Induction of auxin biosynthetic enzymes by jasmonic acid and in clubroot diseased Chinese cabbage plants

Abstract

oxidizing enzyme (TrpOxE), which catalyzes the first step in Nitrilase (NIT) and myrosinase are important enzymes for tryptophan-dependent auxin biosynthesis in Brassicaceae, and auxin biosynthesis in Brassicaceae, which is increased during clubroot disease. Therefore, NIT and myrosinase levels during nitrilase were enhanced after treatment with jasmonic acid (JA) and methyl jasmonate. Similarly, the amount of myrosi-club development and possible regulation mechanisms were nase mRNA was increased by JA. During clubroot disease the investigated. In addition, the occurrence of different nitrilase isoforms in Chinese cabbage has been shown. Nitrilase activity endogenous concentration of JA increased in infected roots -5 weeks after inoculation. From our results it can be was enhanced in infected roots during later stages of club development (35-42 days after inoculation). However, no concluded that: (1) de novo indole-3-acetic acid (IAA) biosyndifferences in nitrilase mRNA levels between infected and thesis plays a role for symptom development of clubroot healthy roots were found during symptom development. My-disease in Brassicaceae during later developmental stages; and(2) JA which increased during club development, may be rosinase expression was increased in clubbed roots at slightly involved in the up-regulation of three enzymes important for earlier time points (28 days after inoculation) and also at later time points during infection. The activities of tryptophan IAA synthesis.

Auxin increases the InJMT expression and the level of JAMe – inhibitor of flower induction in Ipomoea nil

“…This provides an explanation that the mechanism of flower inhibition by auxin is dependent on JAMe. Indeed, the interdependent action of both hormones in the regulation of other physiological processes was also observed during root, stamen, and filament growth [32,53,54]. This work confirms that InJMT involved in JAs metabolism in I. nil demonstrates a high homology to genes identified in other plant species.…”

The Arabidopsis thaliana Isogene NIT4 and Its Orthologs in Tobacco Encode β-Cyano-l-alanine Hydratase/Nitrilase

“…We propose to reserve the gene name NIT4 for Ala(CN) hydratases/nitrilases. Nitrilases from other plants belonging to the NIT4 family should therefore also be called NIT4 independent of the total number of NIT genes present, as previously done for B. campestris (15), tobacco (8), and rice.…”

“…Genomic or cDNA sequences of nitrilases are now known from A. thaliana, two Brassica species (B. campestris and Brassica oleracea) (15,16), tobacco (N. tabacum) (17), and rice (O. sativa, GenBank TM accession number AB027054). In addition several expressed sequence tag clones exist, which encode nitrilase-like proteins.…”

“…Are not Restricted to BrassicaceaePartial or complete cDNA or genomic sequences of nitrilases are known from several plant species like A. thaliana (1-3), Brassica campestris (Chinese cabbage) (15,16), Lotus japonicus (GenBank TM accession number AW720658), N. tabacum (tobacco) (8), and Oryza sativa (rice) (GenBank TM accession number AB027054). By comparing the homologies between these nitrilases ( Fig.…”

Influence of abiotic stress signals on secondary metabolites in plants

“…MeJA can also promote the biosynthesis of endogenous IAA in plants. 95 Exogenous application of jasmonates greatly stimulated the biosynthesis of a wide range of secondary metabolites in cell suspension cultures, and in intact plants. 94 MeJA induced anthocynin accumulation was reported in Arabidopsis thaliana, 96 strawberry fruits, 97 Vaccinium pahalae, 98 Vitis vinifera 99 and tulip leaves.…”