Induction of callusogenesis in a culture of isolated seeds of Cistanche Deserticola

Abstract

Cistanche (C. deserticola) - is one of the valuable technical plants of the Kazakhstan flora. The value of cistanche is due to the high content of various polysaccharides, iridoids, and other biologically active substances in the stolons, which are widely used in the eastern countries as feedstock for the production of many pharmacologically active compounds with a wide spectrum of action: increasing tone, potency, antioxidant activity. However, to date, the natural reserves of cistanche are substantially depleted, which makes both the problem of preserving this species and the search for alternative sources of its biologically active substances urgent. It is known that the cell culture of higher plants is an alternative way to obtain rare plant biomass. This paper presents the results of work on optimizing methods for obtaining callus cell cultures from C. deserticola seeds. Several lines of callus cultures of cistanche cells were obtained, growth characteristics were studied. It was established that the proposed method for obtaining callus cultures of cistanche cells is quite effective, the obtained lines have good growth characteristics and differ in color, which is evidence of the synthesis of various secondary metabolites.