Induction of budding on chloronemata and caulonemata of the moss, Physcomitrella patens, using isopentenyladenine

Abstract

The bud-inducing effect of the cytokinin N(6)-(Δ(2)-isopentenyl)-adenine (i(6)-Ade) was examined in the moss Physcomitrella patens growing in liquid culture. Under these conditions, buds could be induced on chloronemata as well as on caulonemata. By application of i(6)-Ade, bud-formation was accelerated in both types of tissue. The number of buds, their size and their site of development were dependent on the concentration of the cytokinin in the range of 10(-7) M to 10(-5) M. Moreover, the percentage of caulonema cells increased with a cytokinin concentration of 10(-5) M. These results indicate that chloronema cells may also function as target cells for exogenous cytokinins. The composition of proteins from caulonemata and chloronemata of two different species (P. patens and Funaria hygrometrica), grown on solid medium were compared. No differences could be detected between the protein patterns of caulonemata and chloronemata of the same species while between the two species the differences were obvious.

Glucose inhibits the shoot bud formation in the moss Bryum billarderi

“…Our results showed that BAP and KIN have a concentration-dependent effect on shoot bud induction in B. billarderi after six days of treatment, as was previously reported for the mosses Funaria hygrometrica [8,10,13] and P. patens [9]. BAP caused a higher response than KIN on bud induction, and the former favoured bud formation even at concentrations as small as 0.25 µM (Figure 1).…”

“…The external application of cytokinins to mosses has been shown to favour the transition from filamentous protonemal tissue to gametophores by inducing shoot bud formation [8][9][10]. Further, in the moss Physcomitrella patens, it was reported that the energy growth status promotes protonemal cell differentiation; the high energy condition (addition of 150 mM glucose) favours the caulonema formation, whereas the low energy condition induces chloronema [11].…”

An Eco-physiological and Biotechnological Approach to Conservation of the World-wide Rare and Endangered Aquatic Liverwort Riella helicophylla (Bory et Mont.) Mont.

“…This electrolyte solution was composed of: Ca ( . Three types of solid media, namely MS (Murashige and Skoog 1962), BCD (Sabovljević et al 2009) and KNOP (Reski and Abel, 1985) , and 12000 mg L -1 of agar. Additionally, cultures on MS medium were assayed on half strength MS or supplemented with sucrose 15000 mg L -1 .…”

Cloning and expression analysis of accD gene in Chlorella protothecoides FACHB-2

“…The green microalga C. protothecoides FACHB-2 originally obtained from the Freshwater Algae Culture Collection of the Institute of Hydrobiology, Chinese Academy of Sciences, was cultured with light in KNOP medium (Reski et al, 1985). For autotrophic cultivation, it was inoculated in flask (250 mL) containing 100 mL medium, and cultured in 12 h:12 h light-dark cycle at a light intensity of 40 μmol/m 2 s. For heterotrophic cultivation, it was inoculated in medium containing 10 g/L glucose, and cultivated under darkness.…”