Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M

Uchiyama, Hiroo

doi:10.1016/s0378-1097(98)00536-9

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…Microorganisms that are effective at degrading pollutants can be stressed by exposure to these chemicals, as has been observed for Methylocystis sp. M and trichloroethylene [11], Acinetobacter calcoaceticus and phenol [12], Pseudomonas sp. B4 and polychlorinated biphenyls [13], and Pseudomonas putida KT2440 and toluene [14].…”

Section: Introductionmentioning

confidence: 99%

Adaptations in microbiological populations exposed to dinitrophenol and other chemical stressors

Ray

Peters

2010

Enviro Toxic and Chemistry

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Microbiological populations in natural and engineered systems may experience multiple exposures to chemical stressors, which may affect system functions. The impact of such exposures on the metabolism of a population of Pseudomonas aeruginosa was studied using respirometry. Two serial exposures to low concentrations of 2,4-dinitrophenol (DNP), pentachlorophenol (PCP), or N-ethyl maleimide (NEM) did not affect metabolism beyond that expected for a single exposure. However, at higher concentrations, three exposures to DNP led to a combination of metabolic stress and resilience in the population. At a low DNP concentration of 400 mg/L, multiple exposures led to increased stress but indicated no development of resilience. At a high DNP concentration of 1,200 mg/L, no biological activity was observed, indicating that the population did not survive the exposure. At intermediate concentrations of 800 and 900 mg/L DNP, stress was observed, but it was found to decrease after multiple exposures. This, combined with the observation that the size of the population decreased, indicated that resilience in the population had developed because of elimination of the weaker organisms in the population. In contrast, the lack of resilience at the lower DNP concentration was attributed to the survival of the strong as well as weak members, lowering the resilience of the population as a whole. The development of resilience within a window of stressor concentrations is an important finding with implications for predicting the performance of biotreatment processes and biosensor technologies and for interpreting ecotoxicity risk assessments.

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Section: Introductionmentioning

confidence: 99%

Adaptations in microbiological populations exposed to dinitrophenol and other chemical stressors

Ray

Peters

2010

Enviro Toxic and Chemistry

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“…The induction of stress reponses caused by aromatic phenolic compounds has been shown in Pseudomonas putida (Lupi et al ., 1995), Methylocystis sp. (Uchiyama et al ., 1999), Burkholderia sp. (Cho et al ., 2000), Acinetobacter radioresistens (Giuffrida et al ., 2001), Acinetobacter calcoaceticus (Benndorf et al ., 2001), Acinetobacter lwoffii (Kim et al ., 2004), Stenotrophomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis

Tam

Eymann

Albrecht

et al. 2006

Environmental Microbiology

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Aromatic organic compounds that are present in the environment can have toxic effects or provide carbon sources for bacteria. We report here the global response of Bacillus subtilis 168 to phenol and catechol using proteome and transcriptome analyses. Phenol induced the HrcA, sigmaB and CtsR heat-shock regulons as well as the Spx disulfide stress regulon. Catechol caused the activation of the HrcA and CtsR heat-shock regulons and a thiol-specific oxidative stress response involving the Spx, PerR and FurR regulons but no induction of the sigmaB regulon. The most surprising result was that several catabolite-controlled genes are derepressed by catechol, even if glucose is taken up under these conditions. This derepression of the carbon catabolite control was dependent on the glucose concentration in the medium, as glucose excess increased the derepression of the CcpA-dependent lichenin utilization licBCAH operon and the ribose metabolism rbsRKDACB operon by catechol. Growth and viability experiments with catechol as sole carbon source suggested that B. subtilis is not able to utilize catechol as a carbon-energy source. In addition, the microarray results revealed the very strong induction of the yfiDE operon by catechol of which the yfiE gene shares similarities to glyoxalases/bleomycin resistance proteins/extradiol dioxygenases. Using recombinant His6-YfiE(Bs) we demonstrate that YfiE shows catechol-2,3-dioxygenase activity in the presence of catechol as the metabolite 2-hydroxymuconic semialdehyde was measured. Furthermore, both genes of the yfiDE operon are essential for the growth and viability of B. subtilis in the presence of catechol. Thus, our studies revealed that the catechol-2,3-dioxygenase YfiE is the key enzyme of a meta cleavage pathway in B. subtilis involved in the catabolism of catechol.

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“…The induced heat shock proteins, oxidative stress proteins and so far unidentified proteins contribute to the bacteria resistance by stabilizing the cell functions as well as by diminishing the levels of toxic compounds and metabolites. The discovery of further proteins specifically induced by chemicals or specific unfavorable growth conditions could enable the development of better tools to evaluate the effects of xenobiotics on detoxifying bacteria [9,19].…”

Section: Discussionmentioning

confidence: 99%

“…Our understanding of the contribution made by stress proteins to resistance against xenobiotics has been advanced by several recent studies on bacteria like Pseudomonas putida [8], Methylocystic sp. [9], and Burkholderia sp. [10] that are able to metabolize these compounds.…”

Section: Introductionmentioning

confidence: 99%

Protein synthesis patterns inAcinetobacter calcoaceticusinduced by phenol and catechol show specificities of responses to chemostress

Benndorf

Loffhagen

Babel

2001

FEMS Microbiology Letters

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The proteins induced in Acinetobacter calcoaceticus by the potentially toxic growth substrates phenol and catechol were analyzed by 2D-electrophoresis of cell extracts and compared with those induced by heat shock and oxidative stress. Although both aromatic compounds are quite similar, the only difference being that catechol has an additional hydroxyl group, the responses obtained differed considerably. Phenol has greater lipophilicity and mainly induced heat shock proteins, whereas catechol, which causes the production of reactive oxygen species, predominantly induced oxidative stress proteins. Furthermore, some special proteins were induced by phenol or catechol, which might be useful as biomarkers for chemostress, and could be involved in the catalytic degradation of potentially toxic compounds.

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Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M

Cited by 4 publications

References 15 publications

Adaptations in microbiological populations exposed to dinitrophenol and other chemical stressors

Adaptations in microbiological populations exposed to dinitrophenol and other chemical stressors

Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis

Protein synthesis patterns inAcinetobacter calcoaceticusinduced by phenol and catechol show specificities of responses to chemostress

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