Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder

Suo, Huayi; Song, Jia‐Le; Zhou, Yalin; Liu, Zhenhu; Yi, Ruokun; Zhu, Kai; Xie, Jing; Zhao, Xinquan

doi:10.3892/ol.2014.2756

Cited by 11 publications

(8 citation statements)

References 28 publications

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“…However, polysaccharides from red algae (ASPE) had a reported effect of low concentrations (50 µg/mL) causing an apoptosis of 50% of cells [ 77 ]. Polysaccharides from Larimichthys crocea swim bladder induced 37.2% apoptosis of HCT-116 cancer cell line in a concentration of 400 µg/mL [ 78 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

et al. 2022

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There is a global need to discover effective anti-cancerous compounds from natural sources. Cultivated wheat cells can be a valuable source of non-toxic or low toxic plant-derived polysaccharides. In this study, we evaluated the anti-cancer ability of seven fractions of wheat cell culture polysaccharides (WCCPSs) in the HCT-116 colon cancer cell line. Almost all (6/7) fractions had an inhibitory effect on the proliferation of colon cancer cells, and two fractions (A-b and A-f) had considerable therapeutic indexes. The WCCPS fractions induced cell cycle arrest in the G1 phase and induced different rates of apoptosis (≤48%). Transmission and scanning electron microscopy revealed that WCCPS fractions caused apoptotic changes in the nucleus and cytoplasm, including damage to mitochondria and external morphological signs of apoptosis. In addition, the WCCPSs induced an increase in the levels of Bax, cytochrome c, and caspases 8 and 3, indicating that cell death progressed through intrinsic and extrinsic pathways of apoptosis. Furthermore, some fractions caused a significant decrease of c-Myc, b-catenin, NFkB2, and HCAM (CD 44) levels, indicating enhanced cell differentiation. Thus, for the first time, our results provide a proof of concept of the anti-cancer capacity of WCCPS fractions in colorectal cancer.

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Section: Discussionmentioning

confidence: 99%

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

et al. 2022

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“…Increasing evidence has identi ed natural product might control cancer via the direct or indirect modulation of apoptosis-related genes 30 . RT-PCR was used to investigate the expression of caspase-3, Bax, Bcl-2, and Bad at different time points.…”

Section: Effects Of Iop60b On Bcl-2 Bax and Caspase-3 Expressions Imentioning

confidence: 99%

The apoptotic effect of polysaccharides with specific molecular weight extracted from Inonotus obliquus on HT-29 colon cancer cells

Han

Zhao

Wang

et al. 2020

Preprint

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Background: Recently, natural products, particularly Inonotus obliquus (I. obliquus), have attracted lots of attention due to their effective anticancer effects with relatively low toxicity. Results: I. obliquus polysaccharides (IOP) with different molecular weights were obtained by filtering the aqueous extract through fractional membrane. IOP60b (10 kDa ≤ molecular weight ≤ 30 kDa) with the highest yield and inhibition ratio of HT-29 cancer cells was chosen to evaluate the apoptotic effect on HT-29 cancer cells. After treated with different concentrations of IOP60b, morphological changes including cell shrinkage and nuclear condensation and DNA fragmentation of HT-29 cancer cells were observed. In addition, the proportions of cells in early apoptosis and late apoptosis were significantly (p<0.05) increased in a dose-dependent manner. To further explore the underlying mechanism, RT-PCR and Western blotting were employed. Results indicated that both Bcl-2 family and Caspase family were involved in the regulation process of apoptosis and IOP60b induced cellular apoptosis via upregulation of Bax/Bcl-2 ratio and activation of Caspase-3.Conclusion: These data suggested that IOP60b could be a potential candidate for the clinical prevention and treatment of colorectal cancer.

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“…Increasing evidences have identi ed natural product might control cancer via the direct or indirect modulation of apoptosis-related genes 30 . RT-PCR was used to investigate the expression of caspase-3, Bax, Bcl-2, and Bad at different time points.…”

Section: Effects Of Iop60b On Bcl-2 Bax and Caspase-3 Expressions Imentioning

confidence: 99%

The Apoptotic Effect of Polysaccharides with Specific Molecular Weight Extracted from Inonotus Obliquus on HT-29 Colon Cancer Cells

Han

Zhao

Wang

et al. 2020

Preprint

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Background: Recently, much attention has been paid to natural products owing to their effective anticancer effects with relatively low toxicity, especially Inonotus obliquus (I. obliquus).Results: Polysaccharides with different molecular weights were filtered from water extract of I. obliquus using grading membrane filtration method. IOP60b (10 kDa ≤ molecular weight ≤ 30 kDa) was found to have the highest yield and the highest inhibition ratio of HT-29 cancer cells, therefore it was chosen to evaluate the apoptotic effect of HT-29 cancer cells. After treated with different concentrations of IOP60b, morphological changes including cell shrinkage and nuclear condensation and DNA fragmentation in HT-29 cancer cells were observed, and cells in early apoptosis and late apoptosis significantly (p<0.05) increased in a dose-dependent manner by arresting cell cycle at G0/G1 phase. To further explore the underlying mechanism, RT-PCR and Western blotting were applied. Results indicated that both Bcl-2 family and Caspase family were involved in the process of apoptosis regulation and IOP60b induced cellular apoptosis via upregulation of Bax/Bcl-2 ratio and activation of Caspase-3.Conclusion: These data suggested that IOP60b might be the potential candidate for the clinical prevention and treatment of colorectal cancer.

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Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder

Cited by 11 publications

References 28 publications

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

The apoptotic effect of polysaccharides with specific molecular weight extracted from Inonotus obliquus on HT-29 colon cancer cells

The Apoptotic Effect of Polysaccharides with Specific Molecular Weight Extracted from Inonotus Obliquus on HT-29 Colon Cancer Cells

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