Induction of a bystander effect after therapeutic ultrasound exposure in human melanoma: In-vitro assay

Rezaei, Mahnaz; Samani, Roghayeh Kamran; Kazemi, Mohammad; Shanei, Ahmad; Hejazi, Seyed Hossein

doi:10.29252/ijrr.19.1.183

Cited by 5 publications

(2 citation statements)

References 21 publications

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“…However, Hao et al reported a viability value of 62% for C6 glioma cells upon UW exposure at 1.0 W cm -2 and 1.0 MHz for one minute [ 38 ]. Another study showed that the A375 cell-killing ability of UW depended on the time of exposure [ 39 ]. It can be decided that the biocompatibility of UW depends on the cell type, and exposure conditions of time, intensity, and frequency [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

A Study of Sonodynamic Therapy of Melanoma C540 Cells in Vitro by Titania/Gold Nanoparticles

2023

J Biomed Phys Eng

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Background: Sonodynamic Therapy (SDT), a safe and non-invasive strategy in tumor therapy, is in development using novel sono-sensitizers, activated by low-intensity ultrasound radiation. SDT mainly progresses through Reactive Oxygen Species (ROS) generation followed by cell annihilation. Objective: The current study aimed to investigate the effect of ultrasound therapy with titania/gold nanoparticles (NPs) on melanoma cancer. Material and Methods: In this experimental study, Titania/gold NPs (TGNPs) were synthesized, and their activity was investigated in sonodynamic therapy of a melanoma cancer cell line (C540). SDT was performed at 1.0 W cm -2 and 1.0 MHz for one minute. Results: The synthesized NPs that comprised gold NPs of <10 nm into titania NPs of <20 nm showed great stability and cytocompatibility. While TGNPs were biocompatible, a remarkable rate of cell ablation was observed upon ultrasound irradiation due to ROS generation. Conclusion: The SDT using TGNPs can be introduced as an alternative and low-cost treatment method for melanoma malignancy.

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Section: Resultsmentioning

confidence: 99%

A Study of Sonodynamic Therapy of Melanoma C540 Cells in Vitro by Titania/Gold Nanoparticles

2023

J Biomed Phys Eng

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“…Many studies show the potential of ionizing radiations such as X and gamma rays [ 8 ] or even chemotherapy agents [ 9 ] for inducing the bystander effect but a few studies investigated safer cancer treatment modalities such as therapeutic ultrasound (US) in creating this effect [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Bystander Effect of Therapeutic Ultrasound in the Presence of Cisplatin: An in Vitro Study on Human Melanoma Cells

2023

J Biomed Phys Eng

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Background: In the bystander effect, non-irradiated cells receive biological signals from adjacent irradiated cells and undergo a variety of alterations, considered recently in non-ionizing irradiation like ultrasound waves. In this study, the bystander effect of therapeutic ultrasound exposure alone and in combination with cisplatin was determined. Objective: This study aims to determine the bystander effect caused by ultrasound and cisplatin. Material and Methods: This experimental study was conducted on the human melanoma cell line including two groups of target and bystander cells. The target cell group was divided into three sub-groups of ultrasound irradiation alone, cisplatin alone, and ultrasound irradiation in the presence of cisplatin that the culture medium of these three groups of cells was transferred to the bystander cell group using the medium transfer technique. Then, apoptotic bystander cells and the expression of P53 and HO-1 in target and bystander groups were measured. Results: The results of the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and apoptosis assay showed that cell death in target and bystander groups receiving the ultrasound with cisplatin is higher than in the ultrasound without cisplatin. PCR (the polymerase chain reaction) results in the target and bystander groups receiving treatments with increased expression of the P53 gene. Target and bystander groups receiving the ultrasound without cisplatin showed a decrease in HO-1 gene expression, while the ultrasound with cisplatin showed an increase in the HO-1 gene compared to the control group. Conclusion: Combining ultrasound with ultrasound and without it can transfer bystander signals to the cells that are not directly treated.

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The cross-talk between Bax, Bcl2, caspases, and DNA damage in bystander HepG2 cells is regulated by γ-radiation dose and time of conditioned media transfer

2022

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Induction of a bystander effect after therapeutic ultrasound exposure in human melanoma: In-vitro assay

Cited by 5 publications

References 21 publications

A Study of Sonodynamic Therapy of Melanoma C540 Cells in Vitro by Titania/Gold Nanoparticles

A Study of Sonodynamic Therapy of Melanoma C540 Cells in Vitro by Titania/Gold Nanoparticles

Bystander Effect of Therapeutic Ultrasound in the Presence of Cisplatin: An in Vitro Study on Human Melanoma Cells

The cross-talk between Bax, Bcl2, caspases, and DNA damage in bystander HepG2 cells is regulated by γ-radiation dose and time of conditioned media transfer

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