Induction of 6‐thioguanine‐resistant mutants in human diploid fibroblasts in vitro with ethylene oxide

Kolman, Ada; Bohušová, T.; Lambert, Bo; Simons, J.W.I.M.

doi:10.1002/em.2850190203

Cited by 18 publications

(2 citation statements)

References 16 publications

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“…The titer and ratio of these factors in the nuclei of brain cells may dictate the profile of genes that are expressed, for example, during reproductive and nonreproductive seasons. Changes in transcription factor concentrations as small as 2-fold to 3-fold have been shown to result in differential regulation of target genes (Struhl et al, 1989;Kolman et al, 1992). In the present study we did not determine relative levels of HasPOU-III and HasPOU-IV transcripts or proteins.…”

Section: Pou Gene Expression and Evolutionmentioning

confidence: 60%

Expression of POU, Sox, and Pax Genes in the Brain Ganglia of the Tropical Abalone Haliotis asinina

O‘Brien

Degnan

2000

Marine Biotechnology

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In gastropod mollusks, neuroendocrine cells in the anterior ganglia have been shown to regulate growth and reproduction. As a first step toward understanding the molecular mechanisms underlying the regulation of these physiological processes in the tropical abalone Haliotis asinina, we have identified sets of POU, Sox, and Pax transcription factor genes that are expressed in these ganglia. Using highly degenerate oligonucleotide primers designed to anneal to conserved codons in each of these gene families, we have amplified by reverse transcriptase polymerase chain reaction 2 POU genes (HasPOU-III and HasPOU-IV), 2 Sox genes (HasSox-B and HasSox-C), and two Pax genes (HasPax-258 and HasPax-6). Analyses with gene-specific primers indicated that the 6 genes are expressed in the cerebral and pleuropedal ganglia of both reproductively active and spent adults, in a number of sensory structures, and in a subset of other adult tissues.

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Section: Pou Gene Expression and Evolutionmentioning

confidence: 60%

Expression of POU, Sox, and Pax Genes in the Brain Ganglia of the Tropical Abalone Haliotis asinina

O‘Brien

Degnan

2000

Marine Biotechnology

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“…Experiments were conducted using human skin fibroblasts (VH10) [46] , immortalised by the human telomerase reverse transcriptase (hTERT) method [47] and supplemented with G418 (25 μg/ml final, AppliChem) for selection. For the investigation of the role of SAGA DUB, HeLa cells (malignant human epithelial adenocarcinoma cells) [48] transfected and stably expressing short hairpin RNA against ATXN7L3 (shATXN7L3) and non-targeting control (shCTL) were used [35] , kindly provided to us by the Tora Lab, IGBMC.…”

Section: Methodsmentioning

confidence: 99%

Histone H2Bub dynamics in the 5′ region of active genes are tightly linked to the UV-induced transcriptional response

Fanourgakis

Synacheri

Lavigne

et al. 2023

Computational and Structural Biotechnology Journal

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Propylene oxide and epichlorohydrin induce DNA strand breaks in human diploid fibroblasts

Kolman

Spivak

Näslund

et al. 1997

Environ. Mol. Mutagen.

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The induction of DNA strand breaks in human diploid fibroblasts (VH‐10) was demonstrated after in vitro exposure with two carcinogenic epoxides, propylene oxide (PO) and epichlorohydrin (ECH). Alkaline DNA unwinding (ADU), pulsed field gel electrophoresis (PFGE), and the camel assay were used to measure DNA single‐ (SSBs) and double‐strand breaks (DSBs). A dose‐dependent increase of DNA strand breaks, measured by ADU, was observed in the dose range 2.5–2.0 mMh of PO and 0.25‐2 mMh of ECH. The dose‐response of ECH was about five times higher compared with that of PO (211 vs. 41 SSBs · 100 Mbp−1 · mMh−1). The induction rates of DSBs, measured by PFGE, were found to be 18 times higher for ECH compared to PO (4.8 and 0.27 DSBs · 100 Mbp−1 · mMh−1 for ECH and PO, respectively). Using these two methods, the SSBs/DSBs ratio was estimated to be 148 for PO and 44 for ECH. The data obtained by the camel assay also demonstrated a dose‐dependent ability of PO and ECH to induce DNA damage. It was found that ECH was about six times more effective as an inducer of DNA strand breaks compared to PO (200 and 32 · 100 Mbp−1 · mMh−1 for ECH and PO, respectively). The SSBs/DSBs ratios calculated using comet assay and PFGE data were 125 for ECH and 41 for PO. In addition, ECH is about 10 times more toxic than PO with respect to survival. These properties of ECH can at least in part be explained by its higher chemical reactivity connected with a higher rate of DNA alkylation. Environ. Mol. Mutagen. 30:40–46, 1997 © 1997 Wiley‐Liss, Inc.

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Induction of 6‐thioguanine‐resistant mutants in human diploid fibroblasts in vitro with ethylene oxide

Cited by 18 publications

References 16 publications

Expression of POU, Sox, and Pax Genes in the Brain Ganglia of the Tropical Abalone Haliotis asinina

Expression of POU, Sox, and Pax Genes in the Brain Ganglia of the Tropical Abalone Haliotis asinina

Histone H2Bub dynamics in the 5′ region of active genes are tightly linked to the UV-induced transcriptional response

Propylene oxide and epichlorohydrin induce DNA strand breaks in human diploid fibroblasts

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