Induction of apoptosis in cancer cell lines by the Red Sea brine pool bacterial extracts

Sagar, Sunil; Esau, Luke; Holtermann, Karie; Hikmawan, Tyas I.; Zhang, Guishan; Stingl, Ulrich; Bajic, Vladimir B.; Kaur, Mandeep

doi:10.1186/1472-6882-13-344

Cited by 38 publications

(35 citation statements)

References 77 publications

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“…Red Sea microbial diversity (bacteria/fungi) are interesting objects for further studies to discover novel metabolites and new chemotherapeutic agents. Recently, Sagar et al have shown significant cytotoxic activities of some halophilic bacterial species that were isolated from Red sea (Jeddah), and subsequently demonstrated the possible mechanisms of anticancer effect of bacterial extracts on different cancer cell lines [28,29]. While in another study, they have observed significant apoptotic and cytotoxic activities in cancer cell lines treated with marine bacterial extracts that were isolated from brine-seawater interface of the Red Sea.…”

Section: Red Sea Microbial Diversity For Anticancer and Antimicrobialmentioning

confidence: 93%

Red Sea Microbial Diversity for Antimicrobial and Anticancer Agents

M¹

2016

J Mol Biomark Diagn

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Marine natural products are valuable sources that could produce potential chemotherapeutic agents, and marinederived organisms are widely recognized as important sources of these natural products with interesting biological activities. Furthermore, Red sea was recognized as a rich source of microbial diversity with unique metabolites that can be of pharmaceutical and medicinal importance. The discovery of novel active metabolites provides an intellectual challenge to examine new products for medicinal purposes. Although many bacterial strains were isolated from Red sea with potent bioactivities; however, the microbial diversity of marine environment such Red sea remained largely un-explored. Lately, it has been possible to easily collect marine samples, isolate, and further identify diverse marine organisms (e.g. bacteria, fungi, and algae and marine invertebrate) by modern molecular techniques. Based on a wide range of screening and extraction methods, a vast number of metabolites have been extracted from marine sources with potential biological activities such as antibacterial, anticancer, anti-inflammatory agents and many others. In this article, we have reviewed the previous studies that have been conducted to explore marine-derived bioactives of Red sea. In addition, we have illustrated the following aspects: (i) Red sea microbial diversity for antibacterial and anticancer agents; (ii) the conventional and molecular approach used for screening and characterization marine microorganisms; (iii) characterization of natural products of microbial origin, and (iv) drug development from novel marine-derived metabolites and clinical trials. Moreover, modern molecular technologies, meta-genomic, proteomic and bio-chemo-informatics approaches are adding more potency to the targeted search of bioactive compounds derived from marine microbes. The novel marine derived drug discovery is aimed to improve our understanding of natural resources, to improve public health awareness about the environmental microbial diversity and their importance for nutraceuticals, pharmaceutical, agrochemicals and food processing. Recently, the research area of microbial diversity of the Red sea and their metabolites has opened a new access for significant sources of more effective drug entities with low coast.extreme conditions such as high temperature, pressure, and salinity. The experimental results suggested that these bacterial and archaeal communities are rich sources of active enzymes and metabolites, which can be applied for pharmaceutical and biotechnology-based applications [9].In previous studies, a number of bio-active compounds have been reported from marine sources with different degrees of action and potential, such as antibacterial, anticancer, antitumor, antiproliferative, anti-microtubule, cytotoxic, photoprotective, and antifouling properties [10][11][12]. Marine-derived metabolites, like peptides (ribosomal and non-ribosomal), alkaloids, polyketides and terpenes, have demonstrated antimicrobial and antiviral activ...

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Section: Red Sea Microbial Diversity For Anticancer and Antimicrobialmentioning

confidence: 93%

Red Sea Microbial Diversity for Antimicrobial and Anticancer Agents

M¹

2016

J Mol Biomark Diagn

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“…The extracts from Halomonas meridiana strain P3-37B and Idiomarina loihiensis strain P3-37C were unable to induce any change in MMP in HeLa cancer cells and thus suggested a mitochondria-independent apoptosis induction. However, further detection of a PARP-1 cleavage product and the observed changes in Caspase-8 and Caspase-9 suggested the involvement of caspase-mediated apoptotic pathways [109]. An ethyl acetate extract from Streptomyces sp.…”

Section: Bacteriamentioning

confidence: 98%

Halophiles and Their Biomolecules: Recent Advances and Future Applications in Biomedicine

2019

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The organisms thriving under extreme conditions better than any other organism living on Earth, fascinate by their hostile growing parameters, physiological features, and their production of valuable bioactive metabolites. This is the case of microorganisms (bacteria, archaea, and fungi) that grow optimally at high salinities and are able to produce biomolecules of pharmaceutical interest for therapeutic applications. As along as the microbiota is being approached by massive sequencing, novel insights are revealing the environmental conditions on which the compounds are produced in the microbial community without more stress than sharing the same substratum with their peers, the salt. In this review are reported the molecules described and produced by halophilic microorganisms with a spectrum of action in vitro: antimicrobial and anticancer. The action mechanisms of these molecules, the urgent need to introduce alternative lead compounds and the current aspects on the exploitation and its limitations are discussed.

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“…The following day OD (optical density) at 595 nm was measured with a microtiter plate reader (BMG Labtech PHERAstar FS, Germany). [22]. The cells were also incubated with 1 µg/ml Hoechst (Pierce) dye at room temperature for 15 min and visualized using a EVOS® FLoid® Cell Imaging Station (Life Technologies, UK).…”

Section: Mtt Assaymentioning

confidence: 99%

“…Protein lysate (20 µg) underwent electrophoresis on 10% SDS page gels, was transferred to nitrocellulose membranes and probed with antibodies specific to PARP-1 (Trevigen), Caspase-7 (Sigma) and p53 (Santa Cruz Biotechnolgy). For a loading control β-Tubulin (Santa Cruz Biotechnolgy) was used [22].…”

Section: Western Blottingmentioning

confidence: 99%