Induction of Branch Retinal Vein Occlusion by Photodynamic Therapy with Rose Bengal in a Rabbit Model

Zhou, Xiaoxu; Song, Yanping; Zhao, Yutong; Wu, Jianhui

doi:10.5772/27623

Cited by 3 publications

(7 citation statements)

References 17 publications

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“…Photodynamic coagulation is another method that has been used to induce BRVO. [93][94][95]112,119,148,149 This method involves light illumination using a slitlamp and a contact lens, or an endo illuminator in combination with vitrectomy aiming at selected retinal vein or veins, with care not to damage retinal arteries, for a duration ranging between 6 and 20 minutes until evidence of venous occlusion is observed. [93][94][95]112,119,148,149 Photosensitizers, such as Rose Bengal, 93-95,112,119,148,149 sodium fluorescein, 119 and NPe6, 82 have been used in different doses depending on the species used to facilitate thrombus formation.…”

Section: Methods Of Inducing Rvomentioning

confidence: 99%

“…85,89,92,96,104,105,120,121,124,129,133,139,175,179,180,183,187,189,192,221,222 Recanalization was observed in 0% to 100% of eyes of BRVO models 1 to 14 days following induction. 70,80,85,89,92,93,[95][96][97]104,105,110,112,126,129,132,137,186,221 Collateral vessels were prominent 5 to 14 days following establishment of the RVO 92,129,179,180,192 ( Tables 7, 8).…”

Section: Clinical and Histopathologic Features Of Rvo Modelsmentioning

confidence: 98%

“…Several animal species have been used to study RVO, including rodents, rabbits, [101][102][103][104][105][106][107][108][109][110][111][112][113][114] cats, [115][116][117][118][119][120][121][122][123][124] dogs, [125][126][127] pigs, and nonhuman primates 82,111,129, (Tables 1, 2). Each of these species has its own size and anatomic advantages, but also ethical challenges and cost implications; these have been summarized in Table 3.…”

Section: Speciesmentioning

confidence: 99%

“…96,133,221 The areas of capillary nonperfusion persisted during the follow-up, which ranged between 1 and 20 weeks, despite reperfusion. 70,80,85,89,92,96,97,132,133,150,175,179,189,[191][192][193]221,222 Reperfusion in these models was either by recanalization/reopening of the occluded vessels in some or all eyes, 70,80,85,89,90,92,93,[95][96][97]104,105,110,112,115,120,126,129,132,133,137,186,187,222 or development of collateral vessels. 85,89,92,96,104...…”

Section: Clinical and Histopathologic Features Of Rvo Modelsmentioning

confidence: 99%

“…132,133,222 Photoreceptor cell loss was observed in 67% of eyes at 3 months following the occlusion 180 Damage to the photoreceptors was reported in photodynamic-induced thrombosis in rats within 2 days of the occlusion, which was most likely related to the photodynamic therapy itself rather than the result of ischemia. 112 Unspecified RPE changes were reported 4 weeks to 3 months following occlusion in laser-induced BRVO nonhuman primate models. 152,180,192 Functional Changes.…”

Section: Clinical and Histopathologic Features Of Rvo Modelsmentioning

confidence: 99%

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Animal Models of Retinal Vein Occlusion

Khayat

Lois

Williams

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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Section: Methods Of Inducing Rvomentioning

confidence: 99%

Section: Clinical and Histopathologic Features Of Rvo Modelsmentioning

confidence: 98%

Section: Speciesmentioning

confidence: 99%

Section: Clinical and Histopathologic Features Of Rvo Modelsmentioning

confidence: 99%

Section: Clinical and Histopathologic Features Of Rvo Modelsmentioning

confidence: 99%

See 3 more Smart Citations

Animal Models of Retinal Vein Occlusion

Khayat

Lois

Williams

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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Clinical and Immunological Analysis of Retinal Pigment Epithelium Transplantation Derived from Induced Pluripotent Stem Cells under Pharmacological Immunosuppression in Rabbits

Neroeva,

Balatskaya,

Brilliantova

et al. 2024

Oftalʹmologiâ

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justification. Degenerative retinal diseases associated with retinal pigment epithelium damage are untreated and lead to irreversible vision loss. The most promising approach nowadays is considered to be the transplantation of retinal pigment epithelium differentiated from pluripotent stem cells (PSC-RPE). When testing biomedical cellular products, special requirements are placed on the animal model, which should simulate pathological changes, such as in humans, and have sufficient immunological tolerance to xenogenic human cells, enabling them to function fully. For this purpose, lines of immuno-modified or immunodeficiency animals are used, usually small rodents (rats and mice), but the small size and structure of their eyes do not allow manipulation in vitreoretinal interventions in humans. An alternative method of preventing xenotransplantation rejection in large models is pharmacological immunosuppression, under which transplanted PSC-RPE may have biological effects. However, targeted studies of the effectiveness of PSC-RPE transplantation with use of immunosuppressive therapy have not been conducted in our country, and the data of a few foreign works devoted to this problem are contraversial.Purpose. Study of immunological reactivity in transplantation of IPSC-RPE under combined immunosuppressive therapy in rabbit experiment.Materials and methods. The study was performed on 27 male rabbits of New Zealand albino breed, of which 24 animals underwent subretinal transplantation of induced pluripotent stem cells of retinal pigment epithelium: in the I group (12 eyes) intervention was performed in the healthy eye; in group II (12 eyes) — in the area of pre-modeled atrophy of retinal pigment epithelium. Recipients were given combined immunosuppressive therapy. Control Group — 3 intact rabbits (6 eyes). The observation period was 14, 28 and 60 days. Post-transplant evaluation was carried out using standard and specialized ophthalmological examination methods. The determination of cytokines of the immune response IL-2, IL-6, immunosuppressive factors TGF-β1, TGF-β2, TSP-1 in the serum of the blood and the vitreous body was performed by solid-phase immunosurgical enzyme analysis.Results. Comprehensive analysis of biomicroscopy, ophthalmoscopy, optical coherent tomography, immunological studies revealed no signs of active inflammation; no local and systemic increase in the concentration of acute phase IL-6, a drop in serum IL-2 levels indicated direct effects of immunosuppressive drug combinations used in this study.Conclusion. The use of combined immunosuppressive therapy prevented rejection of xenogenic material in rabbits and obtained evidence of safety of subretinal transplantation of IPSC-RPE suspension both in the healthy eye, and pre-induced atrophy of pigmentary epithelium.

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Injeksi Intravitreal Bevacizumab Dan Laser Fotokoagulasi Pada Branch Retinal Vein Occlucion

Laidasuri

2021

Human Care

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Introduction: Branch retinal vein occlusion (BRVO), occurs at a retinal arteriovenous intersection, is a common cause of retinal vascular disease. The risk factors such as hypertension, arteriosclerosis, diabetes, smoking, hyperlipidemia, and glaucoma. BRVO affects males and females equally and occurs most frequently between the ages of 50 and 70. Vision loss from BRVO is typically due to macular ischemia, macular edema, or complications from neovascular disease Method: Reported a case of BRVO in a man, 50years-old with 10 years hypertension history, came to M.Djamil Hospital) with decreased vision of right eye since one month ago. Visual acuity right eye was 6/18 and visual acuity left eye was 6/6. aa:vv was 1:3,av crossing and venous tortuosity in superotemporal quadran. There was flame shape hemorrages and cotton-wool spot in same area. Foveal reflex was decreased. Central macular thickness was 466 µm on OCT. The management was injection bevacizumab intravitreal and then argon laser photocoagulation 3 weeks later. Result: After 3weeks, macular edema is decreased (234µm) and visual acuity improve up to +3lines of snellen chart (6/6). Conclusion: Combination of bevacizumab intravitreal injection as anti VEGF and laser photocoagulation on leakage area was effective in reducing of macular edema and visual acuity improvement.

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Induction of Branch Retinal Vein Occlusion by Photodynamic Therapy with Rose Bengal in a Rabbit Model

Cited by 3 publications

References 17 publications

Animal Models of Retinal Vein Occlusion

Animal Models of Retinal Vein Occlusion

Clinical and Immunological Analysis of Retinal Pigment Epithelium Transplantation Derived from Induced Pluripotent Stem Cells under Pharmacological Immunosuppression in Rabbits

Injeksi Intravitreal Bevacizumab Dan Laser Fotokoagulasi Pada Branch Retinal Vein Occlucion

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