Induction of an Allergic Air-Pouch Inflammation in Rats

Tsurufuji, Susumu; Yoshino, Shigefumi; Ohuchi, Kazuo

doi:10.1159/000233170

Cited by 55 publications

(53 citation statements)

References 14 publications

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“…We have previously shown that humoral immunological responses including anaphylaxis and Arthus type hy persensitivity reactions may be involved in the early phase of this inflammation [40][41][42]. Therefore, dietary enrichment of (n-3)-fatty acids might influence the immunological responses before inflammatory re sponses occurred in the air pouch.…”

Section: Discussionmentioning

confidence: 99%

Effect of a Fish-Oil-Supplemented Diet on Inflammation and Immunological Processes in Rats

Yoshino

Ellis²

1987

Int Arch Allergy Immunol

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The effect of a diet enriched in fish-oil-derived fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DCHA) in inflammation and immunological processes in rats has been investigated. Rats on a normal chow diet were given 500 mg/kg/day EPA and 333 mg/kg/day DCHA by gavage over a period of 50 days. Control groups received water, oleic acid or safflower oil. Acute and chronic phases of inflammation induced by antigenic challenge with bovine serum albumin were examined in the rat air-pouch model. In rats receiving fish-oil-derived fatty acids, there was a reduced production of the arachidonic acid metabolites, prostaglandin E₂ and leukotriene B₄, in the exudate in the chronic phase of inflammation, but not in the acute phase. This was associated with an increased infiltration of leukocytes, especially monocytes and macrophages, in the chronic inflammatory phase. The fish-oil-supplemented diet showed no effect on the volume of inflammatory exudate, the amount of protein in the exudate and connective tissue proliferation. Carrageenin-induced paw edema in the animals was not influenced by the diet. There was no effect of the dietary fish oil on production of antibodies to bovine serum albumin in the rats. However, the diet appeared to suppress a delayed-type skin reaction in the animals. These studies suggest that fish-oil-derived fatty acids may modulate chronic inflammation and a cellular-mediated immunological reaction by reducing the synthesis of arachidonic acid metabolites.

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Section: Discussionmentioning

confidence: 99%

Effect of a Fish-Oil-Supplemented Diet on Inflammation and Immunological Processes in Rats

Yoshino

Ellis²

1987

Int Arch Allergy Immunol

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“…Immunization and induction of allergic air pouch inflamma tion were carried out as described previously [23,24]. Male rats of the Sprague-Dawley strain, specific pathogen-free, weighing 140-150g (Charles River Japan, Inc., Kanagawa, Japan), were used.…”

Section: Induction Of Anaphylactic Reactionmentioning

confidence: 99%

“…In an experimental model of allergic inflammation of air pouch type in rats, which has been devised in our laboratory [23,24], anaphylactic increase of vas cular permeability is induced [25,26] by injecting the antigen solution into the preformed air pouch on the dorsum. The present paper was undertaken to ex amine whether PAF is synthesized in the locus of the inflammatory site in this anaphylactic phase of the allergic inflammation, in order to get further insight into the physiological role of PAF in the anaphylactic reaction.…”

Section: Introductionmentioning

confidence: 99%

Platelet-Activating Factor in the Inflammatory Exudate in the Anaphylactic Phase of Allergic Inflammation in Rats

Watanabe¹,

Ohuchi²,

Sugidachi³

et al. 1987

Int Arch Allergy Immunol

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Using a model of allergic inflammation of air pouch type in rats, the platelet-activating factor (PAF) in the pouch fluid in the anaphylactic phase was analyzed. Anaphylactic reaction was induced by injecting an antigen (azobenzene-arsonate-conjugated acetyl bovine serum albumin) solution into a subcutaneous air pouch preformed on the dorsum of immunized rats. The pouch fluid was collected 30 min after the antigenic challenge, and chloroform extract was subjected to normal phase high-performance liquid chromatography to isolate two fractions, PAF and lyso-PAF. In the pouch fluid, however, there was little activity of PAF as examined by the aggregation of guinea pig platelets. The lyso-PAF fraction obtained was acetylated to PAF chemically with pyridine and acetic anhydride. This acetylated lyso-PAF fraction induced the aggregation of guinea pig platelets, which was inhibited dose-dependently by a PAF antagonist, CV-3988. The amount of lyso-PAF in the pouch fluid of the immunized group in the anaphylactic phase was significantly higher than that of the nonimmunized group. When (³H-)PAF was incubated with the supernatant fraction of the pouch fluid it was metabolized into lyso-PAF time-dependently. The significance of the higher level of lyso-PAF in the pouch fluid in the anaphylactic phase of allergic inflammation is discussed.

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“…During the course of experiments to identify the difference between allergic and non-allergic inflammation employing an air pouch-type inflammation model in rats 1985;Tsurufuji et al, 1982;Hirasawa et al, 1986;Watanabe et al,1987;1990), focused especially on the qualitative difference in the function of infiltrated leucocytes, we found (Watanabe et al, 1994) that the leucocytes ' Author for correspondence. infiltrated into the pouch fluid in the allergic inflammation model are much more active than those in the non-allergic inflammation model with respect to neutrophil chemotactic factor production.…”

Section: Introductionmentioning

confidence: 99%

Possible roles of protein kinases in neutrophil chemotactic factor production by leucocytes in allergic inflammation in rats

Tanabe

Watanabe

Kondoh

et al. 1994

British J Pharmacology

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1 In an air pouch-type allergic inflammation model in rats, leucocytes that had infiltrated into the pouch fluid collected 4 h after the antigen challenge produced proteinaceous chemotactic factors for neutrophils when they were incubated in the medium. 2 To clarify the mechanism of activation of the infiltrated leucocytes in producing these factors, the effects of protein kinase inhibitors on neutrophil chemotactic factor production were examined. 3 When the infiltrated leucocytes were incubated for 4 h in medium containing the non-selective protein kinase inhibitor K-252a (1-100 ng ml-', 2.14-214 nM), the tyrosine kinase inhibitor genistein (1-50 1tgml', 3.7-185 p M), and the more selective protein kinase C inhibitor H-7 (5-100 A gml', 13.7-274 gM); neutrophil chemotactic activity in the conditioned medium was decreased in a concentration-dependent manner, but the adenosine 3':5'-cyclic monophosphate (cAMP)-dependent protein kinase inhibitor H-89 (1-10Ongml1', 2.24-2240nM) showed no effect.4 Isoelectric focusing of the conditioned medium revealed that the leucocytes produced two neutrophil chemotactic factors, leucocyte-derived neutrophil chemotactic factor (LDNCF) 1 and LDNCF-2. Treatment of the leucocytes with K-252a, genistein, and H-7, but not H-89, inhibited production of both LDNCF-1 and LDNCF-2. 5 These results suggest that activation of tyrosine kinase and protein kinase C, but not cAMPdependent protein kinase, is responsible for the production of LDNCF-1 and LDNCF-2. 6 The steroidal anti-inflammatory drug dexamethasone and the protein synthesis inhibitor cycloheximide inhibited neutrophil chemotactic factor production in a concentration-dependent manner. Timecourse experiments showed that the inhibitory effect by dexamethasone was apparent even 30 min after the incubation. 7 Mechanism for inhibiting the production of LDNCF-1 and LDNCF-2 by dexamethasone is also discussed.

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Induction of an Allergic Air-Pouch Inflammation in Rats

Cited by 55 publications

References 14 publications

Effect of a Fish-Oil-Supplemented Diet on Inflammation and Immunological Processes in Rats

Effect of a Fish-Oil-Supplemented Diet on Inflammation and Immunological Processes in Rats

Platelet-Activating Factor in the Inflammatory Exudate in the Anaphylactic Phase of Allergic Inflammation in Rats

Possible roles of protein kinases in neutrophil chemotactic factor production by leucocytes in allergic inflammation in rats

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