Induction of a cytosolic pyruvate kinase 1 gene during the resistance response to Tobacco mosaic virus in Capsicum annuum

Kim, Ki-Jeong; Park, Chang Jin; Ham, Byung Kook; Choi, Soo Bok; Lee, Boo Ja; Paek, Kyung Hee

doi:10.1007/s00299-005-0082-5

Cited by 24 publications

(11 citation statements)

References 28 publications

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“…Metallothioneins have been shown to be up-regulated due to various viral infections, including Coxsackievirus [25]. PKM2 has presumably its primary role in glycolysis, but pyruvate kinases have also been associated with viral defense reaction in plants [26], and in the induction of human cellular proliferation by viral oncoproteins [27]. RND3 was one of several (including FSCN1 and CKAP4) up-regulated cytoskeleton related genes, suggesting that the cytoskeleton, and possibly membrane transport, are an issue.…”

Section: Resultsmentioning

confidence: 99%

Modulation of gene expression in a human cell line caused by poliovirus, vaccinia virus and interferon

Grinde

Gayorfar

Hoddevik

2007

Virol J

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Background: The project was initiated to describe the response of a human embryonic fibroblast cell line to the replication of two different viruses, and, more specifically, to look for candidate genes involved in viral defense. For this purpose, the cells were synchronously infected with poliovirus in the absence or presence of interferon-alpha, or with vaccinia virus, a virus that is not inhibited by interferon. By comparing the changes in transcriptosome due to these different challenges, it should be possible to suggest genes that might be involved in defense.

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Section: Resultsmentioning

confidence: 99%

Modulation of gene expression in a human cell line caused by poliovirus, vaccinia virus and interferon

Grinde

Gayorfar

Hoddevik

2007

Virol J

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“…Total RNA was isolated from leaf tissues challenged with chemicals or SCC1 as described previously (Kim et al 2006). Total RNA was treated with 1 U of RNase-free DNase (Bioneer, Korea) containing 1 μg of DNase-treated total RNA, oligo primers and Moloney murine leukemia virus reverse transcriptase (Invitrogen, USA).…”

Section: Reverse Transcriptase (Rt)-pcrmentioning

confidence: 99%

2-Aminobenzoic acid of Bacillus sp. BS107 as an ISR determinant against Pectobacterium carotovorum subsp. carotovotrum SCC1 in tobacco

et al. 2010

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Root drench-treatment of tobacco seedlings with Bacillus sp. BS107 (BS107) suppressed disease development caused by the pathogen Pectobacterium carotovorum subsp. carotovotrum SCC1 (SCC1). A determinant of BS107 involved in induced systemic resistance (ISR) against SCC1 was isolated from cell-free culture. ISR bioassay-guided isolation was involved in determining active fractions during chromatography. Mass spectrometry and NMR analyses of the isolated metabolite identified 2-aminobenzoic acid (2-AB) as a main ISR determinant. 2-AB at 2.3 mM suppressed significantly disease development and exhibited no direct contact inhibition of pathogen. Reverse Transcriptase (RT)-PCR analyses of tobacco leaves revealed up-regulation of the induced resistance marker genes such as PR1a, PR1c, PR2 and PR4 by application of 2-AB on the root. Among aminobenzoic acids tested, 2-and 4-aminobenzoic acids showed ISR activity against soft-rot pathogen, but 3-aminobenzoic acid did not. This is the first report that 2-AB exhibits the ISR against SCC1in tobacco. BS107 can play a role in promoting plant defences by secretion of bacterial determinants including 2-AB for elicitation of ISR.

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“…PKp is thought to be critical for supplying pyruvate and ATP to several plastidic biosynthetic pathways ( Knowles et al, 1998 ; Andre et al, 2007 ; Oliver et al, 2008 ), whereas PKc function is more complicated because it plays a major role in a range of biosynthetic pathways as well as respiration ( Knowles et al, 1998 ; Oliver et al, 2008 ). For example, PKc was reported to respond to several defense-related abiotic stresses in Capsicum annuum ( Kim et al, 2006 ) and to regulate pyruvate and alternative oxidase levels in heterotrophic tissues in potato ( Oliver et al, 2008 ). PKc also plays a potential regulatory role in fruit ripening ( Law and Plaxton, 1997 ; Qin et al, 2013 ), with a stronger PKc mRNA expression resulting in a higher fruit sugar content in loquat fruit ( Qin et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of DkPK Genes Associated with Natural Deastringency in C-PCNA Persimmon

Guan

Chen

et al. 2016

Front. Plant Sci.

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Chinese pollination-constant non-astringent (C-PCNA) persimmon (Diospyros kaki Thunb.) is considered to be an important germplasm resource for the breeding of PCNA cultivars, though its molecular mechanisms of astringency removal remain to be elucidated. Previously, we showed that the abundance of pyruvate kinase gene transcripts increased rapidly during astringency removal in C-PCNA persimmon fruit. Here, we report the full-length coding sequences of six novel DkPK genes from C-PCNA persimmon fruit isolated based on a complementary DNA (cDNA) library and transcriptome data. The expression patterns of these six DkPK genes and correlations with the soluble proanthocyanidin (PA) content were analyzed during various fruit development stages in different types of persimmon, with DkPK1 showing an expression pattern during the last stage in C-PCNA persimmon that was positively correlated with a decrease in soluble PAs. Phylogenetic analysis revealed that DkPK1 belongs to cytosolic-1 subgroup, and subcellular localization analysis confirmed that DkPK1 is located in the cytosol. Notably, tissue expression profiling revealed ubiquitous DkPK1 expression in different persimmon organs, with the highest expression in seeds. Furthermore, transient over-expression of DkPK1 in persimmon leaves resulted in a significant decrease in the content of soluble PAs but a significant increase in the transcript levels of pyruvate decarboxylase genes (DkPDC1, -3, -4, -5), which catalyze the conversion of pyruvate to acetaldehyde. Thus, we propose that an acetaldehyde-based coagulation effect reduces the content of soluble PAs. Taken together, our results suggest that DkPK1 might be involved in the natural removal of astringency at the last developmental stage in C-PCNA persimmon. This is the first report to identify several novel full-length DkPK genes as well as their potential roles in the natural loss of astringency in C-PCNA persimmon.

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Induction of a cytosolic pyruvate kinase 1 gene during the resistance response to Tobacco mosaic virus in Capsicum annuum

Cited by 24 publications

References 28 publications

Modulation of gene expression in a human cell line caused by poliovirus, vaccinia virus and interferon

Modulation of gene expression in a human cell line caused by poliovirus, vaccinia virus and interferon

2-Aminobenzoic acid of Bacillus sp. BS107 as an ISR determinant against Pectobacterium carotovorum subsp. carotovotrum SCC1 in tobacco

Isolation and Characterization of DkPK Genes Associated with Natural Deastringency in C-PCNA Persimmon

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