Induction by ethylene of cyanide-resistant respiration

Solomos, T.; Laties, George G.

doi:10.1016/0006-291x(76)91098-6

Cited by 57 publications

(36 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We used this concept by employing cyanide to examine whether the upsurge in respiration and peroxides reflect the activation of a similar path or are a function of the trigger compound employed. Figure 2A shows that the application of HCN in air induced a respiratory upsurge, as previously observed (16), and that the rise in respiration was accompanied by an increase in peroxides (17,18) that ethylene triggers the cyanide-insensitive respiration. Hamilton (13) argued that the single most important reason for the low kinetic reactivity of 02 is that molecular 02 exists in the ground triplet state.…”

supporting

confidence: 80%

“…Likewise, ethylene and propylene in 02 induced higher levels of peroxides (Fig. ID) these and other results, these authors proposed that ethylene stimulates an alternate respiratory pathway which is also triggered by cyanide and other respiratory poisons (17,18). We used this concept by employing cyanide to examine whether the upsurge in respiration and peroxides reflect the activation of a similar path or are a function of the trigger compound employed.…”

mentioning

confidence: 73%

See 1 more Smart Citation

Upsurge in Respiration and Peroxide Formation in Potato Tubers as Influenced by Ethylene, Propylene, and Cyanide

Chin

Frenkél²

1977

Plant Physiol.

View full text Add to dashboard Cite

A continuous application of ethylene (10 tlVI) and propylene (500 1d/l) to potato tubers (Solanum tuberosum L.) resulted in an upsurge of respiration and a concomitant rise in peroxides. When applied in 100% 02, the effect of ethylene and propylene on respiration and peroxide formation was augmented. Hydrogen cyanide (500 1d/1) mimicked the action of ethylene and propylene inducing a respiratory rise and a corresponding increase in peroxides. As with ethylene, the effect of HCN was augmented in high 02 tensions. The results support the suggestion that ethylene activates the cyanide-insensitive respiratory pathway.Previous studies showed that applied ethylene stimulates peroxide formation in ripening pears (3), suggesting that the action of ethylene consists, in part, of inducing peroxide formation in tissues. However, in climacteric fruit, including pear (3) and tomato (8), both ethylene and peroxides increase concomitantly, and for that reason it is difficult to ascertain the interrelation between the compounds.Application of ethylene to potato tubers induces a respiratory upsurge (14) resembling the rise in respiration displayed by climacteric fruit (2). However, the respiratory rise in potatoes, unlike fruit, can be studied as related directly to ethylene action since it is not spontaneous, and clearly depends on the application of ethylene (14). In addition, the ethylene-induced respiratory rise in potatoes can be studied as an independent senescence process. For that reason, we employed potato tubers in order to study the action of ethylene as it pertains to the stimulation of respiration and the changes in peroxides.In the present work, we show that the respiratory upsurge induced by ethylene and its analogue, propylene, is accompanied by a similar upsurge in the formation of peroxides. High 02 tensions enhanced respiration and the corresponding rise in the formed peroxides. Cyanide mimicked the action of ethylene. MATERIALS AND METHODSLocally grown potato tubers (Solanum tuberosum L. var. Northchip) were preconditioned at room temperature for 2 weeks following harvest. Whole potato tubers, each weighing approximately 150 g, were placed in 4-liter jars and ventilated continuously with different gas mixtures, at a flow rate of 400 ml/min. The employed gas mixtures consisted of air (21% 02) and 100% 02. Each 02 regime was supplemented with either zero, 10 ,lI ethylene, 500 ,ul propylene, or 500 ,ul cyanide/I ventilating gas, as previously described (9). The ambient temperature was maintained at 21 C for the duration of the experiment.Samples consisting of 1 kg on the average were used for the measurement of C02 evolution (10) and peroxide levels as previously described using a titanium reagent (3). These measurements were performed at intervals for 36 hr. All determinations were run in duplicate.RESULTS AND DISCUSSION Figure 1 shows the effect of ethylene and propylene on CO2 evolution by potatoes tubers (lA) and the corresponding changes in peroxide levels (1C) as related to time of treatment. Potat...

show abstract

supporting

confidence: 80%

mentioning

confidence: 73%

Upsurge in Respiration and Peroxide Formation in Potato Tubers as Influenced by Ethylene, Propylene, and Cyanide

Chin

Frenkél²

1977

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Solomos and Laties (15) have noted that there is a relationship between the effects of ethylene on respiration and the presence of the alternative pathway. Preliminary experiments showed that substantial amounts of ethylene were produced during the respiratory burst.…”

Section: Discussionmentioning

confidence: 99%

“…Studies with corn seedlings indicated that respiration was uncoupled at the peak of this respiratory burst, since DNP2 had no effect on the rates of 02 uptake (3). This uncoupled respiration could be a consequence of alternative respiratory pathway activity, which branches at ubiquinone, thus bypassing two of three phosphorylation sites of the Cyt-linked respiratory chain (6,7,14,15). This can result in thermogenesis (5, 11,14), and it may also permit a greater turnover of glycolysis and the Krebs cycle before the feedback inhibitory level of ATP is reached.…”

mentioning

confidence: 99%

Temperature Effects on the Activity of the Alternative Respiratory Pathway in Chill-Sensitive Cucumis sativus

Kiener¹,

Bramlage²

1981

Plant Physiol.

View full text Add to dashboard Cite

After 48 hours at 2°C, hypocotyls from chili-sensitive Cucumis sativus s ins showed a burst of 02 uptake. The alternative pathway became engaged to close to 45% fill capacity during this postchilling respiratory burst. However, it only accounted for up to 50% of this increased respiratory 02 uptake. By 24 hours after chiling, when the seedlngs were fully recovered from visible symptoms of chilling 4ury, the flux through the alteative pathway was back to the level (about 10%) found before chilng.Blocking chilling-induced ethylene production with aminoethoxyvinylgycine had no effect on this increased utilization of the alternative pathway.The direct effects of temperature on respiration rates and the effects of inhibitors suggested that there was a rapid increase in altermative pathway activity and decrease in the cytochrome pathway activity. The possibility that the alternative pathway represents a compensatory mechanism for the more labile cytochrome pathway is discussed.Chill-sensitive plants that have been exposed to low temperatures exhibit a burst of respiration, as measured by 02 uptake, after they are returned to nonchilling temperature (4, 9). This accelerated respiration might be associated with recovery from chilling damage. However, before any role can be assigned, it must be determined how the plant accomplishes this respiratory rise. Studies with corn seedlings indicated that respiration was uncoupled at the peak of this respiratory burst, since DNP2 had no effect on the rates of 02 uptake (3). This uncoupled respiration could be a consequence of alternative respiratory pathway activity, which branches at ubiquinone, thus bypassing two of three phosphorylation sites of the Cyt-linked respiratory chain (6,7,14,15). This can result in thermogenesis (5,11,14), and it may also permit a greater turnover of glycolysis and the Krebs cycle before the feedback inhibitory level of ATP is reached. In this study, the possible involvement of the alternative pathway in the postchilling burst of cucumber seedling respiration was investigated. The postchilling burst of 02 uptake in cucumber seedlings is sensitive to SHAM (12). This burst coincided with the appearance of the chief chilling-injury symptom of these seedlings, i.e. collapse with a flaccid appearance. The results described represent a more detailed analysis of this system showing the relative contributions of the Cyt and alternative pathway using the titration method devised by Bahr and Bonner (1). MATERIALS AND METHODSCucumber seedlings (Cucumis sativus L. cv. Marketmore 70) were used throughout this study. Seeds were imbibed in water for 2 h at 22°C and sown on vermiculite. They were then grown for 8 days at 27 ± 1°C in continuous light (160 mE/cm2. s) at a RH of 80 ± 5%. Seedlings were then either transferred to 2 ± 1GC, 95% RH in the dark or kept at 27°C but in the dark. This was to eliminate any photooxidation effects of chilling in the light. In some experiments, AVG was used to control ethylene production. VT' was then plotted against g(i...

show abstract

“…The presence of a functional alternative oxidase was evidenced by insensitivity of 02 uptake to the presence of cyanide or AA3 [classical inhibitors of the Cyt electron transport pathway (10)] and specific inhibition by substituted hydroxamic acids, including SHAM (13). Cyanide-insensitive 02 uptake has been measured in a variety of plant preparations ranging from differential and gradient centrifugation fractions (2,3,8,15) to tissue slices (9) and whole seeds (5 tive respiration in view of shortcomings inherent to inhibitor studies.…”

mentioning

confidence: 99%

Cyanide-insensitive and Cyanide-sensitive O₂ Uptake in Wheat

Goldstein¹,

Anderson²,

McDaniel³

1981

Plant Physiol.

View full text Add to dashboard Cite

Induction by ethylene of cyanide-resistant respiration

Cited by 57 publications

References 13 publications

Upsurge in Respiration and Peroxide Formation in Potato Tubers as Influenced by Ethylene, Propylene, and Cyanide

Upsurge in Respiration and Peroxide Formation in Potato Tubers as Influenced by Ethylene, Propylene, and Cyanide

Temperature Effects on the Activity of the Alternative Respiratory Pathway in Chill-Sensitive Cucumis sativus

Cyanide-insensitive and Cyanide-sensitive O₂ Uptake in Wheat

Contact Info

Product

Resources

About

Induction by ethylene of cyanide-resistant respiration

Cited by 57 publications

References 13 publications

Upsurge in Respiration and Peroxide Formation in Potato Tubers as Influenced by Ethylene, Propylene, and Cyanide

Upsurge in Respiration and Peroxide Formation in Potato Tubers as Influenced by Ethylene, Propylene, and Cyanide

Temperature Effects on the Activity of the Alternative Respiratory Pathway in Chill-Sensitive Cucumis sativus

Cyanide-insensitive and Cyanide-sensitive O2 Uptake in Wheat

Contact Info

Product

Resources

About

Cyanide-insensitive and Cyanide-sensitive O₂ Uptake in Wheat