Induction of apoptosis and cell cycle arrest by Negombata magnifica sponge in hepatocellular carcinoma

Rady, Hanaa M.; Hassan, A.; Salem, Sohair; Mohamed, Tahia K.; Esmaiel, Nora N.; Ez-El-Arab, Mohamed A.; Ibrahim, Mohamed A.; Fouda, Fayez K.

doi:10.1007/s00044-015-1491-9

Cited by 13 publications

(16 citation statements)

References 30 publications

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“…In our previous work, antiproliferative activity of N. magnifica against HepG2 cells was observed through its cytotoxic activity. Primmorph extract arrested the cell cycle in G0-G1 while the mesohyl induced apoptosis [21]. In the present study, mesohyl of N. magnifica induced caspase-independent apoptosis while primmorph extract induced non-apoptotic antiproliferation.…”

Section: Discussionsupporting

confidence: 53%

“…Primmorph extract of H. arabica had no cytotoxic activity. Our previous work revealed that IC 50 values of primmorph extract and mesohyl of N. magnifica were 5 and 3 μg/mL, respectively [21]. For further bioassays, cells were treated with sub-lethal concentrations 1, 5, 5 or 2 μg/mL of primmorph extract or 1, 1, 5 or 5 μL/mL of mesohyl of N. magnifica , H. arabica , C. spinulata, and S. carteri , respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Our previous work [21] revealed that mesohyls of N. magnifica did not exhibit any noticeable effect on cell cycle phases, while primmorph extract decreased the cell population in S-phase and arrested cells in G0–G1. Herein, the cell number in S-phase was decreased and the cells were arrested in G0-G1 after treatment with the primmorph extracts of the three sponge species.…”

Section: Resultsmentioning

confidence: 99%

“…Sponge specimens were dissected under sterile conditions and prepared as previously described by Rady et al. [21]. The mesohyl was aspired carefully and collected with a sterile syringe taking special care not to aspirate any other fluids than the mesohyl.…”

Section: Methodsmentioning

confidence: 99%

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Primmorph extracts and mesohyls of marine sponges inhibit proliferation and migration of hepatocellular carcinoma cells in vitro

Rady

Salem

El-Arab

2019

Journal of Pharmaceutical Analysis

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Cancer recurrence and severe side effects of currently being used chemotherapeutic agents reduce their clinical efficacy. Thus, there is a constant need to develop alternative anticancer drugs. Sustainable supply is an important challenge facing marine-based drug discovery. Primmorph, a 3D cell culture system, could provide a sustainable source to produce metabolites for anticancer drugs from marine sponges. In the present work, the anticancer activity of primmorph extracts and mesohyls of Negombata magnifica , Hemimycle arabica, Crella spinulata , and Stylissa carteri sponges was evaluated. Antiproliferative activity was studied in terms of cytotoxicity, colony formation, cell cycle, and apoptosis. Migration was assessed by migration assay and matrix metalloproteinase activity. The expression of proliferation and migration-related genes was analyzed using real time PCR. Migration and proliferation activities of HepG2 cells were inhibited by treatment with primmorph extracts and mesohyls of N. magnifica , H. arabica , and C. spinulata . The mesohyl of S. carteri did not show any anticancer activity although the primmorph extract led to cell cycle arrest. Among the selected sponge species, the primmorph extract of C. spinulata was the most promising anticancer agent regarding antiproliferative and antimigratory activities. In addition, primmorph extracts have the advantage of working under well-defined and controlled conditions, which allows the easy application as a bioreactor.

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Section: Discussionsupporting

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Primmorph extracts and mesohyls of marine sponges inhibit proliferation and migration of hepatocellular carcinoma cells in vitro

Rady

Salem

El-Arab

2019

Journal of Pharmaceutical Analysis

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“…Previous studies found many natural apoptosis-inducing compounds isolated from marine sponge that can be developed as natural medicine 11 . Fraction of Negombata magnifica sponge is able to induce apoptosis in hepatocellular carcinoma 12 . Sponge extract of Haliclona sp.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis induction on human breast cancer T47D cell line by extracts of Ancorina sp.

Tunjung

Sayekti

2019

F1000Res

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Background: Breast cancer is the second leading cause of death in women. Alternative medicine with high efficacy is needed for breast cancer treatments, for example induction of apoptosis using natural products. It has been found that many natural apoptosis-inducing compounds are isolated from marine sponge. The objective of this study is to analyze the ability of extracts of the sponge Ancorina sp. to induce apoptosis on human breast cancer T47D cell line and find out its mechanism. Methods: T47D cells were treated with crude extracts of methanol, dichloromethane:methanol (1:1) and dichloromethane Ancorina sp. for 24 h, and doxorubicin was used as a positive control. Methods used for this study were MTT assay to examine cell viability and determine IC50 of the three extracts, while the percentage of apoptosis and caspase-3 were investigated by flow cytometry. Results: IC50 values of methanol, dichloromethane:methanol (1:1), and dichloromethane extract were 84.25, 121.45, and 99.85μg/mL respectively. The percentages of apoptotic cells after treatment with methanol, dichloromethane:methanol (1:1), and dichloromethane extracts were 88.68, 27.54 and 53.63% respectively, whereas the percentage of caspase-3 was 77.87, 12.66 and 12.97%, respectively. Conclusions: These results revealed that all extracts of Ancorina sp. have strong or moderate cytotoxicity and have the ability to induce apoptosis on T47D human breast cancer cell line. However, methanol crude extract has high efficacy to induce apoptosis through caspase-3 activation compared to the other extracts. Hence methanol extract warrants further investigation as a natural medicine for human breast cancer.

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Evidences for the antitumor potentiality of Hemimycale arabica and Negombata magnifica mesohyls in hepatocellular carcinoma rat model

2018

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Induction of apoptosis and cell cycle arrest by Negombata magnifica sponge in hepatocellular carcinoma

Cited by 13 publications

References 30 publications

Primmorph extracts and mesohyls of marine sponges inhibit proliferation and migration of hepatocellular carcinoma cells in vitro

Primmorph extracts and mesohyls of marine sponges inhibit proliferation and migration of hepatocellular carcinoma cells in vitro

Apoptosis induction on human breast cancer T47D cell line by extracts of Ancorina sp.

Evidences for the antitumor potentiality of Hemimycale arabica and Negombata magnifica mesohyls in hepatocellular carcinoma rat model

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