Induction of a heat‐shock‐type response in <i>Saccharomyces cerevisiae</i> following glucose limitation

Bataillé, Nelly; Régnacq, Matthieu; Boucherie, Hélian

doi:10.1002/yea.320070407

Cited by 31 publications

(24 citation statements)

References 44 publications

(33 reference statements)

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“…Several studies revealed major changes in the pattern of gene expression, with the common feature that genes encoding components of the glycolytic pathway and cell growth machinery are repressed, and glucose-repressed genes are de-repressed (Bataillé et al, 1991;Boucherie, 1985;Boy-Marcotte et al, 1987;DeRisi et al, 1997). However, a subset of genes that is characteristic for this phase was already induced when glucose and all other nutrients were still available in the medium, and expression was induced far in advance of other so-called diauxic and stationary phase events (Herman, 2002;Werner-Washburne et al, 1993, 1996.…”

Section: Introductionmentioning

confidence: 99%

HXT5 expression is under control of STRE and HAP elements in the HXT5 promoter

Verwaal

Arako

Kapur

et al. 2004

Yeast

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Hexose transporter (Hxt) proteins transport glucose across the plasma membrane in the yeast Saccharomyces cerevisiae. Recently, we have shown that expression of HXT5 is regulated by the growth rate of the cells. Because gene expression is regulated by binding of specific transcription factors to regulatory elements in the promoters of genes, the presence of putative regulatory elements in the promoter of HXT5 was determined by computer-assisted analysis. This revealed the presence of two putative stress-responsive elements (STREs), one putative post-diauxic shift (PDS) element and two putative Hap2/3/4/5p (HAP) complex binding elements. The involvement of these elements was studied by using mutations in a HXT5 promoter-LacZ fusion construct. Growth during various conditions that result in low growth rates of yeast cells revealed that the STRE most proximal to the translation initiation site seemed to be involved in particular in regulation of HXT5 expression during growth at decreased growth rates. In addition, the HAP elements seemed to be required during growth on non-fermentable carbon sources. The PDS element and, to a lesser extent, the other STRE showed particular involvement in regulation of HXT5 expression during growth on ethanol. Finally, it was shown that the PKA pathway, which is known to be involved in expression of STRE-regulated genes, was also involved in regulation of HXT5 expression. A possible mechanism by which expression of HXT5 could be regulated by the transcriptional regulatory elements in the promoter is discussed.

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Section: Introductionmentioning

confidence: 99%

HXT5 expression is under control of STRE and HAP elements in the HXT5 promoter

Verwaal

Arako

Kapur

et al. 2004

Yeast

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“…Nutritional limitation causes cells to arrest cell growth and enter stationary phase (1)(2)(3). This is a metabolically quiescent state where expression of genes required for survival is induced, whereas expression of cell cycle genes is repressed (4,5). Fission yeast cells enter stationary phase from G 2 upon glucose starvation and from G 1 upon nitrogen starvation (6).…”

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confidence: 99%

The Fission Yeast ES2 Homologue, Bis1, Interacts with the Ish1 Stress-responsive Nuclear Envelope Protein

Taricani¹,

Tejada²,

Young

2002

Journal of Biological Chemistry

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In fission yeast, nutrient starvation induces physiological, biochemical, and morphological changes that enable survival. Collectively these changes are referred to as stationary phase. We have used a green fluorescent protein random insertional mutagenesis system to isolate two novel stress-response proteins required in stationary phase. Ish1 is a nuclear envelope protein that is present throughout the cell cycle and whose expression is increased in response to stresses such as glucose and nitrogen starvation, as well as osmotic stress. Expression of Ish1 is regulated by the Spc1 MAPK pathway through the Atf1 transcription factor. Although overexpression of Ish1 is lethal, cells lacking ish1 exhibit reduced viability in stationary phase. Bis1 is a novel interacting partner of Ish1. Bis1 is the Schizosaccharomyces pombe member of the ES2 nuclear protein family found in Mus musculus, Drosophila melanogaster, Homo sapiens, and Arabidopsis thaliana. Overexpression of Bis1 results in a cell elongation phenotype, whereas bis1 ؊ cells exhibit a reduced viability in stationary phase similar to that seen in ish1 ؊ cells.

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“…Once rapid growth begins, after lag phase, energy is derived primarily from fermentation of glucose. When glucose is exhausted, a transient growth arrest occurs (the diauxic shift), during which time cells adapt to respiratory metabolism (5,30,66). After this arrest, cells grow at a much lower rate for several days prior to entering stationary phase (17,30).…”

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confidence: 99%

“…Stationary phase, defined as the time when there ceases to be a net increase in cell number, is a differentiated state (75,76) during which cells enter a genetically defined offshoot of the cell cycle (41). The synthesis of most postexponential proteins in S. cerevisiae is initiated during the diauxic shift (5,30). In dramatic contrast, synthesis of one protein, Snz1p (previously designated p35) is detected much later than other proteins (30).…”

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A stationary-phase gene in Saccharomyces cerevisiae is a member of a novel, highly conserved gene family

et al. 1996

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The regulation of cellular growth and proliferation in response to environmental cues is critical for development and the maintenance of viability in all organisms. In unicellular organisms, such as the budding yeast Saccharomyces cerevisiae, growth and proliferation are regulated by nutrient availability. We have described changes in the pattern of protein synthesis during the growth of S. cerevisiae cells to stationary phase (E. K. Fuge, E. L. Braun, and M. Werner-Washburne, J. Bacteriol. 176:5802-5813, 1994) and noted a protein, which we designated Snz1p (p35), that shows increased synthesis after entry into stationary phase. We report here the identification of the SNZ1 gene, which encodes this protein. We detected increased SNZ1 mRNA accumulation almost 2 days after glucose exhaustion, significantly later than that of mRNAs encoded by other postexponential genes. SNZ1-related sequences were detected in phylogenetically diverse organisms by sequence comparisons and low-stringency hybridization. Multiple SNZ1-related sequences were detected in some organisms, including S. cerevisiae. Snz1p was found to be among the most evolutionarily conserved proteins currently identified, indicating that we have identified a novel, highly conserved protein involved in growth arrest in S. cerevisiae. The broad phylogenetic distribution, the regulation of the SNZ1 mRNA and protein in S. cerevisiae, and identification of a Snz protein modified during sporulation in the gram-positive bacterium Bacillus subtilis support the hypothesis that Snz proteins are part of an ancient response that occurs during nutrient limitation and growth arrest.The proper regulation of cellular proliferation and growth in response to extracellular cues is critical for development and the maintenance of viability in all organisms (4,45,75,76). The primary regulators of cellular growth and proliferation in multicellular organisms are growth factors and hormones, and the loss of this regulation may lead to neoplasia (4). Similar regulation of growth and proliferation exists in unicellular organisms, such as the budding yeast Saccharomyces cerevisiae, but nutrient availability is typically the most important extracellular cue in unicellular organisms (4,45,75,76). Given the universal distribution of mechanisms for regulating cellular growth and proliferation and the obvious selective advantage of proper growth regulation, it is likely that the last common ancestor of all organisms, termed the cenancestor (22, 26), also had mechanisms to control growth and proliferation in response to nutrient limitation.Aspects of general stress responses, such as the heat shock response, are distributed universally among organisms (18,47). During the heat shock response, synthesis of specific evolutionarily conserved proteins increases (8,15,18,36,47). The increased synthesis of the heat shock proteins occurs in response to increased temperature and other stresses in all three phylogenetic domains (defined in reference 80), suggesting that the heat shock response in ex...

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Induction of a heat‐shock‐type response in Saccharomyces cerevisiae following glucose limitation

Cited by 31 publications

References 44 publications

HXT5 expression is under control of STRE and HAP elements in the HXT5 promoter

HXT5 expression is under control of STRE and HAP elements in the HXT5 promoter

The Fission Yeast ES2 Homologue, Bis1, Interacts with the Ish1 Stress-responsive Nuclear Envelope Protein

A stationary-phase gene in Saccharomyces cerevisiae is a member of a novel, highly conserved gene family

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