Induction and identification of Stylosanthes guianensis tetraploids

Wu, Fanhua; Yu, Xuebiao; Zhuang, Nansheng; Liu, G D; Liu, J P

doi:10.4238/2015.october.19.13

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…This research found that P. Ox Black Face Ox 1647 had the highest number of orchid buds compared to the other two orchid varieties (Table 1). A similar study by [18] showed colchicine treatment on young shoots of Lilium flowers of the Con. Amore variety with a concentration of 2,000 ppm caused 81.3% flower loss.…”

Section: Quantitative Characteristics Of Flowersupporting

confidence: 52%

Morphology of hybrid orchid induced by polyploidy using colchicine

Hartati

Samanhudi

Cahyono

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Phalaenopsis orchid flower is one of the essential aspects that has received significant attention from orchid breeders and farmers. Undesirable floral characters can be improved and enhanced by polyploidization using chemically induced antimitotic compounds such as colchicine. This reserach aimed to determine the morphological characteristics of orchids after being colchicine. This research was conducted on December 2021-February 2022 in Screen House, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta. In this research, three orchids of different types were used, that is P. Ox Black Face Ox 1647, P. Ox Spot Queen Ox Rouge Ox 1460, and P. Chain Xen Moment. This experimental study uses three samples of orchids given colchicine and orchids without colchicine. Each sample was repeated three times. The treatment was carried out by dripping colchicine 1,250 ppm on the buds of the three varieties, then covered with cotton and carbon paper. This study used descriptive analysis with data collection techniques, including direct observation and morphological documentation of three varieties of orchids. The results of this research indicated the success of P. Ox Spot Queen Ox Rouge Ox 1460, which had larger flower widths and lengths, that is, 9 cm long and 20 cm wide, and had an average flower autumn time of 60 days longer than the other two varieties.

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Section: Quantitative Characteristics Of Flowersupporting

confidence: 52%

Morphology of hybrid orchid induced by polyploidy using colchicine

Hartati

Samanhudi

Cahyono

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…The basic chromosome number of the hyacinth cultivar was eight, and the numbers of chromosomes in the diploid, triploid, tetraploid, and aneuploid cultivars were 16, 23, 24, 31, and 32, respectively. In a similar study, Wu et al (2015) studied the induction and identification of Stylosanthes guianensis tetraploids. Souza et al (2015) observed the genetic alteration and meiotic behavior of Brachiaria decumbens hybrids.…”

Section: Discussionmentioning

confidence: 99%

Mutagenic influences of colchicine on phenological and molecular diversity of Calendula officinalis L.

El-Nashar¹,

Ammar²

2016

Genet. Mol. Res.

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ABSTRACT. Six different colchicine concentrations: 0, 400, 800, 1200, 1600, and 2000 ppm, in combination with four soaking time treatments (1, 2, 3, and 4 h), were selected to assess the effects on germination, vegetative growth, and flower yield components in calendula plants. The molecular diversity among the treatments was assessed using ten SRAP marker combinations. Seed soaking in colchicine significantly enhanced both the fresh and the dry shoot and root masses, flowering date, number of flowers per plant, and flower diameter. At 1200-ppm colchicine combined with a 4-h soaking time, a superior effect on seed germination was observed, whereas 800 ppm for 4 h produced the highest number of flowers and the largest flower diameter. The earliest flowering time was found at 800 ppm combined with a short soaking time (1 h), while the 4-h soaking time with 800 ppm, is recommended for growing calendula outdoors, since it enhances flower development. At the molecular level, 752 fragments were successfully amplified

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“…Tanaman stylo (Stylosanthes guianensis) adalah tanaman leguminosa asli dari Amerika Selatan dan Tengah (Wu et al 2015) yang memiliki tinggi berkisar 0,6 m-1,2 m dengan diameter batangnya 0,3 cm-0,8cm (Jiang et al 2017) panjang daun mencapai 6 cm, memiliki kandungan protein kasar yang cukup tinggi jika dibandingkan dengan rumput yaitu berkisar pada 16%-17%, kandungan bahan kering 15%-23% serta produksi biomassa pertahunnya mencapai 40 ton ha -1 berdasarkan bahan kering (Litbang Pertanian,2012;Yulia et al 2022). Stylo merupakan salah satu tanaman leguminosa yang sudah banyak dimanfaatkan sebagai pakan ternak karena memiliki daya adaptasi yang bagus khususnya pada kondisi asam.…”

Section: Pendahuluanunclassified

Efektivitas Fungi Mikoriza Arbuskula yang Diproduksi dengan Teknik Fortifikasi Nutrisi Berbeda terhadap Produktivitas Stylosanthes guianensis pada Cekaman Kekeringan

Marzukah,

Manuhara Karti,

Prihantoro

2023

JINTP

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This study aimed to test the effectiveness of arbuscular mycorrhizal fungi (AMF) products against stylo plants (Stylosanthes guianensis) under drought stress conditions. The research design used a factorial complete random pattern (CRD) with 2 factors. Factor A was AMF: F0 (control), FU (superior AMF as a comparison), FIPB1 (1000ppm ABmix nutrient fortification technique), FIPB2 (2000ppm ABmix nutrient fortification technique), FIPB3 (3000ppm ABmix nutrient fortification technique). Factor B was the drought level, 100% field capacity (FC), 60% FC (medium dry level), and 40% FC (high dry level). The fertilizer for control plants (without AMF) was 100% dose, while plants with AMF were given a 50% dose, adding AMF ± 20 grams. The results showed an interaction between the drought factor and the different types of AMF. AMF was effective in symbiosis with stylo plants at all levels of drought. FIPB1 and FIPB2 could increase plant adaptation up to 60% FC. All AMF inoculations effectively increased leaf chlorophyll content at all drought levels. Biomass accumulation in FU and FIPB1 plants produced the best biomass. The research concluded that the quality of AMF produced with different nutrient fortifications were as good as the superior AMF (FU). Inoculation of AMF products (IPB1, IPB2, and IPB3) with 50% fertilization effectively increased the adaptation and production of Stylosanthes guianensis to a drought level of 60% FC compared to control (without AMF, 100% fertilization). Key words: Arbuscular mycorrhizal fungi, drought level, productivity, Stylosanthes guianensis

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Induction and identification of Stylosanthes guianensis tetraploids

Cited by 6 publications

References 17 publications

Morphology of hybrid orchid induced by polyploidy using colchicine

Morphology of hybrid orchid induced by polyploidy using colchicine

Mutagenic influences of colchicine on phenological and molecular diversity of Calendula officinalis L.

Efektivitas Fungi Mikoriza Arbuskula yang Diproduksi dengan Teknik Fortifikasi Nutrisi Berbeda terhadap Produktivitas Stylosanthes guianensis pada Cekaman Kekeringan

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