Induction and inhibition of pinocytosis by aminoglycoside antibiotics

Johansson, Pia; Josefsson, J.‐O.; Nässberger, Lennart

doi:10.1111/j.1476-5381.1984.tb16215.x

Cited by 11 publications

(1 citation statement)

References 24 publications

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“…It is of interest to ask why the uptake of gentamicin in the newborn kidney cortex is less than in adults. Aminoglycosides are known to be potent inducers of pinocytosis (Johansson et al 1984), which agrees with the finding that gentamicin is taken up into the kidney cortex of adult rats by pinocytosis (Silverblatt & Kuehn 1979). In general, biochemical processes in the developing kidney may be affected by the rapid growth of this organ.…”

Section: Discussionsupporting

confidence: 83%

Uptake of Gentamicin in Foetal and Newborn Rats after a Single Intraperitoneal Injection

Nässberger

DePierre

1989

Pharmacology & Toxicology

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The aim of the present investigation has been to examine 1) the placental passage and uptake of gentamicin in foetal rat tissues and 2) uptake and organ distribution of this drug in neonatal rats with special reference to the kidney. After a single intraperitoneal injection of tritium-labelled gentamicin to the mother, we found a rapid passage of radioactivity across the rat placenta, but no pronounced tendency to accumulation in the foetus. After administration of 10 micrograms or 100 mg gentamicin per kg body weight to pregnant rats at day 11 or 13, respectively, of gestation, the maximal concentrations of the drug recovered in individual foetuses were 0.05 and 300 ng per mg wet weight. Ten and 90 min. after administration the uptake of gentamicin in the kidney cortex of the mother was 25 and 118 fold higher, respectively, than the corresponding uptake in the foetal kidney after 19 days of gestation. After a single intraperitoneal injection, gentamicin uptake in the kidney cortex of neonatal rats resembled that seen in adult animals, with a rapid initial phase followed by a relatively stable plateau, although the maximal uptake was only about 10% as large in the neonates.

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Section: Discussionsupporting

confidence: 83%

Uptake of Gentamicin in Foetal and Newborn Rats after a Single Intraperitoneal Injection

Nässberger

DePierre

1989

Pharmacology & Toxicology

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Transport and Metabolism in the Hepatobiliary System

Meijer

1989

Comprehensive Physiology

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Effects of neomycin on calcium channel currents in clonal GH3 pituitary cells

Suarez‐Kurtz

Reuben

1987

Pflugers Arch.

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Membrane Ca currents were recorded from voltage-clamped clonal (GH3) pituitary cells under conditions where currents through Na and K channels were abolished. Two Ca currents, with distinct kinetics and voltage dependence for activation and inactivation, were identified. Neomycin, an aminoglycoside polycation, inhibited both the "transient" (ICa,t) and the "slowly-inactivating" (ICa,s) Ca currents in a dose-dependent manner (100-1,000 microM). The blockade was reversible and ICa,s was more sensitive to neomycin than ICa,t. The inhibition of ICa,s was frequency and time-independent, and was not affected by changes in the holding membrane potential (-35 to -100 mV). Neomycin did not affect the voltage dependence for inactivation of ICa,t. The blockade of both Ca currents by neomycin is ascribed to the general property of aminoglycosides to compete with, and displace Ca ions from membrane binding sites that determine the currents and selectivity of Ca channels. Because comparable concentrations of neomycin were required for blocking the currents conveyed by Ca or, in the absence of external divalent cations, by Na ions through the slowly-inactivating Ca channels, we suggest that the neomycin binding sites are distinct from the high-affinity transition sites within the Ca channel path.

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Induction and inhibition of pinocytosis by aminoglycoside antibiotics

Cited by 11 publications

References 24 publications

Uptake of Gentamicin in Foetal and Newborn Rats after a Single Intraperitoneal Injection

Uptake of Gentamicin in Foetal and Newborn Rats after a Single Intraperitoneal Injection

Transport and Metabolism in the Hepatobiliary System

Effects of neomycin on calcium channel currents in clonal GH3 pituitary cells

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