Induction of Bone in Composites of Osteogenin and Porous Hydroxyapatite in Baboons

Ripamonti, Ugo; Ma, Shu-Shan; Reddi, A. Hari

doi:10.1097/00006534-199204000-00026

Cited by 18 publications

(23 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous experiments, partially converted coral-derived HA/ CC substrata with a ''coating'' of 2 mm depth of hydroxyapatite on the porous resorbable framework of calcium carbonate failed to induce the intrinsic induction of bone morphogenesis as evaluated 30 and 90 days after implantation in the rectus abdominis muscle of P. ursinus [22]. These partially converted HA/CC constructs did not induce bone formation even when reconstituted with highly purified naturally derived bone morphogenetic/osteogenic proteins (BMPs/OPs) [22].…”

Section: Discussionmentioning

confidence: 93%

“…These partially converted HA/CC constructs did not induce bone formation even when reconstituted with highly purified naturally derived bone morphogenetic/osteogenic proteins (BMPs/OPs) [22]. Limited induction of bone formation was also seen in coral-derived constructs of different ratios of calcium phosphate to calcium carbonate with or without highly purified naturally derived BMPs/OPs implanted in non-healing calvarial defects of P. ursinus [31].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated that the collagen-derived P15 peptide enhances cell attachment to inorganic bone mineral, providing cell orienting signals enhancing the biomimetic environment of substrata [20,21]. In previous experiments, partially converted hydroxyapatite/calcium carbonate constructs did not induce bone formation by induction, even when implanted after reconstitution with highly purified naturally derived bone morphogenetic/osteogenic proteins (BMPs/OPs) [22].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The induction of bone formation by coral-derived calcium carbonate/hydroxyapatite constructs

et al. 2009

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The induction of bone formation by coral-derived calcium carbonate/hydroxyapatite constructs

et al. 2009

Self Cite

View full text Add to dashboard Cite

“…HA has already been investigated as carrier for rhBMP-2 in rabbit skull for its effect on progression of bone formation. 11,12 However, the coating of solid HA implants with rhBMP-2 has never been studied in a cortical bone defect animal model before. The purpose of this study was to test the hypothesis whether the coating of solid HA implants with rhBMP-2 leads to faster new bone formation and bony incorporation of the composite material compared with the uncoated implant in a cortical tibia shaft model in miniature pigs.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of bone formation in hydroxyapatite implants by rhBMP‐2 coating

et al. 2008

View full text Add to dashboard Cite

The combination of hydroxyapatite (HA) implants serving as osteoconductive scaffold with growth factors is an interesting approach for the improvement of bone defect healing. The purpose of this study was to test whether recombinant human bone morphogenetic protein-2 (rhBMP-2) coating of solid HA-implants improves bone formation in a cortical bone defect. Cylindrical trephine mill defects (diameter: 9.8 mm, depth: 10 mm) were created into the cortical tibia shaft of minipigs and subsequently filled either by plain HA cylinders (Endobon) or by rhBMP-2-coated HA cylinders. Fluorochrome labeling for the evaluation of time-dependent bone formation was done on days 8, 9, and 10 postsurgery with tetracyclin-100, at days 25 and 30 with alizarin-komplexon, and finally on days 32, 37, 73, and 79 with calcein green. Twelve weeks after implantation, the tibiae were harvested and were prepared for standard histological staining, fluorochrome analysis, and histomorphometry. Coating of HA implants with rhBMP-2 led to significant enhanced new bone formation of 84.7% (+/-4.6%) of the implant area with almost complete bony incorporation compared with only 27.7% (+/-8.5%) in the uncoated HA implants (p = 0.028). In both types of implants, osteoconduction of HA led to bone ingrowth of the surrounding host bone into the implants. However, only rhBMP-2-coated implants showed multitopic de novo bone formation reflecting the osteoinductive properties of rhBMP-2 in all areas of the HA implant. This study showed that the coating of HA ceramic implants with rhBMP-2 can significantly enhance new bone formation attributable to its osteoinductive effects.

show abstract

“…The extent of resorption and/or dissolution of biphasic calcium phosphate bioceramics has been essentially investigated in rodent, lagomorph and canine models, and very limited studies have been reported using non-human primate species [17,18]. After implantation of coral-derived hydroxyapatites with different ratios of calcium phosphate to calcium carbonate with or without highly purified naturally-derived BMPs/OPs [2], results showed limited if any biomatrix resorption over time in heterotopic rectus abdominis sites [17]. In orthotopic sites, both control and BMPs/OPs-treated specimens showed variable but often extensive resorption and dissolution of the implanted porous substrata [18].…”

mentioning

confidence: 99%

The induction of bone formation by smart biphasic hydroxyapatite tricalcium phosphate biomimetic matrices in the non‐human primate Papio ursinus

Ripamonti

Richter

Nilen

et al. 2008

J Cellular Molecular Medi

View full text Add to dashboard Cite

Long-term studies in the non-human primate Chacma baboon Papio ursinus were set to investigate the induction of bone formation by biphasic hydroxyapatite/p-tricalcium phosphate (HA/β-TCP) biomimetic matrices. HA/β-TCP biomimetic matrices in a pre-sinter ratio (wt%) of 40/60 and 20/80, respectively, were sintered and implanted in the rectus abdominis and in calvarial defects of four adult baboons. The post-sinter phase content ratios were 19/81 and 4/96, respectively. Morphological analyses on day 90 and 365 showed significant induction of bone formation within concavities of the biomimetic matrices with substantial bone formation by induction and resorption/dissolution of the implanted matrices. One year after implantation in calvarial defects, 4/96 biphasic biomimetic constructs showed prominent induction of bone formation with significant dissolution of the implanted scaffolds. The implanted smart biomimetic matrices induce de novo bone formation even in the absence of exogenously applied osteogenic proteins of the transforming growth factor-β(TGF-β) superfamily. The induction of bone formation biomimetizes the remodelling cycle of the cortico-cancellous bone of primates whereby resorption lacunae, pits and concavities cut by osteoclastogenesis are regulators of bone formation by induction. The concavities assembled in HA/β-TCP biomimetic bioceramics are endowed with multifunctional pleiotropic self-assembly capacities initiating and promoting angiogenesis and bone formation by induction. Resident mesenchymal cells differentiate into osteoblastic cell lines expressing, secreting and embedding osteogenic soluble molecular signals of the TGF-β superfamily within the concavities of the biomimetic matrices initiating bone formation as a secondary response.

show abstract

Induction of Bone in Composites of Osteogenin and Porous Hydroxyapatite in Baboons

Cited by 18 publications

References 0 publications

The induction of bone formation by coral-derived calcium carbonate/hydroxyapatite constructs

The induction of bone formation by coral-derived calcium carbonate/hydroxyapatite constructs

Enhancement of bone formation in hydroxyapatite implants by rhBMP‐2 coating

The induction of bone formation by smart biphasic hydroxyapatite tricalcium phosphate biomimetic matrices in the non‐human primate Papio ursinus

Contact Info

Product

Resources

About