Induction of apoptosis in human leukemia cells by black tea and its polyphenol theaflavin

Kundu, Trina; Dey, Subhabrata; Roy, Madhumita; Siddiqi, Maqsood; Bhattacharya, R.K.

doi:10.1016/j.canlet.2004.12.035

Cited by 79 publications

(58 citation statements)

References 33 publications

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“…It has been well documented that EGCG induces apoptosis in a variety of cancer cells including HL-60 cells (2,9,14,18). In the present study, we confirmed these previous findings.…”

Section: Discussionsupporting

confidence: 83%

“…In 1996, Hibasami et al (7) demonstrated the first evidence that catechins such as ( − )-epigallocatechin-3-O-gallate (EGCG) induce programmed cell death or apoptosis. Since then, many studies have shown that catechins induce apoptosis in a variety of cancer cells including human leukemia HL-60 cells (2,9,14,28). It has been shown that EGCG's effects on cell growth differ between tumor cells and normal counterparts.…”

mentioning

confidence: 99%

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Differentiation-associated alteration in sensitivity to apoptosis induced by (-)-epigallocatechin-3-O-gallate in HL-60 cells

et al. 2009

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“…It has been well documented that EGCG induces apoptosis in a variety of cancer cells including HL-60 cells (2,9,14,18). In the present study, we confirmed these previous findings.…”

Section: Discussionsupporting

confidence: 83%

mentioning

confidence: 99%

Differentiation-associated alteration in sensitivity to apoptosis induced by (-)-epigallocatechin-3-O-gallate in HL-60 cells

et al. 2009

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“…Many researchers have demonstrated that the anticancer activities of certain chemotherapeutic agents are involved in the induction of apoptosis, which has become a principal mechanism for effective anticancer therapy (Kundu et al, 2005;Liu et al, 2012). In this study, we showed SBC could decrease the survival rate of human gastric cancer SGC-7901 cells in a concentrationand time-dependent manner, and the inhibitory activities of SBC were stronger than those of SAC ( Figure 1A and Figure 1B).…”

Section: Discussionmentioning

confidence: 51%

S-benzyl-cysteine-mediated Cell Cycle Arrest and Apoptosis Involving Activation of Mitochondrial-dependent Caspase Cascade through the p53 Pathway in Human Gastric Cancer SGC-7901 Cells

Sun

Meng²,

Shen³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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S-benzyl-cysteine (SBC) is a structural analog of S-allylcysteine (SAC), which is one of the major watersoluble compounds in aged garlic extract. In this study, anticancer activities and the underlying mechanisms of SBC action were investigated and compared these with those of SAC using human gastric cancer SGC-7901 cells. SBC significantly suppressed the survival rate of SGC-7901 cells in a concentration-and time-dependent manner, and the inhibitory activities of SBC were stronger than those of SAC. Flow cytometry revealed that SBC induced G2-phase arrest and apoptosis in SGC-7901 cells. Typical apoptotic morphological changes were observed by Hoechst 33258 dye assay. SBC-treatment dramatically induced the dissipation of mitochondrial membrane potential (ΔΨm), and enhanced the enzymatic activities of caspase-9 and caspase-3 whilst hardly affecting caspase-8 activity. Furthermore, Western blotting indicated that SBC-induced apoptosis was accompanied by up-regulation of the expression of p53, Bax and the down-regulation of Bcl-2. Taken together, this study suggested that SBC exerts cytotoxic activity involving activation of mitochondrial-dependent apoptosis through p53 and Bax/Bcl-2 pathways in human gastric cancer SGC-7901 cells.

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“…Yang's study found that the dose-dependent effects of both compounds were similar; the IC-50 values of EGCG and theaflavin were at 60 and 75 μg/mL respectively (equivalent to 130 -150 µM). It was previously shown that cytotoxicity to leukemic cells were induced by apoptosis [2]. Effects of tannic acid as an anti-cancer agent have not been fully explored.…”

Section: Discussionmentioning

confidence: 99%

“…Defined chemical constituents extracted from tea such as theaflavin, epigallocatechin gallate (EGCG) and epicathechin (EC) have been studied on various leukemia cell lines and showed good potential as chemopreventive or anti-cancer agentsexhibiting growth inhibitory effects in vitro [1][2][3]. Flavones found in plants and vegetables such as apigenin and quercetinare known to induce apoptosis in leukemia cells [4].…”

Section: Introductionmentioning

confidence: 99%

Identifying Combinatorial Growth Inhibitory Effects of Various Plant Extracts on Leukemia Cells through Systematic Experimental Design

Cheong¹,

Htay.²,

Tan³

et al. 2012

AJPS

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Plant extracts are widely studied for their anti-cancer and cancer preventive effects. In this study, we compared the leukemia growth inhibition effects of seven different plant extracts, theaflavin, epigallocatechin gallate (EGCG), epicathechin (EC), apigenin, quercetin, chrysin and tannic acid, in vitro using the K562 erythroleukemia cell line and application of the design of experiments (DoE) methodology. Our systematic approach enabled us to isolate the main factor contribution, two-factor interactions and produced interaction relationships and/or models to describe growth inhibitory effects of different plant extracts when they are used in combination. The results identified tannic acid as the most significant inhibitor in this group and had synergistic effects with EGCG at specific concentrations. The fitted model of their combined effects showed that the most potent combination is at low concentrations of tannic acid (10 -20 µM) and high concentrations of EGCG (80 -100 µM). We further showed that tannic acid induced both growth inhibition and apoptosis in K562 cells in ranges between 10 -100 µM. The polyphenol caused cell cycle arrest at G2-phase under the higher concentrations. In summary, use of DoE techniques effectively identified the most prominent inducer in this group of plant bioactive compounds and produced combinatorial bioactivity of various polyphenols and flavonoids over the entire range of concentrations under study. This study exemplifies the usefulness of DoE and serves as a guide in its utility for in vitro assessment of bioactivity in plant constituents.

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Induction of apoptosis in human leukemia cells by black tea and its polyphenol theaflavin

Cited by 79 publications

References 33 publications

Differentiation-associated alteration in sensitivity to apoptosis induced by (-)-epigallocatechin-3-O-gallate in HL-60 cells

Differentiation-associated alteration in sensitivity to apoptosis induced by (-)-epigallocatechin-3-O-gallate in HL-60 cells

S-benzyl-cysteine-mediated Cell Cycle Arrest and Apoptosis Involving Activation of Mitochondrial-dependent Caspase Cascade through the p53 Pathway in Human Gastric Cancer SGC-7901 Cells

Identifying Combinatorial Growth Inhibitory Effects of Various Plant Extracts on Leukemia Cells through Systematic Experimental Design

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