Induction of callus and plant regeneration from shoot-tip explants of Dendrobium fimbriatum Lindl. var. oculatum Hk. f.

Roy, Jonojit; Banerjee, Nirmalya

doi:10.1016/s0304-4238(02)00156-5

Cited by 90 publications

(71 citation statements)

References 14 publications

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“…For example, in Dendrobium, NAA plus BAP generated callus, which when subcultured on hormone-free media formed PLBs. 59 Similarly, in Oncidium and Cymbidium, 2,4-D and TDZ treatment generated embryonic callus, which initiated PLBs after subculture on hormone-free media. 60,61 However, root-derived callus from Oncidium generated PLBs that completed somatic embryogenesis on low levels of NAA with higher TDZ concentrations.…”

Section: Protocorm Like-bodies (Plbs)mentioning

confidence: 98%

Role of Auxin in Orchid Development

Novak

Luna

Gamage

2014

Plant Signaling & Behavior

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Section: Protocorm Like-bodies (Plbs)mentioning

confidence: 98%

Role of Auxin in Orchid Development

Novak

Luna

Gamage

2014

Plant Signaling & Behavior

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“…In attempts to accelerate PLBs and plantlet production, synthetic phytohormones, such as thidiazuron (TDZ), indole-3-butyric acid (IBA), 1-naphthalene acetic acid (NAA), and 6-benzyl amino purine (BAP), have been widely applied (Nayak et al, 1997;Roy and Banerjee, 2003;Saiprasad et al, 2004). However, applying synthetic hormones for increasing PLB multiplication leads to induce somaclonal variation (Araditti and Ernest, 1993).…”

Section: Introductionmentioning

confidence: 99%

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Samarfard¹,

Kadir²,

Kadzimin³

et al. 2013

Not Bot Hort Agrobot Cluj

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Chitosan is a carbohydrate polymer derivative of chitin which presents in shell of crustaceans. This biopolymer is a non toxic and environmentally friendly, considered as a plant growth stimulator in some plant species. The present study investigates the effects of chitosan and media types on multiplication and genetic stability of Phalaenopsis gigantea protocorm-like bodies (PLBs). PLBs were inoculated in liquid New Dogashima Medium (NDM) and Vacin and Went (VW) supplemented with various concentrations of chitosan (0, 5, 10, 15, 20 and 25 mg/L). The highest PLB multiplication was observed on VW and NDM supplemented with 10 mg/L chitosan with mean number of PLBs 177 and 147, respectively. Chitosan promoted the formation of juvenile leaves and the highest number was observed in NDM supplemented with 20 mg/L chitosan with mean number of 66 leaves after 8 weeks of culture. Genetic stability was assessed among mother plant and secondary PLBs after 2, 4, 6, and 8 weeks of culture in liquid media. 8 out of 10 ISSR markers produced a total of 275 clear and reproducible bands with mean of 6.9 bands per primer. The secondary PLBs produced during sub-culturing process of chitosan treated liquid culture were genetically uniform and similar to mother plant.

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“…Of the 4 different concentrations (5, 10, 15 and 20 %) used, 15 % gave optimum response with 93 % seed germination (Thomas and Michael 2007). Similarly, CW has been reported to induce seed germination, protocorm and callus regeneration in several orchid species (Kitsaki et al 2004;Roy and Banerjee 2003;Sheelavanthmath et al 2005). The cultured seeds puffed up slowly during the initial stages of development.…”

Section: Resultsmentioning

confidence: 96%

Asymbiotic seed germination and in vitro conservation of Coelogyne nervosa A. Rich. an endemic orchid to Western Ghats

Abraham

Augustine

Thomas

2012

Physiol Mol Biol Plants

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Coelogyne nervosa is an epiphytic orchid endemic to Western Ghats, South India. The mature seeds of C. nervosa were cultured on ½ MS (Murashige and Skoog), MS, Kn (Knudson) and VW (Vacin and Went) media to evaluate the seed germination response. Of the four basal media used, MS medium supported maximum seed germination. Further experiments to enhance seed germination were done on MS medium supplemented with various concentrations (10, 20, 30 and 40 %) of coconut water (CW). Thirty percent CW gave the highest response in terms of percent seed germination (96), fresh weight (7.2 mg/seedling) and protocorm length (15.2 mm). Since CW containing medium did not support further seedling growth, each seedling was isolated and cultured on MS medium supplemented with either BA (6-benzylaminopurine) or Kin (kinetin) alone (1.0-4.0 mg/l each) or in combination with NAA (1-naphthaleneacetic acid; 0.2-1.0 mg/l). Maximum growth was observed on MS medium supplemented with BA (3.0 mg/l) and NAA (0.5 mg/l). On this medium, the seedlings reached an average length of 3.6 cm with 2.8 well expanded green leaves per seedling. Similarly, optimum, healthy, white root induction (3.3 roots/seedlings) was also observed on the same medium. The rooted seedlings were successfully transplanted to pots with 91 % success.The 2-year-old tissue culture derived plants produced normal flowers and fruits.

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Induction of callus and plant regeneration from shoot-tip explants of Dendrobium fimbriatum Lindl. var. oculatum Hk. f.

Cited by 90 publications

References 14 publications

Role of Auxin in Orchid Development

Role of Auxin in Orchid Development

Genetic Stability of In vitro Multiplied Phalaenopsis gigantea Protocorm-like Bodies as Affected by Chitosan

Asymbiotic seed germination and in vitro conservation of Coelogyne nervosa A. Rich. an endemic orchid to Western Ghats

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