Induction of apoptosis in HepG2 cells by solanine and Bcl-2 protein

Ji, Yu; Gao, Shuangquan; Ji, Chen; Zou, Xiang

doi:10.1016/j.jep.2007.09.023

Cited by 121 publications

(93 citation statements)

References 26 publications

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“…In addition, solanine effectively reduced the sialic acid level and membrane closing ability of the tumor cells. A previous study demonstrated that the mechanism by which solanine induces human hepatocellular carcinoma HepG2 cells to undergo apoptosis appeared to be mediated by the inhibition of B-cell lymphoma-2 expression (18). Furthermore, solanine has been demonstrated to increase the permeability of the mitochondrial membrane, which leads to the release of apoptosis-associated proteins and inner mitochondrial calcium ions, resulting in tumor cell apoptosis through the mitochondrial pathway (19,20).…”

Section: Introductionmentioning

confidence: 99%

Solanine-induced reactive oxygen species inhibit the growth of human hepatocellular carcinoma HepG2 cells

Meng

Zhang

et al. 2016

Oncology Letters

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Abstract. The aim of the present study was to investigate the effect of solanine on promoting human hepatocellular carcinoma HepG2 cells to produce reactive oxygen species (ROS), and the molecular mechanisms leading to tumor cell apoptosis. Solanine was administered to HepG2 cells in vitro. A selection of probes targeting various cellular localizations of ROS were used to detect ROS expression using flow cytometry. The expression levels of apoptosis-associated proteins, including apoptosis signal-regulating kinase 1 (ASK1) and thioredoxin binding protein 2 (TBP-2), and proliferation-associated proteins, including histone deacetylase 1 (HDAC1), were detected using western blotting. The percentage of cells undergoing apoptosis was measured using an Annexin V-fluorescein isothiocyanate/propidium iodide assay, and cell morphology was examined using Wright's stain followed by inverted microscopy analysis. ROS detection probes 2',7'-dichlorofluorescin diacetate and dihydrorhodamine 123 identified that abundant ROS, including hydroxyl radical (OH -) and hydrogen peroxide (H 2 O 2 ), were produced in the cytoplasm and mitochondria of the solanine-treated HepG2 cells compared with the control cells (P<0.05). Superoxide anion specific probes dihydroethidium and MitoSOX™ demonstrated that there were no significant alterations in the HepG2 cells following solanine treatment compared with the control cells (P>0.05). Western blotting results revealed that solanine upregulated the expression levels of ASK1 and TBP-2 and enhanced their kinase activities, whereas solanine decreased the expression level of the proliferation-associated protein, HDAC1. The cell apoptotic rate was significantly increased (P<0.0001) in the solanine-treated HepG2 cells compared with the control cells. (P<0.05). Overall, the study indicated that solanine induces HepG2 cells to produce ROS, mainly OH -and H 2 O 2 , in a mitochondria-dependent and -independent manner. In addition, solanine stimulates the expression of ASK1 and TBP-2, and their kinase activities, but inhibits the expression of proliferation-associated proteins, such as HDAC1, thus contributing to HepG2 cell apoptosis.

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Section: Introductionmentioning

confidence: 99%

Solanine-induced reactive oxygen species inhibit the growth of human hepatocellular carcinoma HepG2 cells

Meng

Zhang

et al. 2016

Oncology Letters

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“…Chinese herbal antitumor agents, including assolanine (6), an extract from Rhodiola rosea rhizomes (7), Gleditsioside E from Gleditsia sinensis (8) and saponins from Gleditsia sinensis (9), exert significant effects against a variety of tumors and have been confirmed to be effective antitumor agents. Saffron is a derived plant product from the dried stigma of the Crocus sativus flower (family, Iridaceae), which has been used for several hundred years in the treatment of cardiovascular diseases, inflammatory diseases and several other tumors (10,11).…”

Section: Introductionmentioning

confidence: 99%

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Wang

et al. 2013

Molecular Medicine Reports

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Abstract. Gastric cancer (GC) is one of the most common types of gastrointestinal tumors worldwide, and the side effects of chemotherapeutic drugs and the resistance to chemotherapy remain problematic in its clinical treatment. Therefore, safe and effective novel agents are urgently required. The purpose of the present study was to investigate the crocetin-sensitive treatment of GC and its possible mechanisms. BGC-823 human GC cells were treated with crocetin. The effects of crocetin on the viability of the cells were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Hoechst 33258 dyeing and Rh123 staining were used to detect cell apoptosis. The BGC-823 cells were subjected to western blotting analysis for detection of cytochrome c and cleaved caspase-3 protein expression. Crocetin inhibited the proliferation of the GC cell line in a dose-and time-dependent manner. Apoptotic BGC-823 cells induced by crocetin were stained by Hoechst 33258 and observed under a light microscope for cell membrane staining of dense nuclei, nuclear pyknosis, fragmentation, chromatin condensation and highlighted nuclear membrane staining. This revealed a decline in the mitochondrial membrane potential of the BGC-823 cells. Crocetin also induced caspase-3 activation and cytochrome c translocation into the cytosol from the mitochondria. The results of this study indicate that crocetin induces the apoptosis of BGC-823 cells, and may be used as an effective agent in the treatment of GC.

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“…Steroidal alkaloids, such as solamargine induces apoptosis (Shiu et al, 2009) and also solanine induced apoptosis in HepG(2) cells (Ji et al, 2008). Solamargine and solasonine, exhibited an inhibitory effect on serum glucose levels in oral sucrose-loaded rats, and this effect could be relevant for the prevention and treatment of diabetes (Yoshikawa et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Plants from Solanaceae family with possible anxiolytic effect reported on 19th century's Brazilian medical journal

Giorgetti¹,

Negri²

2011

Rev. bras. farmacogn.

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Abstract:In the beginning of the 19 th century, the first Brazilian scientific knowledge journal on medicinal plants the Gazetas Médicas was launch reporting Brazilian medicinal plants belonging to several botanical families. The aim of this study was research the Solanaceae species that were described as anxiolytics in the 19 th century's Brazilian Medical Gazettes and to make a revision about these species in literature. A taxonomic update, together a careful research about ethnopharmacological, pharmacological and phytochemical, patent process and reports of phytomedicines, was carried out for these Solanaceae species. In this research were found thirteen plants, but the taxonomic update reduced this number to six species. Among them, Physalis angulata L. and Solanum nigrum L. were studied and showed depressor activity on central nervous system (CNS) as described in Gazetas Médicas. This research showed that Solanaceae species reported in this study has potential as anxiolytic drugs and should be investigated more deeply.

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Induction of apoptosis in HepG2 cells by solanine and Bcl-2 protein

Cited by 121 publications

References 26 publications

Solanine-induced reactive oxygen species inhibit the growth of human hepatocellular carcinoma HepG2 cells

Solanine-induced reactive oxygen species inhibit the growth of human hepatocellular carcinoma HepG2 cells

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Plants from Solanaceae family with possible anxiolytic effect reported on 19th century's Brazilian medical journal

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