Induction of antibodies to plant viral proteins by DNA-based immunization

Production of monoclonal antibodies for immunoaffinity purification and quantitation of Blo t 1 allergen in mite and dust extracts

“…In this study, all mice immunized with Blo t 1 DNA with electroporation produced high titre specific antibodies recognizing both the recombinant and native forms of the allergen. We and others have previously shown that monoclonal and polyclonal antibodies derived from DNA‐immunized animals recognized the recombinant and the native forms of the protein encoded by the immunized DNA [20–25]. This study likewise supports the claim that electroporation enhances gene delivery in vivo resulting in increased expression and elevated immune responses [26, 27].…”

DNA immunization for the production of monoclonal antibodies to Blo t 11, a paramyosin homolog from Blomia tropicalis

“…These data showed that a strong and highly specific response to Blo t 11 could be generated by DNA immunization. It has been previously shown that monoclonal and polyclonal antibodies derived from DNA‐immunized animals recognized the recombinant and the native forms of the protein encoded by the immunized DNA (19, 24–26). The use of electroporation to enhance gene delivery in vivo resulting in increased expression and elevated immune responses has been reported (21, 22).…”

“…Administration of plasmid DNA has been proven to be an effective means to generate humoral immune responses specific to a wide array of proteins (19–26). The type of immune response generated from DNA immunization is influenced by a wide array of factors including the nature of the antigen, the expression vector used, and the route of administration (23, 27).…”

Plant virus incorporated hydrogels as scaffolds for tissue engineering possess low immunogenicity in vivo

“…This rod‐like virus has been used extensively as a model system for studying various aspects of virus–antibody interactions. TMV, when administered with adjuvant, gives very high immunogenicity in vivo at doses as low as micrograms immunization per kilogram in rabbits and mice . TMV has allowed immunologists to study and develop a system that can enhance immune response to pathogenic antigens for vaccine applications .…”

“…TMV, when administered with adjuvant, gives very high immunogenicity in vivo at doses as low as micrograms immunization per kilogram in rabbits and mice. [25][26][27] TMV has allowed immunologists to study and develop a system that can enhance immune response to pathogenic antigens for vaccine applications. 28,29 Gilleland and coworkers constructed the genetically modified chimeric TMV by inserting sequences representing peptide of outer membrane protein F of Pseudomonas aeruginosa between amino acids Ser154 and Gly155 of the TMV coat protein and observed the immunoprotection to the bacteria in a mouse model of chronic pulmonary infection.…”