Induction of Antitumor Immunity by Exosomes Isolated from Cryopreserved Cord Blood Monocyte-Derived Dendritic Cells

Abstract

(1) Background: Dendritic cell (DC) vaccination has shown outstanding achievements in cancer treatment, although it still has some adverse side effects. Vaccination with DC-derived exosomes has been thought to overcome the side effects of the parental DCs. (2) Method: We performed the experiments to check the ability of cryopreserved umbilical cord blood mononuclear cell-derived DCs (cryo CBMDCs) and their exosomes to prime allogeneic T cell proliferation and allogeneic peripheral blood mononuclear cell (alloPBMCs) cytotoxicity against A549 lung cancer cells. (3) Results: We found that both lung tumor cell lysate-pulsed DCs and their exosomes could induce allogeneic T cell proliferation. Moreover, alloPBMCs primed with tumor cell lysate-pulsed DCs and their exosomes have a greater cytotoxic activity against A549 cells compared to unprimed cells and cells primed with unpulsed DCs and their exosomes. (4) Conclusion: Tumor cell lysate-pulsed DCs and their exosomes should be considered to develop into a novel immunotherapeutic strategy—e.g., vaccines—for patients with lung cancer. Our results also suggested that cryo umbilical cord blood mononuclear cells source, which is a readily and available source, is effective for generation of allogeneic DCs and their exosomes will be material for vaccinating against cancer.

Vγ9γδ T Cell Induction by Human Umbilical Cord Blood Monocytes-Derived, Interferon-α-Stimulated Dendritic Cells

“…These results were consistent with the study of Nidea et al (2015), which reported that highly expressing CD56 IFN-DCs induced the proliferation and activation of Vγ9TCR T cells in vitro and in vivo better than conventional IL-4DCs. 22 In a recent published study of our research group, 34 we also generated IL-4DCs from cord blood monocytes and the proliferation of Vγ9γδ T cells stimulated with these IL-4DCs are smaller than that stimulated by IFN-DCs in this current study.…”

Exosomes as Messengers between Mother and Fetus in Pregnancy

“…The unique characteristics of exosomes and their ability to carry cargo to distant destinations in the body makes them ideal candidates to signal between feto-maternal tissues during pregnancy [15]. Studying the contribution of exosomes in pathological pregnancies and related diseases, such as infertility or pregnancy failure can open the way for new exosome-based therapies [118].…”

High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation

“…Monocytes isolated from cryopreserved CB could be potentially transplanted for treating inherited demyelinating conditions of the central nervous system [ 23 ]. Additionally, dendritic cells derived from cryopreserved CB monocytes were observed to exhibit antitumor potential [ 24 ]. Induced pluripotent stem cells were generated from HSCs isolated from 21- to 23.5-year-old cryopreserved CB samples that showed optimal storage recovery of HSCs for future clinical utility [ 22 , 25 ].…”