Induction and Growth of ‘Microspore-Derived’ Embryos of<i>Brassica napus</i>ssp.<i>oleifera</i>

Dunwell, J. M.; Cornish, M A; Courcel, A. G. L. De; Middlefell-Williams, Jill

doi:10.1093/jxb/34.12.1768

Cited by 33 publications

(14 citation statements)

References 6 publications

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“…Other profound cytoplasmic, nuclear rearrangements, or changes in gene expression occur before and during the induction of microspore embryogenesis (Bárány et al 2005;Testillano et al 2005;Seguí-Simarro & Nuez 2008). Even if it is commonly known that thermal treatments are the most effective and easiest methods to induce pollen embryogenesis, the optimum level and duration of the shock depends on the genotype (Dunwell et al 1983). In this study, different results were produced by the same thermal shock.…”

Section: Resultsmentioning

confidence: 99%

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Cardoso

Abdelgalel

Chiancone

et al. 2015

Plant Biosystems - An International Journal Dealing with all As

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In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the genotype: both clementines, namely Hernandina and Corsica, produced homozygous and triploid regenerants (microspore-derived embryos), whereas all of the analyzed regenerants from sweet oranges, three cultivars of Tarocco and Moro, produced heterozygous and diploid regenerants similar to the parental genotypes (somatic embryos).

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Section: Resultsmentioning

confidence: 99%

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Cardoso

Abdelgalel

Chiancone

et al. 2015

Plant Biosystems - An International Journal Dealing with all As

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“…The beneficial role of temperature shocks on the induction of pollen-embryos is established [8,9]. The coldtreatment slows down the regular development of pollen and prevents their degeneration,which further depends on the temperature, duration of the cold treatment and varies wibh bho genotypes.…”

Section: Resultsmentioning

confidence: 99%

Pollen-embryogenesis and chromosomal variability in anther culture of Brassica hirta Moench (Sinapis alba L)

Yps¹,

Mohaptra²

1990

Cell Res

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The anther cultures of Brassica hirta underwent poIlenembryogenesis and callusing, which showed a wide range of chromosome numbers varying from 9 (n=12) to a highly polyploid. For embryogenesis, pretreatment of floral buds in 0.4M sucrose solution for 72 hrs at 4℃ was superior to freshly cultured anthers. Culture temperature of 30℃ for 14 days before maintenance of cultures at 25℃ was significantly beneficial for embryo yield in comparison to cultures conlinuously incubated at 25℃ . Dark treatment during culture was more effective for pollen-embryo yield.

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“…It may disrupt the normal integrated development of the somatic anther tissue and subsequently it may synchronize the physiological states of the two tissues and thereby stimulate the induction process of true embryos (Dunwell et al 1983).…”

Section: Pseudo-embryos Derived From Anther Wall Cellsmentioning

confidence: 99%

Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide

Bal¹,

Shariatpanahi²,

Castro³

et al. 2012

Czech J. Genet. Plant Breed.

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This review describes sources of structures of non-microspore origin observed in anther and microspore cultures. Various characteristics of these structures may cause a wrong diagnosis of these structures as embryos or cell/tissue clusters of microspore origin. Here we suggest such structures to be named as pseudoembryogenic structures. The introduction of pseudo-embryogenic structures and their origins could be helpful to distinguish them from true microspore-derived structures. Prompted by certain environmental cues, somatic cells existing as a contamination in immature pollen (microspores) cultures can lead to the formation of 'pseudo-embryos' commonly known as embryoids. The pseudo-embryogenic structures may be classified in the following groups: (i) pseudo-star-like structures; pseudo-multicellular structures; (ii) pseudo-embryos with pseudo-suspensors; (iii) contaminating bacteria appearing as callus colonies; (iv) calli and embryos of somatic origin; (v) giant tetrad-like structures; (vi) anther wall cells. The exact origin of these structures is discussed in this paper, and some recommendations are proposed in order to avoid misinterpretation.

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Induction and Growth of ‘Microspore-Derived’ Embryos ofBrassica napusssp.oleifera

Cited by 33 publications

References 6 publications

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Pollen-embryogenesis and chromosomal variability in anther culture of Brassica hirta Moench (Sinapis alba L)

Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide

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