Induction of apoptosis in human pancreatic MiaPaCa-2 cells through the loss of mitochondrial membrane potential (ΔΨm) by Gentiana kurroo root extract and LC-ESI-MS analysis of its principal constituents

Wani, Bilal Ahmad; Duraisamy, Revathi; Rather, Manzoor A.; Arumugam, N.; Qazi, Asif Khurshid; Majeed, Rabiya; Hamid, Abid; Ganie, Showkat Ahmad; Ganie, Showkat Ahmad; Anand, Rajneesh; Gupta, Ajai Prakash

doi:10.1016/j.phymed.2013.01.011

Cited by 46 publications

(27 citation statements)

References 27 publications

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“…The results of LC/MS analysis showed the presence of several compounds which may be potentially responsible for the protective effects of the extract. These results are consistent with previous findings on other Gentiana species [19].…”

Section: Discussionsupporting

confidence: 94%

<i>Gentiana macrophylla</i> Pall (Gentianaceae) extract exerts protective effects against osteoporosis in mice

Gong¹,

Su²,

Xiang³

et al. 2018

Trop. J. Pharm Res

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Purpose: To evaluate the anti-osteoporotic effect of G. macrophylla extract in a mice model. Methods: Antioxidant activity was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) for radical scavenging, nitro-blue tetrazolium (NBT) reducing, and ferric reducing antioxidant power (FRAP) assays using standard biochemical procedures. The expression of some bone-related genes was measured by quantitative real-time polymerase chain reaction (qTR-PCR). Phytochemical analysis was carried out by liquid chromatography-mass spectrometry (LC/MS

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Section: Discussionsupporting

confidence: 94%

<i>Gentiana macrophylla</i> Pall (Gentianaceae) extract exerts protective effects against osteoporosis in mice

Gong¹,

Su²,

Xiang³

et al. 2018

Trop. J. Pharm Res

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“…It was reported that the increased ratio of Bax/Bcl-2 leads to MMP disruption and cytochrome c release, which in turn activates the caspase cascade32 ) . Moreover, it was shown that disruption of MMP is one of the earliest intracellular events that occur during the initial phase of apoptosis via the mitochondria-mediated death pathway33 ) . Lots of evidences indicated that the loss of MMP was associated with the apoptosis induced by some toxicants34, 35 ) .…”

Section: Discussionmentioning

confidence: 99%

2,5-hexanedione induced apoptosis in mesenchymal stem cells from rat bone marrow via mitochondria-dependent caspase-3 pathway

et al. 2015

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2,5-hexanedione (HD) induces apoptosis of nerve cells. However,the mechanism of HD-induced apoptosis remains unknown. Mesenchymal stem cells (MSCs) are multipotential stem cells with the ability to differentiate into various cell types. This study is designed to investigate the apoptosis induced by HD in rat bone marrow MSCs (BMSCs) and the related underlying mechanisms. The fifth generation of MSCs was treated with 0, 10, 20 and 40 mM HD respectively. The viability of BMSCs was observed by MTT. Apoptosis were estimated by Hoechst 33342 staining and TUNEL assay. The disruption of mitochondrial transmembrane potential (MMP) was examined by JC-1 staining. Moreover, the expression of Bax and Bcl-2, cytochrome c release, and caspase-3 activity were determined by real-time RT-PCR, Western blot and Spectrophotometry. Our results showed that HD induced apoptosis in MSCs in a dose dependent manner. Moreover, HD downregulated the Bcl-2 expression,upregulated the Bax expression and the Bax/Bcl-2 ratio, promoted the disruption of MMP, induced the release of cytochrome c from mitochondria to cytosol, and increased the activity of caspase-3 in MSCs. These results indicate that HD induces apoptosis in MSCs and the activated mitochondria-dependent caspase-3 pathway may be involved in the HD-induced apoptosis.

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“…punctata (Sakamoto et al ., ; Li et al ., ; Singh et al ., ; Menkovic et al ., ). Swertianolin has been reported to possess hepatoprotective, anticholinesterase, antituberculosis, antioxidant, antiarrhythmic and antidiabetic effects (Brahmachari et al ., ; Li et al ., ; Urbain et al ., , ; Mukherjee et al ., ; Wani et al ., ; Wan et al ., ). Despite its various biological activities, the pharmacokinetic properties of swertianolin have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Determination of swertianolin in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study

Tian

Ouyang

et al. 2014

Biomedical Chromatography

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A sensitive and rapid LC-MS/MS method has been developed and validated for quantifying swertianolin in rat plasma using rutin as an internal standard (IS). Following liquid-liquid extraction with ethyl acetate, chromatographic separation for swertianolin was achieved on a C18 column with a gradient elution using 0.1% formic acid as mobile phase A and acetonitrile as mobile phase B at a flow rate of 0.3 mL/min. The detection was performed on a tandem mass spectrometer using multiple reaction monitoring via an electrospray ionization source and operating in the negative ionization mode. The optimized mass transition ion pairs (m/z) for quantitation were 435.1/272.0 for swertianolin and 609.2/300.1 for IS. The lower limit of quantitation was 0.5 ng/mL within a linear range of 0.5-500 ng/mL. Intra-day and inter-day precision was less than 6.8%. The accuracy was in the range of -13.9 to 12.0%. The mean recovery of swertianolin was >66.7%. The proposed method was successfully applied in evaluating the pharmacokinetics of swertianolin after an oral dose of 50 mg/kg Swertia mussotii extract in rats.

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Induction of apoptosis in human pancreatic MiaPaCa-2 cells through the loss of mitochondrial membrane potential (ΔΨm) by Gentiana kurroo root extract and LC-ESI-MS analysis of its principal constituents

Cited by 46 publications

References 27 publications

<i>Gentiana macrophylla</i> Pall (Gentianaceae) extract exerts protective effects against osteoporosis in mice

<i>Gentiana macrophylla</i> Pall (Gentianaceae) extract exerts protective effects against osteoporosis in mice

2,5-hexanedione induced apoptosis in mesenchymal stem cells from rat bone marrow via mitochondria-dependent caspase-3 pathway

Determination of swertianolin in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study

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