Induction of apoptosis by carbazole alkaloids isolated from Murraya koenigii

Ito, Chihiro; Itoigawa, Masataka; Nakao, Keisuke; Murata, Takeshi; Tsuboi, Masaru; Kaneda, Norio; Furukawa, Hiroshi

doi:10.1016/j.phymed.2005.03.010

Cited by 110 publications

(74 citation statements)

References 13 publications

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“…The proteolytic activity of caspase-3 is a critical determinant of whether or not cell commits suicide [41]. It was observed that alkaloids from Murraya has induced the loss of mitochondrial membrane potential and subsequently activated caspase-9/caspase-3 cascade resulting in induction of apoptosis [31]. In the present study, Pb exposure stimulated the specific activity of caspase-3 and it was evident with the significant increase in its levels.…”

Section: Discussionsupporting

confidence: 53%

“…Reported important constituents are carbazole alkaloids, coumarins and flavonoids [25]. Koenidine (KND), 3,11-Dihydro-8,9-dimethoxy-3,3,5-trimethylpyrano [3,2-a]carbazole is an active ingredient said to have antioxidant [26][27][28], antiinflammatory [29], immune-modulatory [30] and apoptotic effects [31][32][33]. Therefore, the present report was focused to elucidate the mitigative properties of KND against Pb-induced toxicity in Human neuroblastoma cells.…”

Section: Introductionmentioning

confidence: 99%

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Mitigative Effects of Koenidine in Reducing the Toxicity Induced by Heavy Metal Lead in Human Neuroblastoma Cells

Challa¹

2016

J Chem Biol Ther

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Lead is a heavy metal been widely used in the manufacturing of glass, pigments, makeup, pipes, wine, cooking, electronic components and batteries. It is a multimedia environmental pollutant, and its exposure via different routes is found to affect metabolic functions. Primarily Lead (Pb) plays an important role in inducing oxidative stress one of the important pathologies and can affect the functioning of antioxidant defense system. The cell growth and proliferation is severely affected with the exposure of lead in designated quantities. There is no specific therapy available to treat the toxicity being generated by Pb and the efforts are on to find suitable natural biologically active components that can effectively combat the toxicity. Koenidine (KND) one of the active ingredients from the plant Murraya koenigii was considered to test its efficacy in mitigating the toxicity elicited by Pb. Human SH-SY5Y neuroblastoma cells were exposed to different concentrations (0.01-10 μM) of Pb for 48 h and the cell viability was determined. The IC50 was observed at 5 μM and this particular concentration used for further investigations. The cells were also pre-treated with KND with a range of concentration from 0-100 μM for 48 h, no change in the cell viability was observed. KND was treated (50 μM) to the cells to verify its protective effects against the pre-treatment of Pb at a concentration of 5 μM 48 h, resulted in the significant increase in the cell viability at least by 29.4% from 12.1% when compared with Pb alone treated group. Intracellular glutathione, caspase-3 and prostaglandin E2 levels were quantified to find the protective effects of koenidine. Some significant results (but less than 18%) were observed in decreasing the oxidative stress, combating apoptosis and lessening the inflammation. This clearly indicates that koenidine can be effectively used to mitigate the toxicity provoked by Pb indicating the nature of koenidine as an effective antioxidant, anti-apoptotic, and anti-inflammatory agent.

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Section: Discussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Mitigative Effects of Koenidine in Reducing the Toxicity Induced by Heavy Metal Lead in Human Neuroblastoma Cells

Challa¹

2016

J Chem Biol Ther

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“…Leaf extract of Murraya koenigii have also been reported to posses antifungal, antineoplastic, nitric oxide scavenging and antioxidant activities (Das et al, 1965;Khan et al, 1996a;Chakrabarty et al, 1997;Khan et al, 1997;Ramsewak, et al, 1999;Tachibana et al, 2001;Baliga et al, 2003). Anti-oxidant, Antitumor, Anti-microbial and Anti-diabetic actives have been reported for some carbazole alkaloids, which were identified as the major compounds in Murraya koenigii leaves (Tachibana et al, 2001;Adebajo et al, 2004Adebajo et al, , 2006Ito et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Nootropic Potential of Murraya koenigii leaves in Rats

Mani¹,

Parle²,

Sengottuvelu³

et al. 2008

Oriental Pharmacy and Experimental Medicine

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SUMMARYMurraya koenigii leaves commonly known as 'curry patta' are routinely added to Indian gravy and vegetarian dishes by south Indian as a favourate condiment. The present study was undertaken to investigate the effects of Murraya koenigii leaves (MKL) on memory in rats. Elevated plus-maze and Hebb-Williams maze served as the exteroceptive behavioral models for testing memory. Diazepam-, scopolamine-and ageing-induced amnesia served as the interoceptive behavioral models. MKL fed orally to various groups of young and aged rats with diet containing 2, 4 and 8% w/w of MKL for 30 days consecutively were investigated. The MKL diets produced a significant dose-dependent improvement in memory scores of young and aged rats and significantly reduced the amnesia induced by scopolamine (0.4 mg/kg, i.p.) and diazepam (1 mg/kg, i.p.). Therefore, it would be worthwhile to specifically investigate the therapeutic potential of MKL in the management of dementia patients.

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“…Activation of caspase-3 may be a critical therapeutic target for prostate cancer treatment. Alkaloids activate cellular caspase-3-like activity and up-regulate expression of caspase-3 in various cancer cell lines (Fil'chenkov et al, 2006;Ganguly and Khar, 2002;Deng et al, 2006;Ito et al, 2006;Griffin et al, 2007;Griffin et al, 2011). To the best of our knowledge, the direct effect of alkaloids on human recombinant caspase-3 has not been reported, though the effect of alkaloids on prostate cancer cell lines (e.g.…”

Section: Introductionmentioning

confidence: 99%

Effect of erythrinaline alkaloids from Erythrina lysistemon on human recombinant caspase-3

Armwood¹,

Juma²,

Ombito³

et al. 2018

Afr. J. Pharm. Pharmacol.

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Prostate cancer is a leading killer disease among men all over the world. Inducing apoptosis (programmed cell death) is a strategic chemotherapeutic approach. Caspase-3 is a key effector of apoptosis, and its activation promotes apoptosis. It was hypothesized that erythrinaline alkaloids activate caspase-3. The alkaloids were isolated from the flowers and pods of Erythrina lysistemon. Their effect on human recombinant caspase-3 was studied. This study reports that three erythrinaline alkaloids (+)-11α-hydroxyerysotrine N-oxide (1), (+)-11β-hydroxyerysotrine N-oxide (2) and (+)-11β-methoxyerysotrine N-oxide (3) activated human recombinant caspase-3 in a dose-dependent manner. Compound 1 and 2 increased the activity by five-fold while compound 3 increased it by ten-fold. Erythrinaline alkaloids exhibit remarkable ability to activate caspase-3 and may be lead compounds as potential therapeutics for the treatment of cancer as inducers of apoptosis in cancer cells.

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Induction of apoptosis by carbazole alkaloids isolated from Murraya koenigii

Cited by 110 publications

References 13 publications

Mitigative Effects of Koenidine in Reducing the Toxicity Induced by Heavy Metal Lead in Human Neuroblastoma Cells

Mitigative Effects of Koenidine in Reducing the Toxicity Induced by Heavy Metal Lead in Human Neuroblastoma Cells

Nootropic Potential of Murraya koenigii leaves in Rats

Effect of erythrinaline alkaloids from Erythrina lysistemon on human recombinant caspase-3

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