Induction of basal lamina formation in epidermal cell cultures in vitro

Mann, Patricia R.; Constable, Hilary

doi:10.1111/j.1365-2133.1977.tb07138.x

Cited by 28 publications

(10 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several reports in the literature suggest that epithelial ceils will only form hemidesmosomes if they are grown on substrata coated with components of the extracellular matrix (Mann and Constable, 1977;Chapman and Eady, 1985;Eady, 1988). For example, hemidesmosomes are formed by epithelial cells within 3-5 d when such cells are cultured on collagen gels but hemidesmosome assembly is not observed in cells maintained on bare plastic (Mann and Constable, 1977;Chapman and Eady, 1985;Eady, 1988). In contrast, Shellswell et al 0984) have shown that embryonic corneal cells form electron densities along their basal surface when cultured on tissue culture plastic.…”

Section: Discussionmentioning

confidence: 99%

Formation of hemidesmosomes in vitro by a transformed rat bladder cell line.

Riddelle

Green

Jones

1991

The Journal of Cell Biology

View full text Add to dashboard Cite

Abstract. Two hemidesmosomal plaque components of 230 and 180 kD have recently been characterized using autoantibodies in the serum samples of bullous pemphigoid (BP) patients (Klatte, D. H., M. A. Kurpakus, K. A. Grelling, and J. C. R. Jones. 1989, J. Cell Biol. 109:3377-3390). These BP autoantibodies generate the type of staining patterns that one would predict for formed hemidesmosomes, i.e., a punctate staining pattern towards the substratum; in <50% of various primary epithelial and transformed epidermal cell lines even when such cells are maintained in culture for prolonged periods. In contrast, affinitypurified human autoantibodies against the 230-kD hemidesmosomal plaque component generate intense immunofluorescence staining along the region of cell-substratum interaction in the rat bladder tumor cell line 804G maintained on uncoated glass coverslips. This pattern is distinct from that observed in the 804G cells using an antibody preparation directed against vinculin, a component of adhesion plaques. Ultrastructural analyses of the 804G cells reveals that hemidesmosome-like structures occur along the basal surface of cells where they abut the substratum. These structures are present in 804G cells maintained in culture in reduced levels of Ca 2÷ and are recognized by autoantibodies directed against the 230-kD hemidesmosomal plaque component as determined by immunogold ultrastructural localization.To study hemidesmosome appearance in this cell line, 804G ceils were trypsinized and then allowed to readhere to glass coverslips. In rounded, unattached 804G cells, hemidesmosome-like plaque structures occur along the cell surface. These structures are recognized by the 230-kD autoantibodies. At 1 h after plating, hemidesmosomes are observed along the substratum attached surface of cells. Protein synthesis is not required for the appearance of these hemidesmosomes. Within 4 h of plating, autoantibody staining and hemidesmosomes appear towards the cell periphery. Subsequently, the polypeptide recognized by the BP autoantibodies becomes concentrated in the perinuclear region, where there are numerous hemidesmosomes. We propose that the hemidesmosomes in 804G cells are involved in cell-substratum adhesion. We discuss possible mechanisms of assembly of hemidesmosomes in the 804G cells. Indeed, the 804G cells should prove an invaluable cell line for the biochemical and molecular dissection of hemidesmosome structure, function, and assembly.

show abstract

Section: Discussionmentioning

confidence: 99%

Formation of hemidesmosomes in vitro by a transformed rat bladder cell line.

Riddelle

Green

Jones

1991

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…In two other in vitro studies, elements of the BM were synthesized by culturing epidermal cell suspensions on a collagen gel, i.e., in the absence of a dermal layer) (Mann and Constable 1977;Taniguchi and Hirone 1983). Hemidesmosomes were synthesized along the plasma membrane of epidermal cells at the interface with collagen gel and synthesis of a continuous lamina densa was eventually observed; however, anchoring fibrils were not reported (Taniguchi and Hirone 1983).…”

Section: Synthesis Of a Skin Bmmentioning

confidence: 99%

Tissue and Organ Regeneration in Adults

Yannas

2015

108

100

View full text Add to dashboard Cite

“…Previous reports have demonstrated that extracellular matrix materials can modulate basal lamina production . For example, culturing epithelial cells on collagen gels was found to promote the deposition of basal lamina-like structures (9,24,41). In addition, Garbi and Wollman (18) have shown that the addition of soluble laminin to explants of thyroid epithelium induces basal lamina formation, which otherwise fails to develop .…”

Section: Ultrastructure Of a Subendothelial Matrix Deposited By Cellsmentioning

confidence: 99%

Basal lamina formation by cultured microvascular endothelial cells.

Kramer¹,

Bensch²,

Davison³

et al. 1984

The Journal of Cell Biology

View full text Add to dashboard Cite

The production of a basal lamina by microvascular endothelial cells (MEC) cultured on various substrata was examined . MEC were isolated from human dermis and plated on plastic dishes coated with fibronectin, or cell-free extracellular matrices elaborated by fibroblasts, smooth muscle cells, corneal endothelial cells, or PF HR9 endodermal cells. Examination of cultures by electron microscopy at selected intervals after plating revealed that on most substrates the MEC produced an extracellular matrix at the basal surface that was discontinuous, multilayered, and polymorphous . Immunocytochemical studies demonstrated that the MEC synthesize and deposit both type IV collagen and laminin into the subendothelial matrix . When cultured on matrices produced by the PF HR9 endodermal cells MEC deposit a subendothelial matrix that was present as a uniform sheet which usually exhibited lamina raraand lamina densa-like regions . The results indicate that under the appropriate conditions, human MEC elaborate a basal lamina-like matrix that is ultrastructurally similar to basal lamina formed in vivo, which suggests that this experimental system may be a useful model for studies of basal lamina formation and metabolism.Capillary endothelium lie on a basal lamina that has a number of important functions. Not only does it provide the substratum for endothelial cell attachment and tissue organization (52), but it also has a role in blood vessel permeability (15), in the initiation of blood clotting (2), and provides a barrier to cellular migration, the violation of which has importance for angiogenesis (17), leukocyte emigration (55), and tumor metastasis (35,38). Alterations in vascular basal lamina have been implicated in a number of pathological states, including diabetes mellitus, thrombogenesis, atherosclerosis, and renal failure (3,15,28,53).The vascular basal lamina is a complex structure . Because of difficulties in isolating sufficient quantities of purified material, biochemical studies of its molecular composition and structure have been limited . It is known, however, that vascular basal lamina is composed of a number of macromolecules including type IV collagen, proteoglycans, and glycoproteins (reviewed in 32, 37, 50). Studies on the synthesis of basal lamina macromolecules and their assembly into mature basal lamina by endothelia has been even more difficult. Recent advances in the isolation and long term culture of microvascular endothelial cells (3,10,17,40) provides the opportunity for the study of these events. 692

show abstract

Induction of basal lamina formation in epidermal cell cultures in vitro

Cited by 28 publications

References 12 publications

Formation of hemidesmosomes in vitro by a transformed rat bladder cell line.

Formation of hemidesmosomes in vitro by a transformed rat bladder cell line.

Tissue and Organ Regeneration in Adults

Basal lamina formation by cultured microvascular endothelial cells.

Contact Info

Product

Resources

About