Induction of Cadmium Stress Tolerance in Triticum Aestivum L. By Alfalfa Leaf Extract

Perveen, Shagufta

doi:10.15666/aeer/1405_121136

“…In the current experiment, Cd toxicity markedly reduced the growth attributes of cowpea plants, including shoot length, shoot biomass and leaf area. These results are in conformity with the previous findings on different plant species (Jhanji et al, 2012;Perveen et al, 2016;Sun et al, 2016;Chen et al, 2018). Nonetheless, seed priming with Pro or GB, especially 50mM Pro, improved all measured growth parameters under Cd stress.…”

Section: Discussionsupporting

confidence: 93%

Cadmium Toxicity Alleviates by Seed Priming with Proline or Glycine Betaine in Cowpea ( Vigna unguiculata (L.) Walp.)

Sadeghipour

¹

2020

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C ADMIUM (Cd) as a non-essential metal is a soil pollutant that inhibits plant growth and development. Proline (Pro) and Glycine betaine (GB) are two important compatible solutes that accumulate in response to abiotic stresses in different plant species. Here, a pot experiment was carried out to assess the role of exogenously applied Pro and GB in improving Cd toxicity tolerance in cowpea plants. Seeds were soaked in 0, 25 and 50mM Pro or GB, and then were sown in plastic pots that were irrigated with 0 or 100µM cadmium chloride. Results showed that Cd stress markedly decreased shoot length, shoot biomass, leaf area, chlorophyll value, stomatal conductance and relative water content (RWC) but increased root and shoot Cd levels, malondialdehyde (MDA) content and antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). Nevertheless, seed treatment with Pro or GB reduced Cd toxicity and improved growth attributes via reducing Cd uptake and translocation, increasing the chlorophyll value, stomatal conductance, and RWC as well as more enhancing the activities of antioxidant enzymes and lowering the MDA level. Although both Pro and GB were effective, pre-treatment of seeds with Pro improved Cd stress tolerance better than GB. Thus, seed treatment with Pro (especially 50mM) could be used as an effective method for protecting cowpea plants against Cd toxicity.

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“…[30] found reduced seedling weight when they [31] also reported decreased root and shoot dry weight in peas upon Cd application. In line with our findings, Cd also reduced the growth of other plant species [32][33][34][35]. In this regard, Cd stress inhibits root and stem cell growth by inhibiting cell elongation and cell division via an inhibition activity of the glycolysis pathway.…”

Section: Discussionsupporting

confidence: 91%

Morphological, Biochemical and Quality Control Analysis of Peas Genotypes under Cadmium Toxicity at the Seedling Stage

Manzoor,

Shakeel,

Saeed

et al. 2023

AJAAR

0

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Cadmium toxicity is an emerging problem as it is the most abundant heavy metal in Pakistani soils. Peas are being grown near cities where an elevated level of cadmium persists. So, this study was conducted for the evaluation of 10 peas genotypes (TRIPLET, MID No. 2, Oskar, LM (OSE), METEOR, P-1, Rohina, PEA-09, LM-POIS, and LAMPO) under cadmium stress at the seedling stage in the greenhouse of Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad. There were 3 treatments of cadmium (0, 4, and 8 mg/kg soil) and each treatment was replicated five times in a completely randomized design. Different morphological traits i.e., fresh root weight, fresh seedling weight, root length, seedling length, dry root weight, dry seedling weight and biochemical traits i.e., Cd in the root, shoot, and soil were recorded by atomic absorption spectrometry. Generally, T1 reduced root and shoot parameters while 8 ppm Cd application comparatively enhanced the growth of peas genotypes. LM-POIS and LM (OSE) genotypes performed well under Cd stress and LM-POIS had also an elevated level of Cd in the shoot on the other hand TRIPLET and LAMPO were the least performing genotypes. It is suggested that Cd contamination significantly reduces pea growth; therefore, Cd-tolerant-peas genotypes should be developed or introduced. Further, Cd exposure in the soil should be minimized and already Cd-polluted soils should be remediated.

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“…Ascorbic acid plays as a detoxification agent to destructive damage induced by free radicals (Asada 1999). Perveen et al (2016) documented that alfalfa leaf extract conferred Cd resistance of wheat plant by increasing leaf free proline and total free amino acids thus increased growth and yield of plants under Cd stress.…”

Section: Discussionmentioning

confidence: 99%

Attenuating Effect of an Extract of Cd-Hyperaccumulator Solanum nigrum on the Growth and Physio-chemical Changes of Datura innoxia Under Cd Stress

Abeed

¹

,

Salama

²

2022

J Soil Sci Plant Nutr

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Purpose The use of plant extracts obtained from plants that are highly tolerant to heavy metal toxicity has been beneficial in improving the growth of plants grown under metal toxicity conditions. A lab experiment was performed to elucidate the alleviating role of foliar applied cadmium (Cd)-hyperaccumulator Solanum nigrum (S. nigrum) extract on Datura innoxia (D. innoxia) plants grown under Cd stress (0, 50, and 100 mg Cd kg-1 soil). Methods Growth parameters, photosynthetic pigment content, osmo-metabolic compounds, reduced glutathione and phytochelatins content, oxidative damage, and lignin content and its related enzyme (cell wall-bound peroxidase, POX) were determined. Results Apart from the foliar application response of S. nigrum leaf extract (SNE) in either Cd exposed or non-Cd exposed plants, growth parameters of D. innoxia plant grown under both Cd concentrations (50 and 100 mg Cd kg-1 soil) in terms of root and shoot fresh, dry weight, length, and leaf area were noticeably diminished by 29 and 51%, 29 and 54%, 35 and 70%, 40 and 53%, 30 and 69%, 40 and 60%, and 11 and 23%, respectively, compared with untreated control plants. Foliar delivered SNE secured the photosynthetic pigment, free amino acids, soluble proteins, and soluble sugar content. Additionally, it lessened the adverse effects of Cd stress on D. innoxia plants by curtailing the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA) by 28 and 27%, and 21 and 23%, respectively, compared with the plants subjected to 50 and 100 mg Cd kg-1 only. The findings herein indicated that the plant water extract and their interactions in the investigated Cd rates significantly augmented phenolics, alkaloids, reduced glutathione and phytochelatins content. Cell wall stiffening in D. innoxia indicated that lignin content and POX were significantly higher in plants exposed to 100 mg Cd kg-1 soil displaying increase values of 275 and 300%, respectively, against non-Cd treated control. The magnitude of increment imposed by Cd stress was lessened by using SNE that reflects on adequate cell growth advocated by limited lignification, in terms of lignin content, and downregulated POX activity. Owing to SNE application, root and leaves Cd contents were efficiently reduced reflecting apparent plant liveliness compared with the SNE non-treated Cd-stressed plants. Conclusions The outcomes of this study designate that foliar application of the Cd-hyperaccumulator S. nigrum leaf extract can be counted as an unconventional and innovative approach in the alleviation of Cd stress and can be employed as integrated practice when Cd-contaminated regions were exploited for sustainable agriculture of the multipurpose plants.

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Induction of Cadmium Stress Tolerance in Triticum Aestivum L. By Alfalfa Leaf Extract

Cited by 13 publications

References 27 publications

Cadmium Toxicity Alleviates by Seed Priming with Proline or Glycine Betaine in Cowpea ( Vigna unguiculata (L.) Walp.)

Cadmium Toxicity Alleviates by Seed Priming with Proline or Glycine Betaine in Cowpea ( Vigna unguiculata (L.) Walp.)

Morphological, Biochemical and Quality Control Analysis of Peas Genotypes under Cadmium Toxicity at the Seedling Stage

Attenuating Effect of an Extract of Cd-Hyperaccumulator Solanum nigrum on the Growth and Physio-chemical Changes of Datura innoxia Under Cd Stress

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