Livestock Husbandry and Meat Prmentioning

confidence: 99%

“…Administration of the KLH/peptide compounds with presence of nonspecific immunostimulatory adjuvants applied either as a single intramuscular (i.m.) or subcutaneous (s.c.) injection or as prime/boost immunizations induced strong, peptide-specific antibody responses in pigs and cattle [5]. These antipeptide antibodies were easy to detect in meat, in organs such as the liver and in the blood of animals by utilization of peptide-specific ELISA.…”

Section: Surveillance Technologies In Livestock Husbandry and Meat Prmentioning

confidence: 99%

“…In this review we would like to focus on the potential of the ImmunoTrack technology concerning established animal health structures and improved transparency for meat consumers (Fig. 1) (Neugebauer et al, unpublished results) [4][5][6]. Furthermore, existing information systems enabling animal identification, and the underlying EC regulations, are outlined.…”

Section: Introductionmentioning

confidence: 99%

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ImmunoTrack®: The novel antibody‐based technology for tracing in animal health

Ruehland¹,

Reichel²,

Neugebauer³

et al. 2006

Biotechnology Journal

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This paper describes a novel antibody-based livestock movement control tool and method of meat allocation, both in livestock husbandry as well as during the meat-processing chain. Immuno Track fulfills diverse prerequisites and meets regulatory demands which are substantial for a successful monitoring technology: (i) the induction of long-lasting antibody responses detectable onsite throughout the whole mast period of pigs, (ii) a single immunization injection with protein derivatives is sufficient to evoke a strong epitope-specific antibody response, and (iii) the complete degradation of the protein markers after the antibody response has been triggered in meatproducing animals such as cattle or pigs. There are diverse fields of application for the Immuno-Track marker technology, such as in quality meat programs, as compliance markers for animal vaccines or as a tool for verification of origin. Combination of this monitoring technology with the husbandry and identification databases for cattle and pigs within the European Community will lead to greater transparency in meat production, thereby regaining consumers' trust in concomitant structures of the meat-producing industry.

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Development of a vaccine marker technology: Display of B cell epitopes on the surface of recombinant polyomavirus‐like pentamers and capsoids induces peptide‐specific antibodies in piglets after vaccination

Neugebauer¹,

Walders²,

Brinkman³

et al. 2006

Biotechnology Journal

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Highly immunogenic capsomers (pentamers) and virus-like particles (VLPs) were generated through insertion of foreign B cell epitopes into the surface-exposed loops of the VP1 protein of murine polyomavirus and via heterologous expression of the recombinant fusion proteins in E. coli. Usually, complex proteins like the keyhole limpet hemocyanin (KLH) act as standard carrier devices for the display of such immunogenic peptides after chemical linkage. Here, a comparative analysis revealed that antibody responses raised against the carrier entities, KLH or VP1 pentamers, did not significantly differ up to 18 weeks, demonstrating the highly immunogenic nature of VP1-based particulate structures. The carrier-specific antibody response was reproducibly detected in the meat juice after processing. More importantly, chimeric VP1 pentamers and VLPs carrying peptides of 12 and 14 amino acids in length, inserted into the BC2 loop, induced a strong and long-lasting humoral immune response against VP1 and the inserted foreign epitope. Remarkably, the epitope-specific antibody response was only moderately decreased when VP1 pentamers were used instead of VLPs. In conclusion, we identified polyomavirus VP1-based structures displaying surface-exposed immunodominant B cell epitopes as being an efficient carrier system for the induction of potent peptide-specific antibodies. The application of this approach in vaccine marker technology in livestock holding and the meat production chain is discussed.

show abstract

Induction and detection of long-lasting peptide-specific antibody responses in pigs and beef cattle: a powerful technology for tracing meat processing chains from stock farmers to sales counters

Cited by 8 publications

References 17 publications

Positive Vaccination Markers

Positive Vaccination Markers

ImmunoTrack®: The novel antibody‐based technology for tracing in animal health

Development of a vaccine marker technology: Display of B cell epitopes on the surface of recombinant polyomavirus‐like pentamers and capsoids induces peptide‐specific antibodies in piglets after vaccination

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