Induction of aromatic amino acids and phenylpropanoid compounds in Scrophularia striata Boiss. cell culture in response to chitosan-induced oxidative stress

Kamalipourazad, Maryam; Sharifi, Mohsen; Maivan, H Zare; Behmanesh, Mehrdad; Chashmi, Najmeh Ahmadian

doi:10.1016/j.plaphy.2016.06.034

Cited by 61 publications

(26 citation statements)

References 45 publications

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“…(Ramirez-Estrada et al, 2016). It is reported that chitosan elicitation can induce ROS overproduction, perturb the intracellular redox equilibrium and ultimately result in oxidative stress in S. striata cell cultures (Kamalipourazad et al, 2016). In this work, chitosan-elicited root tissues exhibited browning color as compared with control ( Fig.…”

Section: Profiles Of Eight Flavonoids In Ithrcs Under the Appropriatementioning

confidence: 51%

“…artemisinin in Artemisia annua L. (Lei et al, 2011), curcumin in Curcuma longa L. (Sathiyabama et al, 2016), echinacoside in cell suspension cultures of Scrophularia striata Boiss. (Kamalipourazad et al, 2016), hydrolysable tannin in cell suspension cultures of Phyllanthus debilis Klein ex Willd. (Malayaman et al, 2017), withanolides in adventitious root cultures of Withania somnifera (L.) Dunal (Sivanandhan et al, 2012), and plumbagin in adventitious root cultures of Plumbago indica L. (Jaisi and Panichayupakaranant, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…It is noteworthy that the toxicity of chitosan is very close to that of salt or sugar (Singla and Chawla, 2001). In view of costs and product safety, utilization of chitosan as a cost-effective and nontoxic elicitor for enhancing phytochemical production in plant in vitro cultures is strongly recommended (Jaisi and Panichayupakaranant, 2017;Kamalipourazad et al, 2016;Malayaman et al, 2017;Sivanandhan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Chitosan elicitation of Isatis tinctoria L. hairy root cultures for enhancing flavonoid productivity and gene expression and related antioxidant activity

Jiao

Gai

Wang

et al. 2018

Industrial Crops and Products

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Elicitation for phytochemical enhancement via cost-effective elicitors can overcome the limitation of commercial application faced by plant cell and organ culture technology. Chitosan is a natural, low-cost, and nontoxic elicitor that can trigger plant defense responses with the concomitant enhancement in phytochemical biosynthesis. In this work, the elicitation of Isatis tinctoria L. hairy root cultures by chitosan was conducted to enhance the production of pharmacologically active flavonoids. In comparison with control (2.31 ± 0.29 mg/g DW), a 7.08-fold enhancement of total flavonoids (16.35 ± 0.88 mg/g DW) was achieved in 24 day-old I. tinctoria hairy root cultures elicited by 150 mg/L chitosan for 36 h. Interestingly, the multiple hydroxyl-substituted flavonoids (rutin, quercetin, isorhamnetin, and isoliquiritigenin) were noticed to increase significantly in chitosan-elicited I. tinctoria hairy root cultures. Moreover, the transcription of associated genes involved in flavonoid biosynthesis pathway was significantly up-regulated underlying chitosan elicitation, among which chalcone synthase and flavonoid 3′-hydroxylase might play an important role in flavonoid enhancement. Additionally, extracts from chitosan-elicited I. tinctoria hairy root cultures exhibited higher antioxidant activities with lower IC 50 values as compared with control. Overall, a cost-effective strategy via the simple chitosan elicitation is provided here to enhance the production of high-added value flavonoids in I. tinctoria hairy root cultures, which paves the way toward the successful commercialization of this in vitro culture system in the future.

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Section: Profiles Of Eight Flavonoids In Ithrcs Under the Appropriatementioning

confidence: 51%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chitosan elicitation of Isatis tinctoria L. hairy root cultures for enhancing flavonoid productivity and gene expression and related antioxidant activity

Jiao

Gai

Wang

et al. 2018

Industrial Crops and Products

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“…Meanwhile, phenylpropanoid pathway is another most important secondary metabolism pathways and defense responses in higher plants [15,16]. Phenylalanine ammonia-lyase (PAL) is the rst enzyme in the phenylpropanoid pathway, participating in the formation of a series of structural and defensive lignin and phenolic compounds [17][18][19][20]. Furthermore, PAL gene has been widely studied in participating in plant growth, development and defense systems [21][22][23], such as the upregulated expression of PAL gene in plants that can help plant to develop resistance to phytopathogens infection [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of different cultivars of Cucurbita pepo in resistance to Podosphaera xanthii infection through improvement of antioxidative defense system and gene expression

Zhang¹,

Xu²,

Liu³

2020

Preprint

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Background: Powdery mildew is one of the world’s most destructive diseases of cucurbit and the major cause of losses in its production worldwide. A number of strategies have been developed and applied to discover some suitable and alternative safe methods to manage the powdery mildew disease occurrence, but little information is regarding to screen of resistant pumpkins (Cucurbita pepo L.) germplasm and explore the mechanisms of their preventing the disease occurrence at physiological, biochemical, and molecular levels. Therefore, we evaluated and determined the ability and mechanisms of two commercial pumpkin cultivars in resistance to Podosphaera xanthii infection. Results: Compared with mock-inoculated seedlings, small and sparse spots were observed on the cultivar of Sixing F1 leaves at the 13th day after inoculation with P. xanthii, whereas a large number of disease spots or a layer of white powdery mildew were observed on the surface of Jin12 F1 leaves. Increased the inoculation time (7, 9, 11 and 13 days) significantly and continuously increased the disease incidence and index of pumpkin seedlings after inoculation. The disease incidence and index of Jin12 F1 were significantly higher than the cultivar of Sixing F1. At Day 13, the disease incidence and index of Jin12 F1 were 80.0% and 72.6, whereas the cultivar of Sixing F1 was 22.3% and 17.7, respectively. The contents of H2O2, MDA, lignin and total phenolics in the leaves of Sixing F1 and Jin12 F1 were markedly accelerated after inoculation with P. xanthii. However, the cultivar of Sixing F1 exhibited less reactive oxygen species (ROS) accumulation, a lower rate of lipid peroxidation and higher level of lignin and total phenolics contents after inoculation, whereas the cultivar of Jin12 F1 exhibited higher level of ROS accumulation and rates of lipid peroxidation, and lower level of lignin and total phenolics contents. Higher activity and transcript level of antioxidant enzymes and gene of PAL expression were observed on different tissues of both cultivars after inoculated with P. xanthii. Compared with pumpkin seedlings that were inoculated with sterile water but not P. xanthii, the level of PAL activity and gene expression in leaves, petioles and stems of Sixing F1 and Jin12 F1 were upregulated and increased significantly at different time points after inoculation. The enhancement expression of PAL activity and gene in different tissues of Sixing F1 was significantly higher than Jin12 F1, and higher in leaves, lower in petioles and stems. Conclusions: Our results indicate that the cultivar of Sixing F1 exhibited the highest ability in resistance to P. xanthii infection in comparison to Jin12 F1, and one novel possible mechanism is related to the cultivars in resistance to P. xanthii infection by activating and enhancing the antioxidative defense system and gene expression to prevent the pathogens infection.

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“…Phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) is the first enzyme in the phenylpropanoid pathway and catalyzes the conversion of L-phenylalanine to trans-cinnamic acid by nonoxidative deamination [1][2][3]. Furthermore, PAL is the first key enzyme in the phenylpropanol pathway, participating in the formation of a series of structural and defensive phenolic compounds, such as lignin, phenolic acid and hydroxybenzoic acid, flavonoids and stilbene in plants [4].…”

Section: Introductionmentioning

confidence: 99%

Expression patterns of two pal genes of Pleurotus ostreatus across developmental stages and under heat stress

Hou

Wang

et al. 2019

Preprint

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Background: Phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) is the first key enzyme in the phenylpropanoid pathway. The pal gene has been widely studied in plants and participates in plant growth, development and defense systems. However, in P leurotus ostreatus , the biological functions of pal during organismal development and exposure to abiotic stress have not been reported. Results: In this study, we cloned and characterized the pal1 (2232 bp) and pal2 (2244 bp) genes from the basidiomycete P. ostreatus CCMSSC 00389. The pal1 and pal2 genes are interrupted by 6 and 10 introns, respectively, and encode proteins of 743 and 747 amino acids, respectively. Furthermore, prokaryotic expression experiments showed that PAL enzymes catalyzed the conversion of L-phenylalanine to trans-cinnamic acid. The function of pal1 and pal2 was determined by constructing overexpression (OE) and RNA interference (RNAi) strains. The results showed that the two pal genes had similar expression patterns during different developmental stages. The expression of pal genes was higher in the reproductive growth stage than in the vegetative growth stage . And the interference of pal1 and pal2 delayed the formation of primordia. The results of heat stress assays showed that the RNAi- pal1 strains had enhanced mycelial tolerance to high temperature, while the RNAi- pal2 strains had enhanced mycelial resistance to H 2 O 2 . Conclusions: These results indicate that two pal genes may play a similar role in the development of P. ostreatus fruiting bodies, but may alleviate stress through different regulatory pathways under heat stress.

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Induction of aromatic amino acids and phenylpropanoid compounds in Scrophularia striata Boiss. cell culture in response to chitosan-induced oxidative stress

Cited by 61 publications

References 45 publications

Chitosan elicitation of Isatis tinctoria L. hairy root cultures for enhancing flavonoid productivity and gene expression and related antioxidant activity

Chitosan elicitation of Isatis tinctoria L. hairy root cultures for enhancing flavonoid productivity and gene expression and related antioxidant activity

Mechanisms of different cultivars of Cucurbita pepo in resistance to Podosphaera xanthii infection through improvement of antioxidative defense system and gene expression

Expression patterns of two pal genes of Pleurotus ostreatus across developmental stages and under heat stress

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