Induction and Isolation of Vascular Cells From Human Induced Pluripotent Stem Cells—Brief Report

Abstract

Objective-Induced pluripotent stem (iPS) cells are a novel stem cell population derived from human adult somatic cells through reprogramming using a defined set of transcription factors. Our aim was to determine the features of the directed differentiation of human iPS cells into vascular endothelial cells (ECs) and mural cells (MCs), and to compare that process with human embryonic stem (hES) cells. Methods and Results-We previously established a system for differentiating hES cells into vascular cells. We applied this system to human iPS cells and examined their directed differentiation. After differentiation, TRA1-60 Ϫ Flk1 ϩ cells emerged and divided into VE-cadherin-positive and -negative populations. The former were also positive for CD34, CD31, and eNOS and were consistent with ECs. The latter differentiated into MCs, which expressed smooth muscle ␣-actin and calponin after further differentiation. The efficiency of the differentiation was comparable to that of human ES cells. Conclusions-We succeeded in inducing and isolating human vascular cells from iPS cells and indicate that the properties of human iPS cell differentiation into vascular cells are nearly identical to those of hES cells. This work will contribute to our understanding of human vascular differentiation/development and to the development of vascular regenerative medicine.

Pluripotent Stem Cell-Engineered Cell Sheets Reassembled with Defined Cardiovascular Populations Ameliorate Reduction in Infarct Heart Function Through Cardiomyocyte-Mediated Neovascularization

“…We further established a more efficient CM induction as well as purification methods in human iPSCs [61]. EC and MC induction from human iPSCs has already been reported [62]. Technological basis to generate human cardiac tissue sheets from iPSCs has thus been established.…”

Cell-Based Vascularization Strategies for Skin Tissue Engineering

“…À fraction gives rise to vascular structures in vitro, [119][120][121] but has never been used for vasculogenic purposes in vivo so far. Although iPSC are not entirely similar to ESC, they also carry the risk of teratoma formation and genetic instability.…”

Mesenchymal Stem Cells: Possibilities of New Treatment Options

“…Moreover, cell fate specifi cation and maturation can be selectively altered via manipulation of endogenous developmental signaling pathways (Rathjen and Rathjen 2003 ;Meyer et al 2009 ) . The ability to differentiate iPS cells in vitro to specifi c lineages effi ciently and reproducibly has been achieved using those described protocols with certain modifi cations (Dimos et al 2008 ;Narazaki et al 2008 ;Tateishi et al 2008 ;Wernig et al 2008 ;Ebert et al 2009 ;Hu and Zhang 2009 ;Jin et al 2009 ;Karumbayaram et al 2009 ;Pfannkuche et al 2009 ;Senju et al 2009 ;Tanaka et al 2009 ;Taura et al 2009b ;Zhang et al 2009b ;Carvajal-Vergara et al 2010 ;Comyn et al 2010 ;Dick et al 2010 ;Gamm and Meyer 2010 ;Huang et al 2010 ;Kaichi et al 2010 ;Lamba et al 2010 ;Lee et al 2010 ;Martinez-Fernandez et al 2010 ;Parameswaran et al 2010 ;Swistowski et al 2010 ;Teramura et al 2010 ;Zhou et al 2010 ) .…”

“…Examples are auditory retinal cells (Jin et al 2009 ;Comyn et al 2010 ;Lamba et al 2010 ;Parameswaran et al 2010 ) , cardiomyocytes (Narazaki et al 2008 ;Pfannkuche et al 2009 ;Tanaka et al 2009 ;Zhang et al 2009b ;CarvajalVergara et al 2010 ;Kaichi et al 2010 ;Martinez-Fernandez et al 2010 ) , insulinsecreting islet-like clusters (Tateishi et al 2008 ) , motor neurons (Dimos et al 2008 ;Ebert et al 2009 ;Hu and Zhang 2009 ;Karumbayaram et al 2009 ) , dopaminergic neurons (Wernig et al 2008 ;Cai et al 2010 ;Swistowski et al 2010 ) , auditory spinal ganglion neurons (Nishimura et al 2009 ) , smooth muscle cells (Taura et al 2009b ;Xie et al 2009 ) , vascular endothelial cells (Taura et al 2009b ) , dendritic cells and macrophages (Senju et al 2009(Senju et al , 2010 , adipocytes (Tashiro et al 2009 ;Taura et al 2009a ) , osteoblasts (Tashiro et al 2009 ) , hematopoietic cells (Hanna et al 2007 ;Eminli et al 2009 ;Kaufman 2009 ;Lu et al 2009 ;Okabe et al 2009 ;Raya et al 2009 ;Kaneko et al 2010 ) , and endothelial progenitor cells (Xu et al 2009 ;Abaci et al 2010 ;…”