Induction of apoptosis in yeast by L‐amino acid oxidase from the Malayan pit viper Calloselasma rhodostoma

Abstract

Here we report for the first time that L-amino acid oxidase (LAAO), a major component of snake venom, induces apoptosis in yeast. The causative agent for induction of apoptosis has been shown to be hydrogen peroxide, produced by the enzymatic activity of LAAO. However, the addition of catalase, a specific hydrogen peroxide scavenger, does not prevent cell demise completely. Intriguingly, depletion of leucine from the medium by LAAO and the interaction of LAAO with yeast cells are shown to be the major factors responsible for cell demise in the presence of catalase.

Isolation and biochemical, functional and structural characterization of a novel l-amino acid oxidase from Lachesis muta snake venom

“…This catalytic profile has been observed with LAAOs from Naja naja oxiana (Samel et al, 2008), Bothrops pirajai (Izidoro et al, 2006) and C. rhodostoma (Ande et al, 2008).…”

l-amino acid oxidase from Bothrops marajoensis causes nephrotoxicity in isolated perfused kidney and cytotoxicity in MDCK renal cells

“…The apoptotic effect of LAAOs from snake venoms has been reported by many authors, such as Lee et al (2014), Samel et al (2006), Zhang et al (2004), Alves et al (2008) and Ande et al (2008).…”

Protective Effects of a Rhodiola Crenulata Extract and Salidroside on Hippocampal Neurogenesis against Streptozotocin-Induced Neural Injury in the Rat

“…NSCs at P2 were seeded on poly-L-lysine-coated cultured plates and cultured with the plain culture medium alone (Normal Control group), H 2 O 2 at 1 mM [75] for four hours (H 2 O 2 Control group), STZ for four hours (STZ group), salidroside for twelve hours (Salidroside Blank group), salidroside for twelve hours followed by a four-hour incubation with STZ (Salidroside+STZ group), or catalase at 50 µg/ml [76] for twelve hours followed by a four-hour incubation with STZ (Catalase+STZ group). The culture medium was refreshed with the plain culture medium, and NSCs were further cultured for 8 h before various assays were performed.…”