Induction of apoptosis of human colon carcinoma cells by an extract of &lt;i&gt;cordyceps militaris&lt;/i&gt; soaked in sweetpotato shochu

Sho, Cho; Sakao, Kozue; Ono, Tomomi; Takase, Yoshikazu; Iwai, Kenichi; Hou, De‐Xing

doi:10.6013/jbrewsocjapan.110.110

Cited by 1 publication

(6 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 21-day-infused spirits were further stored for 90, 15 and 240 days. All these spirits were concentrated to an extract and then eluted using 40 ethanol, which was proven as an effective solvent to obtain in vitro antitumor effect fra tions in our previous study [13,14]. As shown in Figure 1a, a time-dependent inhibition the proliferation of HCT-116 cells was observed in relation to the infusion times Cordyceps militaris in sweet potato shochu from 7 to 21 days.…”

Section: Influence Of Infusion Times and Storage Period On Hct-116 Ce...mentioning

confidence: 89%

“…To further identify Fraction D, 13 C-NMR, FT-IR spectral, and electrospray ionizationmass spectrometry (ESI-MS) analyses were performed. 13 ).…”

Section: Structural Analysis Of Fraction D Using 13 C-nmr Ft-ir and E...mentioning

confidence: 99%

“…To further identify Fraction D, 13 C-NMR, FT-IR spectral, and electrospray ionizationmass spectrometry (ESI-MS) analyses were performed. 13 ). Compared with published standard data, the 13 C-NMR chemical shifts of Fraction D were consistent with HEA (Figure 5b).…”

Section: Structural Analysis Of Fraction D Using 13 C-nmr Ft-ir and E...mentioning

confidence: 99%

“…13 ). Compared with published standard data, the 13 C-NMR chemical shifts of Fraction D were consistent with HEA (Figure 5b). The FT-IR spectra (Figure 5c) show a broad single absorption for O-H at 3291 cm −1 , with stretching vibrations.…”

Section: Structural Analysis Of Fraction D Using 13 C-nmr Ft-ir and E...mentioning

confidence: 99%

“…In previous studies, its shochu was concentrated and passed through a synthetic adsorbent resin column. The bioactive fraction, eluted with 40% ethanol, was further separated by HPLC to fractionate the cordycepin [13,14]. However, the other active ingredient in the spirits remains unidentified, although the typic cordycepin is confirmed.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Verification of In Vitro Anticancer Activity and Bioactive Compounds in Cordyceps Militaris-Infused Sweet Potato Shochu Spirits

Sakao,

Sho,

Miyata

et al. 2024

Molecules

Self Cite

View full text Add to dashboard Cite

Many liqueurs, including spirits infused with botanicals, are crafted not only for their taste and flavor but also for potential medicinal benefits. However, the scientific evidence supporting their medicinal effects remains limited. This study aims to verify in vitro anticancer activity and bioactive compounds in shochu spirits infused with Cordyceps militaris, a Chinese medicine. The results revealed that a bioactive fraction was eluted from the spirit extract with 40% ethanol. The infusion time impacted the inhibitory effect of the spirit extract on the proliferation of colon cancer-derived cell line HCT-116 cells, and a 21-day infusion showed the strongest inhibitory effect. Furthermore, the spirit extract was separated into four fractions, A-D, by high-performance liquid chromatography (HPLC), and Fractions B, C, and D, but not A, exerted the effects of proliferation inhibition and apoptotic induction of HCT-116 cells and HL-60 cells. Furthermore, Fractions B, C, and D were, respectively, identified as adenosine, cordycepin, and N6-(2-hydroxyethyl)-adenosine (HEA) by comprehensive chemical analyses, including proton nuclear magnetic resonance (1H-NMR), Fourier transform infrared spectroscopy (FT-IR), and electrospray ionization mass spectrometry (ESI-MS). To better understand the bioactivity mechanisms of cordycepin and HEA, the agonist and antagonist tests of the A3 adenosine receptor (A3AR) were performed. Cell viability was suppressed by cordycepin, and HEA was restored by the A3AR antagonist MR1523, suggesting that cordycepin and HEA possibly acted as agonists to activate A3ARs to inhibit cell proliferation. Molecular docking simulations revealed that both adenosine and cordycepin bound to the same pocket site of A3ARs, while HEA exhibited a different binding pattern, supporting a possible explanation for the difference in their bioactivity. Taken together, the present study demonstrated that cordycepin and HEA were major bioactive ingredients in Cordyceps militaries-infused sweet potato shochu spirits, which contributed to the in vitro anticancer activity.

show abstract

Section: Influence Of Infusion Times and Storage Period On Hct-116 Ce...mentioning

confidence: 89%

“…To further identify Fraction D, 13 C-NMR, FT-IR spectral, and electrospray ionizationmass spectrometry (ESI-MS) analyses were performed. 13 ).…”

Section: Structural Analysis Of Fraction D Using 13 C-nmr Ft-ir and E...mentioning

confidence: 99%

Section: Structural Analysis Of Fraction D Using 13 C-nmr Ft-ir and E...mentioning

confidence: 99%

Section: Structural Analysis Of Fraction D Using 13 C-nmr Ft-ir and E...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Verification of In Vitro Anticancer Activity and Bioactive Compounds in Cordyceps Militaris-Infused Sweet Potato Shochu Spirits

Sakao,

Sho,

Miyata

et al. 2024

Molecules

Self Cite

View full text Add to dashboard Cite

show abstract

Induction of apoptosis of human colon carcinoma cells by an extract of <i>cordyceps militaris</i> soaked in sweetpotato shochu

Cited by 1 publication

References 9 publications

Verification of In Vitro Anticancer Activity and Bioactive Compounds in Cordyceps Militaris-Infused Sweet Potato Shochu Spirits

Verification of In Vitro Anticancer Activity and Bioactive Compounds in Cordyceps Militaris-Infused Sweet Potato Shochu Spirits

Contact Info

Product

Resources

About