Induction of Adventitious Shoots From Internodal Longitudinal Sections of Castanea Sativa Mill.

Mulin, Manorama; Pais, M. S.; Seabra, R. da Costa

doi:10.17660/actahortic.1999.494.38

Cited by 1 publication

(2 citation statements)

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“…In the case of Castanea sativa, we induced adventitious shoots from internodal longitudinal sections isolated from in vitro material established from seedlings and maintained in culture for 3 years (Mulin et al 1999). In the case of Castanea sativa, we induced adventitious shoots from internodal longitudinal sections isolated from in vitro material established from seedlings and maintained in culture for 3 years (Mulin et al 1999).…”

Section: Discussionmentioning

confidence: 99%

“…It is therefore important to define breeding programs so as to improve the quality of the cork oak trees. This method was successfully adapted to Petunia hybrida to obtain floral shoots (Mulin and Tran Thanh Van 1989) and when applied to explants isolated from the nodal region of hypocotyl of 5day-old seedlings of Helianthus annuus and Lupinus mutabilis floral induction was observed in a low number of explants (Mulin and Tran Thanh Van 1994). Furthermore, cork oak reproductive behaviour is very complex and detailed studies on floral biology are needed so as to carry out controlled crossings (Varela and Valdiviesso 1996).…”

Section: Introductionmentioning

confidence: 99%

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In vitro floral induction from thin longitudinal sections and micro-cuttings of juvenile cork oak material

Mulin

Pais

2003

Trees

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In vitro flowers were obtained from thin longitudinal sections excised at the proximity of the apical region or nodal regions of the main stem and axillary shoots of in vitro Quercus suber stock shoots as well as from field-grown seedlings. In vitro floral induction was also achieved from nodal segments of 8to 9-month-old seedlings or from micro-cuttings of embryonic main shoot and its axillary shoots of 2month-old seedlings. The more juvenile the material the shorter the period required to achieve flowering. Under the described experimental conditions we have thus been able to induce the expression of neoteny in a woody, longcycle species such as cork oak.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%