Induction of apoptosis in macrophages by cationic liposomes

Aramaki, Yukihiko; Takano, Shuhei; Tsuchiya, Satoshi

doi:10.1016/s0014-5793(99)01386-1

Cited by 89 publications

(68 citation statements)

References 25 publications

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“…[7][8][9] We first verified the suggestions using DOTAP-or DOTMA-containing liposomes without any cargo. When ROS generation was measured in a time-dependent manner, the increase in H 2 O 2 and LPO levels reached a plateau at approximately 40-60 min after treatment of DOTAP-liposomes (Fig.…”

Section: Cationic Lipid-specific Production Of Ros In Hepg2mentioning

confidence: 98%

“…6) In addition, cationic liposomes containing DOTAP or stearylamine have been suggested to induce ROS and concomitant apoptosis and/or toxicity in cells. [7][8][9] DOTMA also has the capacity for effective siRNA delivery with other cationic polymers (such as polyethylenimine) or receptor-targeted peptides as binary and ternary complexes. 10,11) In addition to siRNA, DOTMA-containing particles have been used for gene delivery vectors as lipo(poly) plexes or polyplexes.…”

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confidence: 99%

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Cargo-Free Nanoparticles Containing Cationic Lipids Induce Reactive Oxygen Species and Cell Death in HepG2 Cells

Yun

Bae

Ahn

2016

Biological & Pharmaceutical Bulletin

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These effects were dependent on the lipid concentration, surface density of cationic lipids, and particle size of NPs. However, neutral NPs consisting of 1,2-dioleoyl-3-phosphocholine did not elicit the effective ROS generation or cell death regardless of the lipid concentration and particle size. The present study suggests that DOTAP-and DOTMA-NPs are able to induce cancer cell death through production of ROS in the absence of any therapeutic cancer reagents. These results also provide a rational background for the design of delivery systems using cationic lipid-based NP formulations.

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Section: Cationic Lipid-specific Production Of Ros In Hepg2mentioning

confidence: 98%

mentioning

confidence: 99%

Cargo-Free Nanoparticles Containing Cationic Lipids Induce Reactive Oxygen Species and Cell Death in HepG2 Cells

Yun

Bae

Ahn

2016

Biological & Pharmaceutical Bulletin

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“…nonetheless, cationic lipids with a higher positive charge density are generally more toxic (14). moreover, some reports on toxicity have found that cationic liposomes with a monovalent positive charge (stearylamine) induced apoptosis and the production of reactive oxygen species (ROS) (15) in the raW264.7 (16) and mouse immature B (WEhi 231) cell lines (17). in in vivo toxicity studies of differently charged liposomes by intratracheal instillation in mice, lipofectamine was found to cause much greater toxicity than DOTAP in a dose-dependent manner, which was associated with ROS generation, while neutral and negative liposomes were not toxic at the relevant concentration (18).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity and antiapoptotic effect of Asparagus racemosus root extracts in human lung epithelial H460 cells

Kongkaneramit

Witoonsaridsilp

Peungvicha

et al. 2010

Experimental and Therapeutic Medicine

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Abstract. the present study examined the antioxidant activity and protective effect of extracts from Asparagus racemosus roots against lipofectamine-induced apoptosis. Five fractions from a successive extraction process ranging from non-polar to more polar solvents were obtained. the total saponin content as a marker in terms of diosgenin equivalent value of the root extracts was found to be in the range of 240-420 µg/ mg extract, with higher values for the ethanol and aqueous fractions. the antioxidant activity measured using the dpph method in terms of mean effective concentration (Ec 50 ) of the aqueous fraction was found to be 600 µg/ml as compared to 1.5 µg/ml of ascorbic acid. it is proposed that Asparagus racemosus root extracts effectively inhibit lipofectamineinduced apoptosis by their protective effect, and may serve as an advantageous alternative option for gene delivery.

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“…Some in vitro studies have been shown that CL induces cytotoxic effects or apoptosis in several cell types, for example, in phagocytic macrophages or immune cells. 11,12) In addition, other studies have demonstrated that some cationic lipid-based transfection agents induced geneexpression changes in human epithelial cells. 13) However, these investigations are limited owing to the fact that they were concerned only with material-related intrinsic cytotoxicity.…”

mentioning

confidence: 99%

Complex Formation with Plasmid DNA Increases the Cytotoxicity of Cationic Liposomes

Nguyen

Atobe

Barichello

et al. 2007

Biological & Pharmaceutical Bulletin

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Cationic liposomes (CL) are one of the most widely studied non-viral vectors for gene delivery. It is wellknown that CL induces cytotoxicity following lipofection. However, little is known regarding the mechanism involved in the cytotoxicity. In this study, the in vitro cytotoxicity of CL and its complex with pDNA (lipoplex) was investigated, and a part of the mechanism of induction as well. While free pDNA did not show any cytotoxicity, pDNA increased the cytotoxicity of CL via the formation of lipoplex. In addition, the lipoplex-induced cytotoxicity increased in a lipoplex dose-dependent manner, irrespective of the type of pDNA, cell line and the absence or presence of serum. An assay showed that apoptosis was largely induced by treatment with the lipoplex (lipofection), but not with CL alone, in the tested range of concentration of CL and pDNA. Furthermore, following treatment with lipoplexes, the cells exhibited the morphological features of apoptosis and DNA fragmentation. A cDNA microarray study showed that the lipofection up-regulated 45 genes related to apoptosis, transcription regulation and immune response. These results clearly indicate that pDNA in the lipoplex increases the cytotoxicity of CL as a result of inducing apoptosis. The fundamental principle for gene therapy is to deliver gene-based therapeutics to target cells for specific gene targeting with minimal cytotoxicity. Our results suggest the possibility that cytotoxicity induced by lipofection, accompanied by gene changes, could intrinsically exacerbate, attenuate or even mask the desired effects of gene-based therapy.

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Induction of apoptosis in macrophages by cationic liposomes

Cited by 89 publications

References 25 publications

Cargo-Free Nanoparticles Containing Cationic Lipids Induce Reactive Oxygen Species and Cell Death in HepG2 Cells

Cargo-Free Nanoparticles Containing Cationic Lipids Induce Reactive Oxygen Species and Cell Death in HepG2 Cells

Antioxidant activity and antiapoptotic effect of Asparagus racemosus root extracts in human lung epithelial H460 cells

Complex Formation with Plasmid DNA Increases the Cytotoxicity of Cationic Liposomes

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