Induction of apoptosis mediated by Fas receptor and activation of caspase-3 in MRL-+/+ or MRL-lpr/lpr murine oocytes

Guo, Maowu; Sato, Eimei; Li, Xiang; Mori, Etsuko; Saito, Shigeru; Mori, Tsuneatsu

doi:10.1017/s0967199402002034

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Further observations demonstrated that the apoptosis in wild-type mouse oocytes is achieved through the Fas receptor followed by the activation of caspase-3. In contrast, the aberrant expression and dysfunction of the mutant Fas receptor in lpr mouse oocytes were associated with an inability to activate caspase-3 and thus fail to induce nuclear DNA fragmentation 39 . However, effects of FasL (gld) mutations on oocytes have not been reported.…”

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confidence: 92%

Cumulus cells accelerate oocyte aging by releasing soluble Fas Ligand in mice

Zhu

Zhang

et al. 2015

Sci Rep

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Although previous studies have suggested that cumulus cells (CCs) accelerate oocyte aging by secreting soluble and heat-sensitive paracrine factors, the factors involved are not well characterized. Because Fas-mediated apoptosis represents a major pathway in induction of apoptosis in various cells, we proposed that CCs facilitate oocyte aging by releasing soluble Fas ligand (sFasL). In this study, we reported that when the aging of freshly ovulated mouse oocytes were studied in vitro, both the apoptotic rates of CCs and the amount of CCs produced sFasL increased significantly with the culture time. We found that oocytes expressed stable levels of Fas receptors up to 24 h of in vitro aging. Moreover, culture of cumulus-denuded oocytes in CCs-conditioned CZB medium (CM), in CZB supplemented with recombinant sFasL, or in CM containing sFasL neutralizing antibodies all showed that sFasL impaired the developmental potential of the oocytes whereas facilitating activation and fragmentation of aging oocytes. Furthermore, CCs from the FasL-defective gld mice did not accelerate oocyte aging due to the lack of functional FasL. In conclusion, we propose that CCs surrounding aging oocytes released sFasL in an apoptosis-related manner, and the released sFasL accelerated oocyte aging by binding to Fas receptors.

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confidence: 92%

Cumulus cells accelerate oocyte aging by releasing soluble Fas Ligand in mice

Zhu

Zhang

et al. 2015

Sci Rep

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“…In the bovine ovary, the Fas/FasL system may be regulated by gonadotropin-dependent mechanisms and may play a role during attrition, follicular regression, and atresia, because Fas antigen has been detected in oocytes from fetal and adult ovaries and in granulosa cells during the luteal phase (Guo et al, 2002;Kelkar et al, 2003). Fas ligand also mediates apoptosis in human granulose luteal cell cultures when these cells are pretreated with the Th1 cytokine, interferongamma (IFN-g) (Dzialo-Hatton et al, 2001;Huber et al, 2002).…”

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confidence: 99%