Induction of Antiviral Response by Electric Pulses

Abstract

Antiviral activity was detected in porcine leukocytes obtained from animals killed in a slaughterhouse. The activity appeared in the supernatant immediately after the addition of Sendai virus, an intended interferon (IFN) inducer. Electroinduction appears to be responsible for the production of antiviral molecules in both porcine and human leukocytes. The macromolecule conferring the antiviral activity is sensitive to heat and pH 2.0 treatment. The preformed or electroinduced antiviral activity originating from porcine leukocytes was not neutralized by either anti-PoIFN-α or anti-PoIFN-γ. The substance involved differs from all known antiviral proteins in that it is not secreted spontaneously into the extracellular space. Its release requires intact and virulent Sendai virus as a trigger signal. Differences in antigenic properties and characteristics indicate that this is a new antiviral substance.

Square-Wave Electric Impulses of 10 ms and 100 V/cm of Field Force, Produced by PGen-1 Impulse Generator Device, Affect the Proliferation Patterns of Different Animal Cells

“…In our experiments, we also found [14] that medium-strength square-wave impulses of direct ionic current (DC) can result in a short-term direct antiviral resistance to virus infections and in a consequence to alter the membrane properties of the target cells [15]. We therefore decided to study the changes in the expression of membrane-bound surface marker molecules that are on the surface of the immune competent cells in the human blood [16][17][18]. Our experiments were aimed to establish whether such exogenous electric stimulation of human leukocytes could be utilized as an immune enhancer and an antiviral protector ex vivo, preferably coupled to the dialysis process.…”

Enhancing Effect of 100.414-kHz Electromagnetic Field Produced by Defender’s Pulse Generator on the ChIFN γ-Like Molecule Inducing Capacity of Lens culinaris Agglutinin and 10% PBS Washouts of Different Holocene Minerals

“…[3][4][5] Pretnar and colleagues 6 reported that one or few strong electric impulses could induce interferon (IFN)-like proteins in human leukocytes that are antigenically different from any contemporary IFN molecule. Kovacs and colleagues 7 found that in porcine leukocytes, the addition of Sendai virus and electric impulses produced porcine interferon a-like molecules. It was found [8][9][10] that 50 Hz EMFs caused phytohemagglutinin (PHA) induction of IFN g and interleukin 6 (IL-6).…”